Progressive aggregation of Tomato yellow leaf curl virus coat protein in systemically infected tomato plants, susceptible and resistant to the virus

Gorovits, Rena; Moshe, Adi; Kolot, Mikhail; Sobol, Iris; Czosnek, Henryk

doi:10.1016/j.virusres.2012.09.017

Cited by 39 publications

(98 citation statements)

References 58 publications

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“…4b). In TYLCV susceptible tomatoes, the virus amount constantly increased until it reached a plateau maintained for up to 60 dpi (Gorovits et al 2013a;Anfoka et al 2016). In infected Hsp90-silenced tomatoes, despite the plant decline being partially suppressed by TYLCV infection, virus CP and DNA accumulated at least until 35 dpi ; Fig.…”

Section: Accumulation Of Tylcv In Tomatoes During Changing Stress Resmentioning

confidence: 89%

“…Western blotting was performed as described (Gorovits et al 2013a, b) using antibodies against the TYLCV CP (raised in our laboratory, Gorovits et al 2013a), HSP70 (AS08371) and ubiquitin (AS08307) (Agrisera, Sweden), proteasome 20S alpha-beta (ab22673) (Abcam, UK), HSFA2 (a gift from Prof. K.D. Scharf; described in Scharf et al 1998), and HSP17 (a gift of Prof. A. Grover; described in PhD thesis of Agarwal 2002).…”

Section: Immunodetection Of Viral and Plant Proteinsmentioning

confidence: 99%

“…DNA, RNA extractions and qPCR/qRT-PCR DNA was prepared from 100-mg leaf tissues as described (Gorovits et al 2013a). For qPCR, 50 ng DNA was used in triplicate for each DNA sample.…”

Section: Immunodetection Of Viral and Plant Proteinsmentioning

confidence: 99%

“…During infection of TYLCV-susceptible tomatoes, TYLCV DNA and CP accumulate steadily until they reach stable levels up to 60 dpi (Gorovits et al 2013a). Virus CP and DNA were monitored in tomatoes with silenced Hsp90, agroinfiltrated with various PVX constructs (Fig.…”

Section: Accumulation Of Tylcv In Tomatoes During Changing Stress Resmentioning

confidence: 99%

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The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation

Gorovits

Moshe

Amrani

et al. 2017

Cell Stress and Chaperones

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Tomato yellow leaf curl virus (TYLCV) is a begomovirus infecting tomato plants worldwide. TYLCV needs a healthy host environment to ensure a successful infection cycle for long periods. Hence, TYLCV restrains its destructive effect and induces neither a hypersensitive response nor cell death in infected tomatoes. On the contrary, TYLCV counteracts cell death induced by other factors, such as inactivation of HSP90 functionality. Suppression of plant death is associated with the inhibition of the ubiquitin 26S proteasome degradation and with a deactivation of the heat shock transcription factor HSFA2 pathways (including decreased HSP17 levels). The goal of the current study was to find if the individual TYLCV genes were capable of suppressing HSP90-dependent death and HSFA2 deactivation. The expression of C2 (C3 and CP to a lesser extent) caused a decrease in the severity of death phenotypes, while the expression of V2 (C1 and C4 to a lesser extent) strengthened cell death. However, C2 or V2 markedly affected stress response under conditions of viral infection. The downregulation of HSFA2 signaling, initiated by the expression of C1 and V2, was detected in the absence of virus infection, but was enhanced in infected plants, while CP and C4 mitigated HSFA2 levels only in the infected tomatoes. The dependence of analyzed plant stress response suppression on the interaction of the expressed genes with the environment created by the whole virus infection was more pronounced than on the expression of individual TYLCV genes.

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Section: Accumulation Of Tylcv In Tomatoes During Changing Stress Resmentioning

confidence: 89%

Section: Immunodetection Of Viral and Plant Proteinsmentioning

confidence: 99%

“…DNA, RNA extractions and qPCR/qRT-PCR DNA was prepared from 100-mg leaf tissues as described (Gorovits et al 2013a). For qPCR, 50 ng DNA was used in triplicate for each DNA sample.…”

Section: Immunodetection Of Viral and Plant Proteinsmentioning

confidence: 99%

Section: Accumulation Of Tylcv In Tomatoes During Changing Stress Resmentioning

confidence: 99%

See 2 more Smart Citations

The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation

Gorovits

Moshe

Amrani

et al. 2017

Cell Stress and Chaperones

Self Cite

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show abstract

“…AC3 and AC4) (Navot et al, 1991). AV1 encodes coat protein (CP) and is likely involved in virus nucleo-cytoplasmic shuttling; its siRNA targeted against CP can confer resistance to TYLCV in transgenic tomato plants (Gorovits et al, 2013;Zrachya et al, 2007b). AV2 participates in virus infection and suppresses RNA silencing (Zrachya et al, 2007a).…”

Section: Introductionmentioning

confidence: 99%

Transient expression of siRNA targeted against the TYLCV AV1, AC1 and AC3 genes for high resistance in tomato

Sun

Kang

Xing

et al. 2014

Scientia Horticulturae

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Degradation mechanisms of the Tomato yellow leaf curl virus coat protein following inoculation of tomato plants by the whitefly Bemisia tabaci

Gorovits

Moshe

Ghanim³

et al. 2014

Pest Management Science

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Progressive aggregation of Tomato yellow leaf curl virus coat protein in systemically infected tomato plants, susceptible and resistant to the virus

Cited by 39 publications

References 58 publications

The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation

The six Tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation

Transient expression of siRNA targeted against the TYLCV AV1, AC1 and AC3 genes for high resistance in tomato

Degradation mechanisms of the Tomato yellow leaf curl virus coat protein following inoculation of tomato plants by the whitefly Bemisia tabaci

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