Progress in phototransduction

Stavenga, Doekele G.; DeGrip, Willem J.; Grip, W.J. de

doi:10.1007/bf00535657

Cited by 4 publications

(5 citation statements)

References 42 publications

(24 reference statements)

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“…As reported above, fly metarhodopsin M emits also in the red range. This is not only found in Musca (this study) and Calliphora (Stavenga, 1983) but also in Drosophila (Stavenga and Stark, unpubl. ;Miller et al, 1984).…”

Section: Discussionsupporting

confidence: 74%

“…The use of in vivo microspectrofluorometry has created a new horizon in the study of the properties of fly photoreceptors. As shown in this report the metarhodopsin of housefly receptors R1-6 displays a distinct red fluorescence, offering a novel opportunity to study the kinetics of visual pigment processes in completely intact, living animals (see also Stavenga, 1983). For example, from the Fig.…”

Section: Discussionmentioning

confidence: 81%

“…Here we demonstrate that housefly metarhodopin fluoresces and, furthermore, we report the emission and excitation spectra of another visual pigment state coined M' (Franceschini et af., 1981a) which is created at extremely bright light intensities. For preliminary accounts see Franceschini and Stavenga (1981) and Stavenga and Franceschini (1981).…”

Section: Introductionmentioning

confidence: 99%

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FLUORESCENCE OF HOUSEFLY VISUAL PIGMENT

Stavenga

Franceschini

Kirschfeld

1984

Photochem & Photobiology

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The fluorescence of housefly photoreceptors was studied in viuo by using the deep pseudopupil technique. Whereas the rhodopsin R490 of the peripheral retinular cells fluoresces negligibly the metarhodopsin M580 fluoresces distinctly in the red. The newly discovered metarhodopsin M' is produced by intense blue light and can be reconverted into rhodopsin by intense long wavelength light. M' also fluoresces in the red; its excitation spectrum and emission spectrum peak at A , , , = 570 and 660 nm respectively. Intense ultraviolet light irreversibly reduces the visual pigment fluorescence as well as the broad band autofluorescencc ( X, , , = 470 nm) originating from non-visual pigments in the fly's eye.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

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FLUORESCENCE OF HOUSEFLY VISUAL PIGMENT

Stavenga

Franceschini

Kirschfeld

1984

Photochem & Photobiology

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“…We performed the cluster analysis of these DGEs and found that among them, the vision-and digestion-related genes were almost all up-regulated and expressed in the CL group (Figure 3B,C), whereas most of the immune system-related genes were up-regulated in the EW group (Figure 3D). Considering that the enrichment analysis results revealed the differential expression of the vision-, digestion-, and immune-related genes in the YFPs under different environment conditions, we combined many literature reviews [19][20][21][22][23][24][25] to screen for these DEGs in the EW and CL groups (Table 4, more information on the genes screened is given in Table S6, KEGG enrichment analysis of the screened genes is shown in Table S7). We performed the cluster analysis of these DGEs and found that among them, the vision-and digestionrelated genes were almost all up-regulated and expressed in the CL group (Figure 3B,C), whereas most of the immune system-related genes were up-regulated in the EW group (Figure 3D).…”

Section: Analysis Of Key Genes and Pathwaysmentioning

confidence: 99%

Blood Transcriptome Analysis Provides Responsive Changes in Gene Expression between Ex Situ and Captive Yangtze Finless Porpoises (Neophocaena asiaeorientalis asiaeorientalis)

Cao,

Yin,

et al. 2023

Fishes

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The Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis, YFP) is an endangered species endemic to the Yangtze River in China, and it is the only freshwater whale in the genus Neophocaena. In terms of protection, three effective conservation strategies exist: in situ conservation, ex situ conservation, and artificial breeding, all of which have been implemented by the Chinese government. Of these, ex situ conservation involves the relocation of Yangtze finless porpoises to semi-natural waters with less human interference, and artificial breeding involves the relocation of Yangtze finless porpoises to a controlled environment that is more strictly managed. To compare and analyze the responsive changes in gene expression of the YFPs between the ex situ and controlled environments, we performed the RNA sequencing of blood tissues from these YFPs. A total of 1201 differentially expressed genes (DEGs) were identified, of which 423 were up-regulated in the ex situ population and 778 were up-regulated in the controlled-environment population. Gene enrichment analysis showed that 1201 DEGs between the ex situ and controlled-environment populations were generally enriched for vision-, digestion- and immune-system-related pathways. Further analysis revealed that several key immune system pathways, such as the chemokine signaling pathway and B cell receptor signal pathway, were activated in the ex situ population. In addition, the key pathways related to vision, including phototransduction and the inflammatory mediator regulation of TRP channels, as well as the pathways related to the digestive system, such as protein digestion and absorption and salivary secretion, were activated in the controlled-environment population. These results suggest that the ex situ populations may respond to complex environmental conditions in semi-natural waters by enhancing their immune function through the increased expression of immune-related genes and that the visual function and protein digestion of the YFPs were improved compared to those of the ex situ population based on the conditions of artificial feeding, such as the higher transparency of the water and regular feeding. This study provides clues for evaluating the adaptability of YFPs to different environments and is a useful reference for future ex situ conservation and artificial breeding.

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“…Photophysical properties of photoreceptors in insects, in turn, have been widely investigated as to both AF emission, light filtering, visual pigments and spectral responses [ 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 ]. Of note, Drosophila is the preferred model of the majority of these studies dealing with photoreceptor mechanisms and visual pigments, taking advantage of the solid knowledge of its biology, genetics, genomics, a standardized rearing, the availability of wild-type flies and well characterized mutants [ 87 ].…”

Section: Autofluorescence In Dipteramentioning

confidence: 99%

Autofluorescent Biomolecules in Diptera: From Structure to Metabolism and Behavior

Croce

Scolari

2022

Molecules

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Light-based phenomena in insects have long attracted researchers’ attention. Surface color distribution patterns are commonly used for taxonomical purposes, while optically-active structures from Coleoptera cuticle or Lepidoptera wings have inspired technological applications, such as biosensors and energy accumulation devices. In Diptera, besides optically-based phenomena, biomolecules able to fluoresce can act as markers of bio-metabolic, structural and behavioral features. Resilin or chitinous compounds, with their respective blue or green-to-red autofluorescence (AF), are commonly related to biomechanical and structural properties, helpful to clarify the mechanisms underlying substrate adhesion of ectoparasites’ leg appendages, or the antennal abilities in tuning sound detection. Metarhodopsin, a red fluorescing photoproduct of rhodopsin, allows to investigate visual mechanisms, whereas NAD(P)H and flavins, commonly relatable to energy metabolism, favor the investigation of sperm vitality. Lipofuscins are AF biomarkers of aging, as well as pteridines, which, similarly to kynurenines, are also exploited in metabolic investigations. Beside the knowledge available in Drosophila melanogaster, a widely used model to study also human disorder and disease mechanisms, here we review optically-based studies in other dipteran species, including mosquitoes and fruit flies, discussing future perspectives for targeted studies with various practical applications, including pest and vector control.

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Progress in phototransduction

Cited by 4 publications

References 42 publications

FLUORESCENCE OF HOUSEFLY VISUAL PIGMENT

FLUORESCENCE OF HOUSEFLY VISUAL PIGMENT

Blood Transcriptome Analysis Provides Responsive Changes in Gene Expression between Ex Situ and Captive Yangtze Finless Porpoises (Neophocaena asiaeorientalis asiaeorientalis)

Autofluorescent Biomolecules in Diptera: From Structure to Metabolism and Behavior

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