Program for Intensive Production of Virus-Free Garlic Plants

Conci, V. C.; Perotto, María Cecilia; Cafrune, E. E.; Lunello, Pablo

doi:10.17660/actahortic.2005.688.25

Cited by 22 publications

(14 citation statements)

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“…Huruf yang sama di atas balok data menunjukkan tidak berbeda nyata pada tiap kelompok termoterapi menurut uji DMRT pada α 5%. Conci et al (2005) yang melakukan penelitian pada tanaman bawang putih, eksplan meristem yang diberi perlakuan suhu 36 °C selama 30-40 hari dapat tumbuh sekitar 55-57% dibandingkan dengan eksplan yang tidak diberi perlakuan dapat tumbuh sekitar 80-90%. Mahmoud et al (2009) melaporkan bahwa tingkat regenerasi semakin menurun pada tanaman kentang yang diberi perlakuan suhu 37 °C selama 40 hari secara in vitro.…”

Section: Termoterapi Dan Elektroterapi Padaunclassified

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Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus

Nasution

Dinarti

Hidayat

2018

J Fitopatol Indones

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Infection of Onion yellow dwarf virus (OYDV) are reported causing problems in garlic production. Planting virus-free bulbs might help reduce viral disease incidence in the field. This research was aimed to develop method for eliminating OYDV from garlic bulbs using combination of electrotherapy (0, 5, 10, 15, and 20 mA each for 10 minutes) and thermotherapy (23, 28, 33, 38°C each for 4 weeks). Two garlic cultivars, i.e. Sangga Sembalun and Lumbu Hijau were used as seed bulbs for OYDV elimination tests. Virus infection was confirmed using transcription-polymerase chain reaction (RT-PCR). The result showed that thermotherapy at 33 °Cwas the best method to eliminate OYDV in garlic although the efficiency was not the same for all cultivars. The efficiency reached up to 60% for cv. Lumbu Hijau, whereas for cv. Sangga Sembalun only reached up to 40%. Electrotherapy alone or in combination with thermotherapy were not able to produce OYDV-free plantlets.

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Section: Termoterapi Dan Elektroterapi Padaunclassified

“…Menurut Conci et al (2005), efisiensi untuk mendapatkan tanaman bebas virus bergantung dari jenis virus target dan kultivar bawang putih yang digunakan. Ghaemizadeh et al (2014) berhasil memperoleh tanaman bebas OYDV dengan efisiensi 77% melalui perlakuan suhu 36 o C dikombinasikan dengan kultur stem-disc.…”

Section: Termoterapi Dan Elektroterapi Padaunclassified

Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus

Nasution

Dinarti

Hidayat

2018

J Fitopatol Indones

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“…This, coupled with the use of virus-free garlic plants, directly influences incidence values. Argentina has a very important program for virus-free garlic plant production and for distribution of healthy plants, in the most important garlic-growing areas (Conci, 1997;Conci et al, 2005).…”

Section: Departmentmentioning

confidence: 99%

“…Therefore, a virus-free garlic plant production system has been implemented, which produces mother plants in specialized laboratories (Conci & Nome, 1991;Conci et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates

Torrico

Celli

Conci

et al. 2015

Pesq. agropec. bras.

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-The objective of this work was to estimate the incidence and prevalence of Garlic common latent virus (GarCLV) in the main production regions of garlic (Allium sativum) in Argentina, and to perform phylogenetic and recombination analyses in isolates from these regions. Leaf samples (3,050) were taken from four garlic commercial types, in 13 departments of the four main garlic-producing provinces of Argentina, in a 1,175-ha sampling area. Virus infection was evaluated with DAS-Elisa test using specific antiserum, and the phylogenetic and recombination analyses were done with capsid protein (CP) nucleotide sequence of seven GarCLV isolates from the provinces. The incidence of GarCLV in the evaluated provinces varied between 6.7 and 22% of the samples, whereas the prevalence varied between 52.6 and 70%. In the analysis of garlic commercial types, Morado showed the highest incidence of the virus, in the province of San Juan, whereas Rosado Paraguayo had the lowest incidence, in the province of Cordoba. Nucleotide identity in the CP sequences ranged between 80.3 and 97.6%. The phylogenetic analysis shows the presence of two main groups of GarCLV and of a possible third group that would include only a German isolate. The recombination analysis between isolates from different parts of the world evidences the presence of recombinant isolates from Poland and Australia.

