Progressive multifocal leukoencephalopathy diagnosed by amplification of JC virus-specific DNA from cerebrospinal fluid

Weber, Thomas K.; Turner, Rodney W.; Frye, Stephan A.; Lüke, Wolfgang; Kretzschmar, Hans A.; Lüer, W.; Hunsmann, Gerhard

doi:10.1097/00002030-199401000-00008

Cited by 53 publications

(24 citation statements)

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“…1). The detection threshold values of our system were relatively similar or even better than those of previous reports (1 1,12,14). The results of nested PCRanalysis are summarized in Table 2 together with the pathological diagnosis.…”

Section: Resultssupporting

confidence: 83%

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Detection of Toxoplasma gondii, Epstein-Barr Virus, and JC Virus DNAs in the Cerebrospinal Fluid in Acquired Immunodeficiency Syndrome Patients with Focal Central Nervous System Comlications.

et al. 1999

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Object Toxoplasmic encephalitis (TE), primary central nervous system lymphoma (PCNSL) and progressive multifocal leukoencephalopathy (PML) are major central nervous system (CNS) diseases in patients with acquired immunodeficiency syndrome (AIDS). Weassessed the diagnostic value of polymerase chain reaction (PCR) in the detection of DNAsof Toxoplasma gondii {T. gondii), Epstein-Barr virus (EBV) and JC virus (JCV) in the cerebrospinal fluid (CSF). Methods Wecompared the PCRresults with those of pathological findings at autopsy. Patients or Materials The present study included 23 autopsies representing those in whomCSF samples were obtained before death while the patient was hospitalized or at autopsy. Results The threshold levels for PCR detection were 4 tachyzoites of T. gondii, 5-15 genomes of EBVand 10 genomes of JCV. Weidentified T. gondii DNAin 4 out of5 autopsy-defined cases ofTE, EBVDNAin 5 out of5 cases with PCNSL, and JCV DNAin 2 out of2 cases with PML.The specificity ofPCR was 100% in TE, 78%in PCNSL, and 100% in PML. Conclusion Although the number of cases was relatively small in this study, PCRcorrectly identified T. gondii DNAin those cases in which PMLor PCNSL was the sole clinical diagnosis. Our results indicate that PCRexamination of CSFis a clinically useful tool for the diagnosis of focal brain lesions in patients with AIDS. (Internal Medicine 38: 556-562, 1999)

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Section: Resultssupporting

confidence: 83%

“…The large T antigen gene of JCV was amplified as described previously (14). The primers and conditions used in the assay are listed in Table 1.…”

Section: Jcv (Jcv-nested Pcr)mentioning

confidence: 99%

Detection of Toxoplasma gondii, Epstein-Barr Virus, and JC Virus DNAs in the Cerebrospinal Fluid in Acquired Immunodeficiency Syndrome Patients with Focal Central Nervous System Comlications.

et al. 1999

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“…JCPyV detected in 12 urine clinical samples that previously tested positive using a nested PCR to amplify the T antigen region of the DNA genome (Weber et al 1994 …”

Section: Clinical Samplesmentioning

confidence: 99%

Molecular detection, quantification and characterization of human polyomavirus JC from waste water in Rio De Janeiro, Brazil

Fumian¹,

Guimarães

Vaz³

et al. 2010

Journal of Water and Health

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Polyomavirus JC (JCPyV) is largely excreted by the human population through the urinary route and has been recognized as a potential viral marker for human waste contamination. This study aims to investigate the dissemination of JCPyV in waste water from a sewage treatment plant (STP) located in Rio de Janeiro, Brazil, and to describe the prevalence of JCPyV subtypes currently present in this population. Raw and treated sewage samples were collected bimonthly during one year, and examined for the presence of JCPyV using nested polymerase chain reaction (nPCR) and quantitative real time PCR (qPCR). JCPyV was detected by nPCR in 96% and 43% of raw and treated sewage samples, respectively. The concentration of JCPyV present in the samples ranged from 1.2 £ 10 3 to 3.2 £ 10 5 and 2.6 £ 10 2 to 6.2 £ 10 3 genome copies per 2 ml of concentrated raw and treated sewage sample, respectively. The strains were characterized and the obtained nucleotide sequences indicated that the detected JCPyV strains clustered with subtypes of East African, West African and European origin. To our knowledge, this is the first study describing the incidence and diversity of JCPyV strains in raw and treated sewage in Brazil.

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“…Confirmation of PML diagnosis at this time is possible by positive in situ hybridization of fixed brain biopsy tissue with a JCV-specific DNA probe and/or PCR amplification of viral sequences from cerebrospinal fluid (CSF) [93][94][95]. Previously cited tissues from brain-biopsy-positive PML patients generally contain JCV DNA, as do some of the same tissues from non-PML patients.…”

Section: Cloning Techniquementioning

confidence: 99%

Viral variant nucleotide sequences help expose leukocytic positioning in the JC virus pathway to the CNS

Jensen

Major

1999

Journal of Leukocyte Biology

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Progressive multifocal leukoencephalopathy diagnosed by amplification of JC virus-specific DNA from cerebrospinal fluid

Cited by 53 publications

References 0 publications

Detection of Toxoplasma gondii, Epstein-Barr Virus, and JC Virus DNAs in the Cerebrospinal Fluid in Acquired Immunodeficiency Syndrome Patients with Focal Central Nervous System Comlications.

Detection of Toxoplasma gondii, Epstein-Barr Virus, and JC Virus DNAs in the Cerebrospinal Fluid in Acquired Immunodeficiency Syndrome Patients with Focal Central Nervous System Comlications.

Molecular detection, quantification and characterization of human polyomavirus JC from waste water in Rio De Janeiro, Brazil

Viral variant nucleotide sequences help expose leukocytic positioning in the JC virus pathway to the CNS

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