Progress in cellular engineering of plants: biochemical and genetic assessment of selectable markers from cultured cells

Negrutiu, Ioan; Jacobs, M.; Cattoir-Reynaerts, A.

doi:10.1007/bf00017783

Cited by 24 publications

(4 citation statements)

References 32 publications

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“…The appearance of variant cell lines with a persistent resistance to 5-MT levels lower than those originally included in the selection medium could be explained by the assumption that the activity of this analogue in the medium decreases in time (Cella and ladarola ]983). Phenylalanine accumulating 5-MT resistant cell lines have previously been described for Arabidopsis thaliana (Negrutiu 1978) and for rice (Wakasa 1983), but 5-MT resistant cell lines accumulating high levels of tyrosine or tyrosine and phenylalanine simultaneously as described here, have to the authors' knowledge not been reported before. In extracts of 5-MT resistant cell lines of carrot ( Cella and ladarola 1983) besides tryptophan accumulation higher amounts of proline have been measured and cross resistance with azetine-2-carboxylic acid (a2C) , an analogue of proline, was found.…”

Section: Concmentioning

confidence: 81%

Phenylalanine and tyrosine accumulating cell lines of a dihaploid potato selected by resistance to 5-methyltryptophan

1985

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The selection and characterization of 5-methyltryptophan (5-MT) resistant cell lines is described in a dihaploid potato, clone H(2)578(007). The frequency of resistant calli was increased by treatment of a cell suspension with N-ethyl-N-nitroso-urea. Two lines, 5-MT-21 and 5-MT-26, accumulated tyrosine (160 nMol and 1546 nMol/g fresh callus), and the third, 5-MT-27, accumulated both tyrosine (841 nMol/g fresh callus) and phenylalanine (451 nMol/g fresh callus). In the wildtype tyrosine and phenylalanine content was 65 nMol and 42 nMol/g callus, respectively. The tryptophan content of line 5-MT-26 was significantly increased, from 20 nMol/g to 76 nMol/g fresh callus. The total free amino acid content of the three variant cell lines was higher than that of the wildtype. Variant cell lines 5-MT-21, -26 and -27 showed a low degree of resistance to 5-MT, when grown on a selective medium and were cross-resistant to parafluorophenylalanine and 3-fluorotyrosine, analogues of phenylalanine and tyrosine.

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Section: Concmentioning

confidence: 81%

Phenylalanine and tyrosine accumulating cell lines of a dihaploid potato selected by resistance to 5-methyltryptophan

1985

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“…Selectable markers have been evaluated for several species (Hauptmann et al 1988, Negrutiu et al 1984, but an ideal marker suitable for all cases does not yet exist. We have studied the interaction between sunflower genotypes and three markers with two different mechanisms of action (inhibition of translation and inhibition of glutamine synthetase).…”

Section: Discussionmentioning

confidence: 99%

Genotype and composition of culture medium are factors important in the selection for transformed sunflower (Helianthus annum)callus

Escandón

Hahne

1991

Physiologia Plantarum

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Transformed tissues can be efficiently selected with the help of selectable markers. We studied the influence of genotype, culture medium, and chemical nature of selectable markers on the callus formation of sunflower (Helianthus annuus L.) hypocotyl explants during the selection process. Kanamycin was found not to be a suitable marker for sunflower transformation, while paromomycin and phosphinotri‐cin are both useful. Because the mechanism of action of these inhibitors is intimately connected with the physiological reactions of the cultured tissues, we investigated the influence of key media components on the efficiency of selection of transformed callus on phosphinotricin. Auxin, cytokinin, and gibberellic acid, as well as nitrate concentration, all had a strong influence on the spontaneous resistance of untrans‐formed sunflower hypocotyl explants against this herbicide. The number of transformed cells varied in function of the growth regulator balance. Possible reasons for this media dependence are discussed.

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“…Cell culture techniques have been applied to select mutants in higher plants (MALI-GA, 1984;NEGRUTIU et al, 1984). At our laboratory a good fertile diploid potato clone was selected for its capacity to proliferate vigorously in cell culture and to regenerate even after more than 1 year in culture (JACOBSEN et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Increased frost tolerance and amino acid content in leaves, tubers and leaf callus of regenerated hydroxyproline resistant potato clones

Swaaij¹,

Nijdam²,

Jacobsen³

et al. 1987

Euphytica

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Sohumtuberosum, potato, amino acids, amino acid analogue resistance, frost tolerance, plant regeneration, proline, somaclonal variation. SUMMARY A number of previously selected hydroxyproline (hyp) resistant cell lines of a diploid potato (Solunum tuberosum L., clone H2578, 2n=2x=24) could be regenerated into plants which were further analysed. Hyp resistance, although lower than in the originally selected calli, was still present in regenerated shoots and in callus initiated from these shoots and it was not lost upon (mini)tuber propagation. Regenerated shoots showed a wide range of phenotypic variation. The chromosome number, analysed in 4 clones, appeared to be hypotetraploid (44 or 45). Tuber-propagated regenerants generally showed increased frost tolerance both at the plant and the cell level. In leaves this seemed to be associated with increased levels of both proline and total amino acid content. However, in callus only the total amino acid content but not proline was still elevated. In the wild type the frost tolerance of the tubers appeared to be lower than that of the leaves. Between tubers of wild type and the hyp resistant regenerants no differences in frost tolerance were found, although proline and total amino acid content tended to be higher in tubers from the regenerants.

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Progress in cellular engineering of plants: biochemical and genetic assessment of selectable markers from cultured cells

Cited by 24 publications

References 32 publications

Phenylalanine and tyrosine accumulating cell lines of a dihaploid potato selected by resistance to 5-methyltryptophan

Phenylalanine and tyrosine accumulating cell lines of a dihaploid potato selected by resistance to 5-methyltryptophan

Genotype and composition of culture medium are factors important in the selection for transformed sunflower (Helianthus annum)callus

Increased frost tolerance and amino acid content in leaves, tubers and leaf callus of regenerated hydroxyproline resistant potato clones

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