Progress in research on phytoestrogens and their effect targets

Shi, Shufang; Yu, Chaoqin

doi:10.3736/jcim20050521

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…Several reports have examined the cholinergic neurons expressing ERα in basal forebrain. Although, as reported, phytoestrogens exhibit a higher affinity for ERβ compared with ERα [34,35], the concentration required for the induction is almost the same with hERβ as with hERα and is much higher than expected from the binding affinity. Moreover, previous study showed that Pueraria mirifica phytoestrogens might partially inhibit the transcription of the ERα gene and then lower the amount of ERα [36].…”

Section: Discussionmentioning

confidence: 67%

Effects of puerarin on cholinergic enzymes in the brain of ovariectomized guinea pigs

Zhang¹,

Chen²,

Ye³

et al. 2013

International Journal of Neuroscience

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Estrogen has beneficial effects on neurodegenerative disorders and cognitive function of postmenopausal women. Puerarin, isolated from Pueraria lobota, has been classified as a phytoestrogen, which can be highly effective against cerebrovascular diseases. In this study, the effects of puerarin on neural cholinergic system in the brain of ovariectomized guinea pigs were studied. The puerarin at the doses used (15 mg/kg body weight (bw)/day and 30 mg/kg bw/day) for 10 days had the estrogenic activity indicated by the attenuation of the reduction of uterine weight induced by ovariectomy. In brain, puerarin treatment increased choline acetyltransferase (ChAT) activity and expression in hippocampus, and increased ChAT immnuopositive signals in septal diagonal region. Puerarin treatment could suppress the increase of acetylcholinesterase expression and activity to the levels of the intact group, although they were not significantly different from those of the ovariectomized animals. Moreover, puerarin decreased the β-amyloid immunopositive staining in hippocampus. In brief, the present study suggests that puerarin prevents the dysfunction of the neuronal cholinergic system and ameliorates the increase of β-amyloid caused by estrogen deficiency.

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Section: Discussionmentioning

confidence: 67%

Effects of puerarin on cholinergic enzymes in the brain of ovariectomized guinea pigs

Zhang¹,

Chen²,

Ye³

et al. 2013

International Journal of Neuroscience

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“…Puerarin could reduce the autocrine of E2 of ectopic endometrium. Researchers reported that E2 played a promoting role in the process of EMT [ 18 , 19 ]. It is commonly believed that E2 could specially combine with ER of endometrial cells and the conformational changes could further promote E2-ER compounds to combine with transcription regulatory proteins.…”

Section: Discussionmentioning

confidence: 99%

The Effects and Possible Mechanisms of Puerarin to Treat Endometriosis Model Rats

Zhao

Zhang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Objective. To explore the effects of puerarin to treat endometriosis (EMT) model rats and the possible regulatory mechanisms. Methods. EMT model rats were surgically induced by autotransplantion of endometrial tissues. The appropriate dosage of puerarin to treat EMT model rats was determined by observing the pathologic morphology of ectopic endometrial tissues and by detecting the levels of estradiol (E2) and prostaglandin E2 (PGE2) of both serum and ectopic endometrial tissues. The related genes and proteins of ectopic endometrial tissues were analyzed by Real-time PCR and immunohistochemistry (IHC) to explore the possible mechanisms. Results. Puerarin could reduce the levels of E2 and PGE2 and prevent the growth of ectopic endometrium tissues by inhibiting the expression of aromatase cytochrome P450 (p450arom) and cyclooxygenase-2 (cox-2); puerarin could adjust the anabolism of E2 by upregulating the expression of 17β-hydroxysteroid-2 (17β-hsd-2) and downregulating the expression of 17β-hydroxysteroid-1 (17β-hsd-1) of the ectopic endometrium tissues; puerarin could increase the expression of ERβ and improve the inflammatory microenvironment of EMT model rats. Conclusions. Our data suggest that puerarin has a therapeutic effect on EMT model rats and could be a potential therapeutic agent for the treatment of EMT in clinic.

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“…CA with hepatoprotective properties is derived from ASH and is considered as one of the major metabolite (Cai et al, 2020). Bile acids, such as taurodeoxycholic acid (TA) and glycohyodeoxycholic acid (GA), are the main active metabolites of PFS, which have anti-inflammatory, liver protection, cholagogic and detoxification effects (Yan et al, 2017;Shi et al, 2018;Chen et al, 2021). Moreover, TA and GA was identified in the HGT characteristic chromatogram described above (Figure 3 and Supplementary Table S5).…”

Section: Identification Of Specific Secondary Metabolites Of Botanica...mentioning

confidence: 99%

A funnel-type stepwise filtering strategy for identification of potential Q-markers of traditional Chinese medicine formulas

et al. 2023

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Quality marker (Q-marker) serves as an important driver for the standardization of quality control in traditional Chinese medicine (TCM) formulas. However, it is still challenging to discover comprehensive and representative Q-markers. This study aimed to identify Q-markers of Hugan tablet (HGT), a famous TCM formula with ideal clinical effects in liver diseases. Here, we proposed a funnel-type stepwise filtering strategy that integrated secondary metabolites characterization, characteristic chromatogram, quantitative analysis, literature mining, biotransformation rules and network analysis. Firstly, the strategy of “secondary metabolites-botanical drugs-TCM formula” was applied to comprehensively identify the secondary metabolites of HGT. Then, the secondary metabolites with specificity and measurability in each botanical drug were identified by HPLC characteristic chromatogram, biosynthesis pathway and quantitative analysis. Based on literature mining, the effectiveness of botanical metabolites that met the above conditions was evaluated. Furthermore, the metabolism of the above metabolites in vivo was studied to reveal their biotransformation forms, which were used for network analysis. At last, according to biotransformation rules of the prototype drugs in vivo, the secondary metabolites were traced and preliminarily chosen as Q-markers. As a result, 128 plant secondary metabolites were identified in HGT, and 11 specific plant secondary metabolites were screened out. Then, the content of specific plant secondary metabolites in 15 batches of HGT was determined, which confirmed their measurability. And the results of literature mining showed that eight secondary metabolites had therapeutic effects in treating liver disease at the in vivo level, and three secondary metabolites inhibited liver disease-related indicators at the in vitro level. After that, 26 compounds absorbed into the blood (11 specific plant metabolites and their 15 metabolites in vivo) were detected in rats. Moreover, 14 compounds, including prototype components and their metabolites, were selected as Q-marker candidates by the “TCM formula-botanical drugs-compounds-targets-pathways” network. Finally, 9 plant secondary metabolites were defined as comprehensive and representative Q-markers. Our study not only provides a scientific basis for the improvement and secondary development of the quality standard of HGT, but also proposes a reference method for discovering and identifying Q-markers of TCM preparations.

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Progress in research on phytoestrogens and their effect targets

Cited by 5 publications

References 17 publications

Effects of puerarin on cholinergic enzymes in the brain of ovariectomized guinea pigs

Effects of puerarin on cholinergic enzymes in the brain of ovariectomized guinea pigs

The Effects and Possible Mechanisms of Puerarin to Treat Endometriosis Model Rats

A funnel-type stepwise filtering strategy for identification of potential Q-markers of traditional Chinese medicine formulas

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