Prognostic Value of MicroRNAs in Preoperative Treated Rectal Cancer

Azizian, Azadeh; Epping, Ingo; Jo, Peter; Bernhardt, Markus; Kitz, Julia; Salinas, Gabriela; Wolff, Hendrik A.; Grade, Marian; Beißbarth, Tim; Ghadimi, B. Michael; Gaedcke, Jochen

doi:10.3390/ijms17040568

Cited by 18 publications

(19 citation statements)

References 85 publications

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“…In rectal cancer, miR-92a expression is inversely associated with KLF4 and IQGAP2 expression [14]. miR-573,miR-579 and miR-802 display the significant correlation with overall survival of patients, in addition to, miR-573 is significantly correlated with tumor grade of patients after preoperative chemoradiotherapy [15]. In patients with rectal adenocarcinoma, serum level of miR-125b is significantly over-expressed in pCRT non-responders compared to those responders [16].…”

Section: Introductionmentioning

confidence: 99%

Abnormal expression of mRNA, microRNA alteration and aberrant DNA methylation patterns in rectal adenocarcinoma

Yang

Liu

et al. 2017

PLoS ONE

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AimRectal adenocarcinoma (READ) is a malignancy cancer with the high morbidity and motility worldwide. Our study aimed to explore the potential pathogenesis of READ through integrated analysis of gene expression profiling and DNA methylation data.MethodsThe miRNA, mRNA expression profiling and corresponding DNA methylation data were downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed mRNAs/ miRNAs/methylated regions (DEmRNA/DEmiRNAs) were identified in READ. The negatively correlation of DEmiRNA-DEmRNAs and DNA methylation-DEmRNAs were obtained. DEmRNAs expression was validated through quantitative real-time polymerase chain reaction (qRT-PCR) and microarray expression profiling analyses.Results1192 dysregulated DEmRNAs, 27 dysregulated DEmiRNAs and 6403 aberrant methylation CpG sites were screened in READ compared to normal controls. 1987 negative interaction pairs among 27 DEmiRNAs and 668 DEmRNAs were predicted. 446 genes with aberrant methylation were annotated. Eventually, 50 DEmRNAs (39 down- and 11 up-regulated DEmRNAs) with hypermethylation, synchronously negatively targeted by DEmiRNAs, were identified through the correlation analysis among 446 genes with aberrant methylation and 668 DEmRNAs. 50 DEmRNAs were significantly enriched in cAMP signaling pathway, circadian entrainment and glutamatergic synapse. The validation results of expression levels of DEmRNAs through qRT-PCR and microarray analyses were compatible with our study.Conclusion7 genes of SORCS1, PDZRN4, LONRF2, CNGA3, HAND2, RSPO2 and GNAO1 with hypermethylation and negatively regulation by DEmiRNAs might contribute to the tumorigenesis of READ. Our work might provide valuable foundation for the READ in mechanism elucidation, early diagnosis and therapeutic target identification.

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Section: Introductionmentioning

confidence: 99%

Abnormal expression of mRNA, microRNA alteration and aberrant DNA methylation patterns in rectal adenocarcinoma

Yang

Liu

et al. 2017

PLoS ONE

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“…5,6 In colorectal cancer, a series of miRNAs have been investigated to serve either as a tumor suppressor role or oncogenic driver. 21 Among these miRNAs, miR-143 and miR-145 are initially explored to play a tumor suppressor role in colorectal cancer. 10,22 In addition, miR-101, miR-133b, miR-126 and miR-142-3p are all served as a tumor suppressor in colorectal cancer, while miR-21 and miR-106a expression are significantly higher in the stool of the colorectal cancer patients.…”

Section: Discussionmentioning

confidence: 99%

<p>MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN</p>

Feng¹,

Liu²,

Xu³

et al. 2020

CMAR

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Purpose: Colorectal cancer is one of the most malignant tumors in the world, and the incidence is increasing every year. MicroRNAs (miRNA) are small non-coding RNAs that are involved in a variety of physiological or pathological processes. Abnormal expression of microRNA-802 (miR-802) has been demonstrated in various types of cancer. However, the expression and biological role of miR-802 in human colorectal cancer remain largely unknown. Methods: Here, we used quantitative real-time PCR (qRT-PCR) to measure miR-802 expression levels in colorectal cancer tissues and cell lines. Cell Counting Kit-8 (CCK-8) was used to assess the effect of miR-802 on colorectal cancer cell viability. Migration and invasion assays were performed to determine the effect of miR-802 on metastasis of colon tumor cells by transwell analysis. Luciferase activity assays were used to confirm the target of miR-802. Results: The results show that miR-802 is significantly downregulated in colorectal cancer tissues and cell lines. Overexpression of miR-802 profoundly inhibited viability, migration and invasion of colorectal cancer cells. In addition, we have newly discovered that the Rasassociated nucleus (RAN) is a direct target of miR-802 which could reverse the effects induced by miR-802 overexpression in colorectal cancer cells. Conclusion: In conclusion, our study shows that miR-802 is downregulated in colorectal cancer, and overexpression of miR-802 inhibits colorectal cancer cell viability, migration and invasion by directly targeting RAN.

