Prognostic value of S-100 immunostaining in tumour cells of non-small cell lung cancer

Jassem, Ewa; Serkies, Krystyna; Dziadziuszko, Rafał; Drozdowska, Adrianna; Kobierska‐Gulida, Grażyna; Skokowski, Jan; Góźdż, Stanisław; Urbaniak, Anna; Sygut, Jacek; Jassem, J

doi:10.1080/13547500600652277

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…A 12 years old boy died shortly after diagnosis from an aggressive s100p+ CD56+ non-nasal T cell lymphoproliferative disorder presenting with splenic and marrow involvement [33]. In non haematological malignancies, s100p expression was significantly associated with decreased survival and was an independent predictor of poor prognosis in non-small cell lung cancer [34]. …”

Section: Discussionmentioning

confidence: 99%

Smooth muscle actin and s100p on non germinal centre diffuse large B cell lymphoma are adverse prognostic factors: pilot study

All

2007

Diagn Pathol

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Section: Discussionmentioning

confidence: 99%

Smooth muscle actin and s100p on non germinal centre diffuse large B cell lymphoma are adverse prognostic factors: pilot study

All

2007

Diagn Pathol

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Differential expression of calcium-related genes in gastric cancer cells transfected with cellular prion protein

Liang

Luo

Ning

et al. 2007

Biochem. Cell Biol.

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The prion protein (PrPC) has a primary role in the pathogenesis of transmissible spongiform encephalopathies, which causes prion disorders partially due to Ca2+ dysregulation. In our previous work, we found that overexpressed PrPC in gastric cancer was involved in apoptosis, cell proliferation, and metastasis of gastric cancer. To better understand how PrPC acts in gastric cancer, a human microarray was performed to select differentially regulated genes that correlate with the biological function of PrPC. The microarray data were analyzed and revealed 3798 genes whose expression increased at least 2-fold in gastric cancer cells transfected with PrPC. These genes encode proteins involved in several aspects of cell biology, among which, we specially detected molecules related to calcium, especially the S100 calcium-binding proteins, and found that PrPC upregulates S100A1, S100A6, S100B, and S100P but downregulates CacyBP in gastric cancer cells. We also found that intracellular Ca2+ levels in cells transfected with PrPC increased, whereas these levels decreased in knockdowns of these cells. Taken together, PrPC might increase intracellular Ca2+, partially through calcium-binding proteins, or PrPC might upregulate the expression of S100 proteins, partially through stimulating the intracellular calcium level in gastric cancer. Though the underlying mechanisms need further exploration, this study provides a new insight into the role of PrPC in gastric cancer and enriches our knowledge of prion protein.

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Large-scale proteomic identification of S100 proteins in breast cancer tissues

et al. 2010

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BackgroundAttempts to reduce morbidity and mortality in breast cancer is based on efforts to identify novel biomarkers to support prognosis and therapeutic choices. The present study has focussed on S100 proteins as a potentially promising group of markers in cancer development and progression. One reason of interest in this family of proteins is because the majority of the S100 genes are clustered on a region of human chromosome 1q21 that is prone to genomic rearrangements. Moreover, there is increasing evidence that S100 proteins are often up-regulated in many cancers, including breast, and this is frequently associated with tumour progression.MethodsSamples of breast cancer tissues were obtained during surgical intervention, according to the bioethical recommendations, and cryo-preserved until used. Tissue extracts were submitted to proteomic preparations for 2D-IPG. Protein identification was performed by N-terminal sequencing and/or peptide mass finger printing.ResultsThe majority of the detected S100 proteins were absent, or present at very low levels, in the non-tumoral tissues adjacent to the primary tumor. This finding strengthens the role of S100 proteins as putative biomarkers. The proteomic screening of 100 cryo-preserved breast cancer tissues showed that some proteins were ubiquitously expressed in almost all patients while others appeared more sporadic. Most, if not all, of the detected S100 members appeared reciprocally correlated. Finally, from the perspective of biomarkers establishment, a promising finding was the observation that patients which developed distant metastases after a three year follow-up showed a general tendency of higher S100 protein expression, compared to the disease-free group.ConclusionsThis article reports for the first time the comparative proteomic screening of several S100 protein members among a large group of breast cancer patients. The results obtained strongly support the hypothesis that a significant deregulation of multiple S100 protein members is associated with breast cancer progression, and suggest that these proteins might act as potential prognostic factors for patient stratification. We propose that this may offer a significant contribution to the knowledge and clinical applications of the S100 protein family to breast cancer.

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Prognostic value of S-100 immunostaining in tumour cells of non-small cell lung cancer

Cited by 4 publications

References 27 publications

Smooth muscle actin and s100p on non germinal centre diffuse large B cell lymphoma are adverse prognostic factors: pilot study

Smooth muscle actin and s100p on non germinal centre diffuse large B cell lymphoma are adverse prognostic factors: pilot study

Differential expression of calcium-related genes in gastric cancer cells transfected with cellular prion protein

Large-scale proteomic identification of S100 proteins in breast cancer tissues

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