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“…Gejala serangan virus pada tanaman bawang merah bervariasi bergantung pada jenis virus, umur tanaman, inang, vektor, dan faktor lingkungan. Menurut Melo Filho et al (2006) dan Conci et al (2004), penggunaan umbi benih bawang putih sehat akan meningkatkan hasil umbi lebih dari 100% dibandingkan umbi benih yang terinfeksi virus dapat menurunkan hasil berat umbi 66-216% dan diameter umbi 13-51%.…”

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Deteksi Penyakit Virus Pada Bawang Merah Asal Kabupaten Brebes dan Cirebon dan Daerah Pencarnya Menggunakan Teknik RT-PCR (Detection of Viral Diseases on Shallot from Brebes and Cirebon Districts and their Spread Using the RT-PCR Techniques)

Gunaeni¹,

Karyadi²,

Adiyoga³

2019

J.Hort

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Bawang merah (Allium cepa var. ascalonicum) merupakan salah satu komoditas penting sayuran. Salah satu masalah yang dihadapi dalam budidaya bawang merah adalah adanya penyakit yang disebabkan oleh virus yang dapat menurunkan kualitas dan kuantitas hasil. Penelitian bertujuan mengetahui kelompok virus yang menginfeksi bawang merah dan daerah pencarnya di Kabupaten Brebes dan Cirebon. Kegiatan dilakukan dengan pengambilan sampel tanaman pada bulan September 2013 (musim kemarau) dan April 2014 (musim hujan). Identifikasi virus dilakukan di Laboratorium Virologi Balai Penelitian Tanaman Sayuran menggunakan teknik RT-PCR. Hasil penelitian menunjukkan bahwa: (1) tingginya insiden gejala virus bergantung pada pola tanam, penggunaan varietas, umur tanaman, dan kondisi lingkungan di sekitar tanaman, (2) umumnya petani di Kabupaten Brebes dan Cirebon menanam bawang merah varietas Bima Curut, (3) daerah pencar kelompok Potyvirus, Allexivirus, dan Carlavirus cukup luas di Kabupaten Brebes dan Cirebon, (4) terdeteksi dari kelompok sampel Kabupaten Brebes Potyvirus 92,30%, Allexivirus 92,50%, dan Carlavirus 99%, dan (5) terdeteksi dari kelompok sampel asal Kabupaten Cirebon Potyvirus 96,43%, Allexivirus 96,15%, dan Carlavirus 93%. Implikasi dari infeksi ketiga kelompok virus tersebut pada tanaman bawang merah dapat menurunkan produksi 21,57–54,90%.KeywordsAllium cepa var. ascalonicum; Deteksi; Potyvirus; Allexivirus; CarlavirusAbstractShallot (Allium cepa var. ascalonicum) is one of the important vegetable commodity. The problems encountered in the cultivation of shallot is the disease caused by a virus which can reduce the quality and yield quantity. This study aimed to determine the group of viruses that infect shallot and geographycal distribution in Brebes and Cirebon Districts. The activities carried out by plant sampling in September 2013 (dry season) and April 2014 (rainy season). Identification of virus carried in the Virology Laboratory of Indonesian Vegetables Research Institute to perform testing using RT-PCR. The results showed that: (1) the high incidence of viral symptoms depend on cropping patterns, use of improved varieties, plant age, environmental conditions around the plant, (2) generally famers in Brebes and Cirebon Districts planted Bima Curut varieties, (3) geographycal distribution Potyvirus group, Allexivirus, and Carlavirus quite extensive in Brebes and Cirebon regions, (4) detected viruses from samples of Brebes District : Potyvirus group 92.30%, Allexivirus 92.50%, and Carlavirus 99%, and (5) detected viruses from samples of Cirebon District : Potyvirus group 96.43%, Allexivirus 96.15%, and Carlavirus 93%. The implications of the infection of the above three groups of viruses on the plant can decrease the production of shallots 21.57–51.90%.

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Program for Intensive Production of Virus-Free Garlic Plants

Cited by 22 publications

References 0 publications

Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus

Pengaruh Elektroterapi dan Termoterapi secara in Vitro terhadap Eliminasi Onion yellow dwarf virus

Incidence of Garlic common latent virus in Argentina, and phylogenetic and recombination analyses of isolates

Deteksi Penyakit Virus Pada Bawang Merah Asal Kabupaten Brebes dan Cirebon dan Daerah Pencarnya Menggunakan Teknik RT-PCR (Detection of Viral Diseases on Shallot from Brebes and Cirebon Districts and their Spread Using the RT-PCR Techniques)

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