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“…A study indicated that interrupting miR-579 binding affinity may increase the risk of breast cancer [23]. miR-579 was obviously associated with overall survival and tumor-specific survival of patients with locally advanced rectal cancer [32]. Upregulation of miR-579 could block proliferation, cell cycle, migration and also trigger the apoptosis of glioblastoma multiform cells [24].…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA NEAT1 aggravates osteosarcoma carcinogenesis via regulating the microRNA-579/MMP13 axis

Wang

Zhou

Zhang

et al. 2020

Preprint

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Background: Previous studies have suggested that long non-coding RNAs (lncRNAs) were involved in tumorigenesis in various human carcinomas, including osteosarcoma (OS). However, the expression and specific role of lncRNA NEAT1 in OS remain unknown. The current study aimed at revealing the role of lncRNA NEAT1 and its related mechanism in OS.Methods: Expression profiles of lncRNAs in OS tissues were constructed, and lncRNA NEAT1 expression was verified with RT-qPCR followed by sub-localization. LncRNA-microRNA (miRNA) and miRNA-mRNA interactions were predicted. Validation was performed using dual luciferase reporter gene assay, and gain-and loss-of-function experiments. The effects of lncRNA NEAT1, miR-579 and MMP13 on the proliferation, migration and invasion, epithelial-mesenchymal transition (EMT) of OS cells were detected using colony formation, cell counting kit-8 (CCK-8), Transwell assays and Western blot analysis. Results: LncRNA NEAT1 overexpression was observed in OS tissues and cell lines which located in the cytoplasm. Transfection-induced downregulation of lncRNA NEAT1/MMP13 or overexpression of miR-579 blocked the progression of OS cells. LncRNA NEAT1 promotes MMP13 through sponging miR-579.Conclusion: LncRNA NEAT1 might be beneficial for OS aggravation via sponging miR-579 and facilitating MMP13 expression, which represents a candidate marker and target for OS therapy.clarify the invisible mechanism underlying tumorigenesis and to develop novel molecular targets of OS.Long non-coding RNAs (lncRNAs), surpassing over 200 nucleotides in length, are a prominent class of RNAs exerting a crucial regulatory potential in tumor cell behaviors [6]. Increasing evidence has illustrated that aberrantly expressed lncRNAs were highly associated with the occurrence and development of many human malignancies, including OS [7-9]. Nuclear enriched abundant transcript 1 (NEAT1) is a lncRNA transcribed from the diverse endocrinal neoplasia locus [10]. The interaction of lncRNA NEAT1 has been documented in glioma, prostate cancer and lung adenocarcinoma [11][12][13].LncRNA NEAT1 was also suggested as a tumor promotor in OS by several studies [14][15][16].MicroRNAs (miRNAs) are tiny (~ 22 nucleotides) endogenous non-coding RNA molecules which act at the post-transcriptional level and regulate mRNA expression [17]. They are involved in multiple complicated cellular behaviors [18]. Importantly, emerging evidence has pointed out that miRNAs participate in OS, such as miR-18a-5p, miR-671-5p, miR-1301, and miR-212 [19-22]. MiR-579 was reported to be associated with malignancies, such as breast cancer, ovarian cancer, and human glioblastoma [23][24][25]. However, its function in OS deserves further studies.LncRNAs are proved to serve as competing endogenous RNAs (ceRNAs), which sponges certain miRNAs to mediate its target gene, thus changing the post-transcriptional regulation [1]. However, the concrete influence that lncRNA NEAT1 and miR-579 exerts on OS, especially their interaction is still to be elucidated. In this experimen...

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Prognostic Value of MicroRNAs in Preoperative Treated Rectal Cancer

Cited by 18 publications

References 85 publications

Abnormal expression of mRNA, microRNA alteration and aberrant DNA methylation patterns in rectal adenocarcinoma

Abnormal expression of mRNA, microRNA alteration and aberrant DNA methylation patterns in rectal adenocarcinoma

<p>MiR-802 Suppresses Colorectal Cancer Cell Viability, Migration and Invasion by Targeting RAN</p>

Long noncoding RNA NEAT1 aggravates osteosarcoma carcinogenesis via regulating the microRNA-579/MMP13 axis

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