Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis

Chen, Huayou; Ullah, Jawad; Jia, Jinru

doi:10.1159/000475177

Cited by 269 publications

(34 citation statements)

References 58 publications

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“…The enormous stress resistance of endospores can be coopted to improve the stability and promote the reusability of enzymes in complex environments [ 94 ]. In addition, spore surface display technology has recently been successfully applied in the production of protein polymers, vaccines and industrial enzymes [ 95 ]. Enzymes such as lipase and chitinase have been successfully expressed on the surface of endospores [ 94 ].…”

Section: Application Of Bacillus Subtilis In Amentioning

confidence: 99%

Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine

Chuan²,

Fang³

et al. 2020

Microb Cell Fact

280

142

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Due to its clear inherited backgrounds as well as simple and diverse genetic manipulation systems, Bacillus subtilis is the key Gram-positive model bacterium for studies on physiology and metabolism. Furthermore, due to its highly efficient protein secretion system and adaptable metabolism, it has been widely used as a cell factory for microbial production of chemicals, enzymes, and antimicrobial materials for industry, agriculture, and medicine. In this mini-review, we first summarize the basic genetic manipulation tools and expression systems for this bacterium, including traditional methods and novel engineering systems. Secondly, we briefly introduce its applications in the production of chemicals and enzymes, and summarize its advantages, mainly focusing on some noteworthy products and recent progress in the engineering of B. subtilis . Finally, this review also covers applications such as microbial additives and antimicrobials, as well as biofilm systems and spore formation. We hope to provide an overview for novice researchers in this area, offering them a better understanding of B. subtilis and its applications.

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Section: Application Of Bacillus Subtilis In Amentioning

confidence: 99%

Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine

Chuan²,

Fang³

et al. 2020

Microb Cell Fact

280

142

View full text Add to dashboard Cite

show abstract

“…it is the mainstream of B. subtilis spore surface display technology (Chen et al 2017b;Kim and Schumann 2009). In this review, we summarize the application of genetic recombination-based spore surface display technology in many fields, discuss new and developing research, and determine the future prospects of the technology.…”

Section: Introductionmentioning

confidence: 99%

Progress in research and application development of surface display technology using Bacillus subtilis spores

Lin

Yuan

et al. 2020

Appl Microbiol Biotechnol

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Bacillus subtilis is a widely distributed aerobic Gram-positive species of bacteria. As a tool in the lab, it has the advantages of nonpathogenicity and limited likelihood of becoming drug resistant. It is a probiotic strain that can be directly used in humans and animals. It can be induced to produce spores under nutrient deficiency or other adverse conditions. B. subtilis spores have unique physical, chemical, and biochemical characteristics. Expression of heterologous antigens or proteins on the surface of B. subtilis spores has been successfully performed for over a decade. As an update and supplement to previously published research, this paper reviews the latest research on spore surface display technology using B. subtilis. We have mainly focused on the regulation of spore coat protein expression, display and application of exogenous proteins, and identification of developing research areas of spore surface display technology.

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“…2015; Chen et al . 2017), there is no systematic summary and description of their application in aquaculture. Therefore, this paper focuses on the system of probiotics commonly used in aquaculture for surface display and its application in aquaculture.…”

Section: Introductionmentioning

confidence: 99%

Surface display system for probiotics and its application in aquaculture

Yao

Yang

Gao

et al. 2020

Reviews in Aquaculture

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Aquaculture is a fast‐growing and rapidly expanding industry in the world. Probiotics are widely used in aquaculture and have provided benefits to aquatic animal health, and it is also a promising alternative to antibiotics for control of fish diseases. With the development of biotechnology, new expression systems and novel techniques for the surface display of heterologous proteins in surface of probiotics cells have been developed. This review provides an overview of the systems and strategies for displaying functional proteins on the surface of probiotics commonly used in aquaculture, which are Bacillus subtilis, Bacillus thuringiensis, lactic acid bacteria represented by Lactococcus lactis and yeast. Their applications in aquaculture especially for oral vaccine development afforded by this technology and prospects and challenges associated with this technology are also highlighted.

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Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis

Cited by 269 publications

References 58 publications

Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine

Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine

Progress in research and application development of surface display technology using Bacillus subtilis spores

Surface display system for probiotics and its application in aquaculture

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