Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

Dadwal, Anica; Sharma, Shilpa; Satyanarayana, T.

doi:10.3389/fmicb.2020.01387

Cited by 55 publications

(21 citation statements)

References 201 publications

(235 reference statements)

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“…Microorganisms often lack the high yields and productivity required for industrial applications [ 229 ]. Large amounts of stable and active enzymes can be obtained by genetic engineering, such as using a strong promoter or overexpression in host cells, which can not only reduce the cost of enzymes during the pretreatment and saccharification process but also pave the way for a one-step conversion strategy from lignocellulosic materials to high-value products [ 233 – 235 ].…”

Section: Challenges In the Valorization Of Plant Biomassmentioning

confidence: 99%

Recent advances in the valorization of plant biomass

Ning

Yang

et al. 2021

Biotechnol Biofuels

178

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Plant biomass is a highly abundant renewable resource that can be converted into several types of high-value-added products, including chemicals, biofuels and advanced materials. In the last few decades, an increasing number of biomass species and processing techniques have been developed to enhance the application of plant biomass followed by the industrial application of some of the products, during which varied technologies have been successfully developed. In this review, we summarize the different sources of plant biomass, the evolving technologies for treating it, and the various products derived from plant biomass. Moreover, the challenges inherent in the valorization of plant biomass used in high-value-added products are also discussed. Overall, with the increased use of plant biomass, the development of treatment technologies, and the solution of the challenges raised during plant biomass valorization, the value-added products derived from plant biomass will become greater in number and more valuable.

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Section: Challenges In the Valorization Of Plant Biomassmentioning

confidence: 99%

Recent advances in the valorization of plant biomass

Ning

Yang

et al. 2021

Biotechnol Biofuels

178

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“…The thermostable cellulases are in great demand in various biotechnological industries including bioethanol production (Suresh et al 2021 ; Turner et al 2007 ). Over the years, several investigations have been carried out for evolving efficient thermostable cellulases through various genetic/protein engineering techniques (Dadwal et al 2020 ). To fulfil the immense demand for the thermostable cellulases for industrial applications, heterologous expression has become the crucial technique that often significantly improves protein expression levels (Lambertz et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharification

2021

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A codon optimized cellobiohydrolase (CBH) encoding synthetic gene of 1188 bp from a thermophilic mold Myceliophthora thermophila (MtCel6A) was cloned and heterologously expressed in Escherichia coli for the first time. In silico analysis suggested that MtCel6A is a GH6 CBH and belongs to CBHII family, which is structurally similar to Cel6A of Humicola insolens. The recombinant MtCel6A is expressed as active inclusion bodies, and the molecular mass of the purified enzyme is ~ 45 kDa. The rMtCel6A is active in a wide range of pH (4–12) and temperatures (40–100 °C) with optima at pH 10.0 and 60 °C. It exhibits T1/2 of 6.0 and 1.0 h at 60 and 90 °C, respectively. The rMtCel6A is an extremozyme with organic solvent, salt and alkali tolerance. The Km, Vmax, kcat and kcat/Km values of the enzyme are 3.2 mg mL−1, 222.2 μmol mg−1 min−1, 2492 s−1 and 778.7 s−1 mg−1 mL−1, respectively. The product analysis of rMtCel6A confirmed that it is an exoenzyme that acts from the non-reducing end of cellulose. The addition of rMtCel6A to the commercial cellulase mix (Cellic CTec2) led to 1.9-fold increase in saccharification of the pre-treated sugarcane bagasse. The rMtCel6A is a potential CBH that finds utility in industrial processes such as in bioethanol, paper pulp and textile industries. Graphical Abstract

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“…This strategy is based on the mutation of specific residues or a stretch of residues at a specific position in the structure of a protein (Fig. 3) and involves three steps: selection of appropriate enzyme, identification of the residues to be mutated, and characterization of the mutants (26)(27)(28). The rational design approach usually yields smaller libraries than those generated in directed evolution; however, prior knowledge about protein structure, function, and catalytic site is required for the selection of the residues to be mutated (28,29).…”

Section: Figmentioning

confidence: 99%

Enzyme engineering and its industrial applications

Amatto

Rosa-Garzon

Simões

et al. 2021

Biotech and App Biochem

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Recently, there has been an increase in the demand for enzymes with modified activity, specificity, and stability. Enzyme engineering is an important tool to meet the demand for enzymes adjusted to different industrial processes. Knowledge of the structure and function of enzymes guides the choice of the best strategy for engineering enzymes. Each enzyme engineering strategy, such as rational design, directed evolution, and semi-rational design, has specific applications, as well as limitations, which must be considered when choosing a suitable strategy. Engineered enzymes can be optimized for different industrial applications by choosing the appropriate strategy. This review features engineered enzymes that have been applied in food, animal feed, pharmaceuticals, medical applications, bioremediation, biofuels, and detergents.

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Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification

Cited by 55 publications

References 201 publications

Recent advances in the valorization of plant biomass

Recent advances in the valorization of plant biomass

Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharification

Enzyme engineering and its industrial applications

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