Programa informático aplicado al estudio temporal de procesos patológicos en poblaciones animales

Ortega, C.; Blas, Ignacio de; Mc, Simón; Gironés, Olivia; García, Juan Francisco Blanco; Jl, Alonso Calderón; Jl, Muzquiz; Ruiz, Ignacio Martínez

doi:10.20506/rst.19.3.1245

Cited by 3 publications

(1 citation statement)

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“…Sample size was determined considering Na concentration, since it is the most important public health parameter in this study. A minimum of 5 legs per group are necessary to detect a difference of 10 and 8% Na in ham, with 80% power and 95% confidence, for a one-tailed statistical test [14,27,31]. The pigs were raised in the Linares province (latitude-36.1431084 and longitude-71.8260498), Chile.…”

Section: Sample Size and Unit Analysismentioning

confidence: 99%

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2020

OAJBS

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Section: Sample Size and Unit Analysismentioning

confidence: 99%

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2020

OAJBS

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Frequency of DEA 1 antigen in 1037 mongrel and PUREBREED dogs in ITALY

Carli

Carminato²,

Ravagnan

et al. 2017

BMC Vet Res

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BackgroundThe prevalence of dog erythrocyte antigen (DEA 1) in canine population is approximately 40–60%. Often data are limited to a small number of breeds and/or dogs. The aims of this study were to evaluate frequency of DEA 1 in a large population of purebred and mongrel dogs including Italian native breeds and to recognize a possible association between DEA 1 and breed, sex, and genetic and phenotypical/functional classifications of breeds. Frequencies of DEA 1 blood group collected from screened/enrolled blood donors and from healthy and sick dogs were retrospectively evaluated. The breed and the sex were recorded when available. DEA 1 blood typing was assessed by immunocromatographic test on K3EDTA blood samples. The prevalence of DEA 1 antigen was statistically related to breed, gender, Fédération Cynologique Internationale (FCI) and genotypic grouping.ResultsSixty-two per cent dogs resulted DEA 1+ and 38% DEA 1-. DEA 1- was statistically associated with Dogo Argentino, Dobermann, German Shepherd, Boxer, Corso dogs, the molossian dogs, the FCI group 1, 2 and 3 and the genetic groups “working dogs” and “mastiff”. DEA 1+ was statistically associated with Rottweiler, Briquet Griffon Vendéen, Bernese mountain dog, Golden Retriever, the hunting breeds, the FCI group 4, 6, 7 and 8 and the genetic groups “scent hounds” and “retrievers”. No gender association was observed.ConclusionsData obtained by this work may be clinically useful to drive blood donor enrollment and selection among different breeds.

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Seroprevalence and Genotypic Analysis of Ehrlichia canis Infection in Dogs and Humans in Cauca, Colombia

Forero-Becerra

Patel

Martínez-Diaz

et al. 2021

The American Journal of Tropical Medicine and Hygiene

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Ehrlichia canis infections have been reported in humans in Venezuela and Costa Rica. In this study, 506 healthy residents and 114 dogs from four municipalities (Cauca, Colombia) were surveyed and blood samples collected. Antibodies to E. canis in human and canine sera were evaluated using the Tandem repeat protein 19 (TRP19) peptide ELISA and indirect immunofluorescence assay (IFA). Ehrlichia canis TRP19 antibodies were detected in only 1/506 human sera, but the single positive sample was negative by IFA. The majority (75/114; 66%) of dogs surveyed had antibodies to the E. canis TRP19 peptide by ELISA, and eight randomly selected sera were further confirmed by E. canis IFA. Genomic DNA samples obtained from 73 E. canis TRP19 ELISA–positive dog blood samples were examined by PCR targeting the 16S rRNA gene. Ehrlichia canis 16S rRNA was amplified in 30 (41%) of the dogs, and 16 amplicons were selected for DNA sequencing, which confirmed that all were E. canis. A second PCR was performed on the 16 confirmed E. canis 16S rRNA PCR–positive samples to determine the TRP36 genotype by amplifying the trp36 gene. TRP36 PCR amplicon sequencing identified nine dogs infected with the U.S. E. canis TRP36 genotype (56%), one dog with the Brazilian genotype (6%), and six dogs with the Costa Rican genotype (38%). Moreover, these molecular genotype signatures were consistent with serologic analysis using TRP36 genotype–specific peptides. Notably, there was no serologic evidence of E. canis infection in humans, suggesting that E. canis infection in dogs in Cauca is not associated with zoonotic human infection.

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Programa informático aplicado al estudio temporal de procesos patológicos en poblaciones animales

Cited by 3 publications

References 0 publications

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Frequency of DEA 1 antigen in 1037 mongrel and PUREBREED dogs in ITALY

Seroprevalence and Genotypic Analysis of Ehrlichia canis Infection in Dogs and Humans in Cauca, Colombia

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