Prokaryotic expression and identification of scavenger receptor B2

Abstract

There is still no effective clinical antiviral drug against human enterovirus 71 (EV71) infection, which causes hand, foot and mouth disease (HFMD) in children. Scavenger receptor class B member 2 (SCARB2) is an important receptor of EV71 as it plays a vital role in the early steps of viral infection. In this study, recombinant SCARB2 protein was expressed and purified in a prokaryotic expression system, and was identified by western blot with a monoclonal antibody and mass spectrometry analysis. Detection of the sera from mice immunized with the recombinant SCARB2 protein using ELISA and western blot showed good immunogenicity of the recombinant protein. Furthermore, in the neutralization test cytopathic effect was significantly decreased when EV71 was incubated with the immune sera before infection. In summary, the SCARB2 protein was expressed successfully, and the immune sera showed obvious antiviral effect against EV71. This study provides useful information about the interaction mechanism between SCARB2 and EV71, and is also helpful for further clinical treatment research of HFMD.

Inhibition of Enterovirus 71 by Selenium Nanoparticles Loaded with siRNA through Bax Signaling Pathways

“…SK-N-SH cells transfected with Se@PEI@siRNA were infected with EV71 for 48 h. EV71 VP1, Bax, and β-actin monoclonal antibodies were used for incubation. , …”

“…SK-N-SH cells transfected with Se@PEI@ siRNA were infected with EV71 for 48 h. EV71 VP1, Bax, and β-actin monoclonal antibodies were used for incubation. 31,32 Statistical Analysis. GraphPad Prism 7.0 software was used for data analysis.…”

The inhibition of enterovirus 71 induced apoptosis by Durvillaea antarctica through P53 and STAT1 signaling pathway

“…Enterovirus 71 (EV71) is a major etiological agent of hand, foot, and mouth disease, which affects infants and children 1–3 . Most of these infections are mild symptoms, such as fever, rash, and sores on the skin of hands, feet, and mouth.…”

“…Enterovirus 71 (EV71) is a major etiological agent of hand, foot, and mouth disease, which affects infants and children. [1][2][3] Most of these infections are mild symptoms, such as fever, rash, and sores on the skin of hands, feet, and mouth. However, some patients show severe central nervous system damage, such as aseptic meningitis, brainstem encephalitis, and acute flaccid paralysis.…”