Prolactin is a component of the human synovial liquid and modulates the growth and chondrogenic differentiation of bone marrow-derived mesenchymal stem cells

Ogueta, Samuel; Muñoz, Jaime; Obregón, Eva; Delgado-Baeza, Emilio; García–Ruiz, Josefa P.

doi:10.1016/s0303-7207(02)00013-8

Cited by 84 publications

(86 citation statements)

References 39 publications

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“…The hMSCs used here were isolated from human bone marrow samples from healthy anonymous donors provided by the hematology services of Hospital La Princesa, the Jiménez-Díaz Foundation, and the Biobank of the University of Málaga, and expanded as described previously [44,45] with the approval of the ethical committees of the institutions.…”

Section: Cell Culture Processes and Imaging Techniquesmentioning

confidence: 99%

“…Cell culture mediums were prepared by the research services of the "Severo Ochoa" Molecular Biology Center (CSIC-UAM) as previously detailed [44,45] with some modifications. For the preparation of the hMSC conditioned medium, we used 8-10 p100 culture plates at 80% confluence for each batch, following previous successful experiences [36].…”

Section: Cell Culture Processes and Imaging Techniquesmentioning

confidence: 99%

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Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility

2015

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This paper presents the complete development and experimental validation of a microsystem designed to systematically assess the impact of surface topography on cell adhesion and dynamics. The microsystem includes two pools for culturing cells and for including chemicals. These pools are connected by several channels that have different microtextures, along which the cells crawl from one well to another. The impact of channel surface topography on cell performance, as well as the influence of other relevant factors, can therefore be assessed. The microsystem stands out for its being able to precisely define the surface topographies from the design stage and also has the advantage of including the different textures under study in a single device. Validation has been carried out by culturing human mesenchymal stem cells (hMSCs) on the microsystem pre-treated with a coating of hMSC conditioned medium (CM) produced by these cells. The impact of surface topography on cell adhesion, motility, and velocity has been quantified, and the relevance of using a coating of hMSC-CM for these kinds of studies has been analyzed. Main results, current challenges, and future proposals based on the use of the proposed microsystem as an experimental resource for studying cell mechanobiology are also presented.

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Section: Cell Culture Processes and Imaging Techniquesmentioning

confidence: 99%

Section: Cell Culture Processes and Imaging Techniquesmentioning

confidence: 99%

Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility

2015

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“…The hMSCs used were isolated from 1 to 2 ml from bone marrow-derived samples of healthy donors and were provided by Hospital La Princesa, the Jimenez-Diaz Foundation and Malaga University Biobank. Cells were isolated and culture expanded following processes described in previous research [17][18][19] with slight modifications. In short, culture media DMEMlow glucose (LG), 0.25% trypsin-EDTA and phosphate buffered saline (PBS) were prepared by the Research Service of the Molecular Biology Centre "Severo Ochoa."…”

Section: Human Mesenchymal Stem Cells (Hmscs) Culturementioning

confidence: 99%

Monolithic 3D labs- and organs-on-chips obtained by lithography-based ceramic manufacture

Lantada

Romero

Schwentenwein³

et al. 2017

Int J Adv Manuf Technol

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In this study, we present a novel approach for the design and development of three-dimensional monolithic ceramic microsystems with complex geometries and with potential applications in the biomedical field, mainly linked to labson-chips and organs-on-chips. The microsystem object of study stands out for its having a complex three-dimensional geometry, for being obtained as a single integrated element, hence reducing components, preventing leakage and avoiding post-processes, and for having a cantilever porous ceramic membrane aimed at separating cell culture chambers at different levels, which imitates the typical configuration of transwell assays. The design has been performed taking account of the special features of the manufacturing technology and includes ad hoc incorporated supporting elements, which do not affect overall performance, for avoiding collapse of the cantilever ceramic membrane during debinding and sintering. The manufacture of the complex three-dimensional microsystem has been accomplished by means of lithography-based ceramic manufacture, the additive manufacturing technology which currently provides the most appealing compromises between overall part size and precision when working with ceramic materials. The microsystem obtained provides one of the most remarkable examples of monolithic bio-microsystems and, to our knowledge, a step forward in the field of ceramic microsystems with complex geometries for lab-on-chip and organ-on-chip applications. Cell culture results help to highlight the potential of the proposed approach and the adequacy of using ceramic materials for biological applications and for interacting at a cellular level.

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“…The hMSCs used in this work were isolated in a Percoll gradient from 1 or 2 ml of human bone marrow samples from anonymous healthy donors and provided by hematology services of the Hospital La Princesa, the Jiménez Díaz Foundation and the University Biobank of Málaga, and expanded as described previously [42,43]. Cells were plated and incubated using DMEM L plus 10% FBS of selected batches.…”

Section: Cell Culture Process For In Vitro Validationmentioning

confidence: 99%

Composite scaffolds for osteochondral repair obtained by combination of additive manufacturing, leaching processes and hMSC-CM functionalization

Lantada

Iniesta

García–Ruiz

2016

Materials Science and Engineering: C

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A b s t r a c tArticular repair is a relevant and challenging area for the emerging fields of tissue engineering and biofabrication. The need of significant gradients of properties, for the promotion of osteochondral repair, has led to the develop ment of several families of composite biomaterials and scaffolds, using different effective approaches, although a perfect solution has not yet been found. In this study we present the design, modeling, rapid manufacturing and in vitro testing of a composite scaffold aimed at osteochondral repair. The presented composite scaffold stands out for having a functional gradient of density and stiffness in the bony phase, obtained in titanium by means of computer aided design combined with additive manufacture using selective laser sintering. The chondral phase is obtained by sugar leaching, using a PDMS matrix and sugar as porogen, and is joined to the bony phase during the polymerization of PDMS, therefore avoiding the use of supporting adhesives or additional intermediate layers. The mechanical performance of the construct is biomimetic and the stiffness values of the bony and chondral phases can be tuned to the desired applications, by means of controlled modifications of different parameters. A human mesenchymal stem cell (h MSC) conditioned medium (CM) is used for improving scaffold response. Cell culture results provide relevant information regarding the viability of the composite scaffolds used.

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Prolactin is a component of the human synovial liquid and modulates the growth and chondrogenic differentiation of bone marrow-derived mesenchymal stem cells

Cited by 84 publications

References 39 publications

Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility

Multi-Channeled Polymeric Microsystem for Studying the Impact of Surface Topography on Cell Adhesion and Motility

Monolithic 3D labs- and organs-on-chips obtained by lithography-based ceramic manufacture

Composite scaffolds for osteochondral repair obtained by combination of additive manufacturing, leaching processes and hMSC-CM functionalization

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