Prolidase – A protein with many faces

Wilk, P.; Wator, E.; Weiss, M.S.

doi:10.1016/j.biochi.2020.09.017

Cited by 21 publications

(12 citation statements)

References 121 publications

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“…This indicates that prolidase was activated in response to Le treatment ( Supplementary Table 2 ). According to Wilk et al (2021) , prolidase is postulated as being involved in nutrition acquisition, and antitoxin defense in prokaryotes. Prolidase has been shown to contribute to osmoregulation by releasing free proline ( Zaprasis et al, 2013 ), and prolidase variations have been linked to antibiotic resistance ( Wilk et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…According to Wilk et al (2021) , prolidase is postulated as being involved in nutrition acquisition, and antitoxin defense in prokaryotes. Prolidase has been shown to contribute to osmoregulation by releasing free proline ( Zaprasis et al, 2013 ), and prolidase variations have been linked to antibiotic resistance ( Wilk et al, 2021 ). Prolidases are believed to have a broad antitoxin protective role due to their capacity to hydrolyse organophosphorus chemicals ( Štěpánková et al, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

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Enzymatic and proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against Botrytis cinerea (causative pathogen of gray mold)

et al. 2023

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IntroductionEssential oils (EOs) have been demonstrated as efficacious against B. cinerea. However, the underpinning enzymatic and proteomic mechanism for these inhibitory effects is not entirely clear.MethodsThus, this study examined the effects of lemon (Le) and lemongrass (Lg) EOs (individually and in combination) against B. cinerea based on enzymatic and proteomic analyses. Proteomics data are available via ProteomeXchange with identifier PXD038894.Results and discussionBoth EOs (individually and in combination) displayed abilities to induce scavenging as observed with the reduction of H2O2. Measured malondialdehyde (MDA) and superoxide dismutase (SOD) activity were increased in all EOs treated B. cinerea mycelia compared to the control. Ascorbate peroxidase (APX) activity was highest in Lg treated B. cinerea (206% increase), followed by combined (Le + Lg) treatment with 73% compared to the untreated control. Based on GC-MS analysis, the number of volatile compounds identified in lemon and lemongrass EOs were 7 and 10, respectively. Major chemical constituent of lemon EO was d-limonene (71%), while lemongrass EO was a-citral (50.1%). Based on the interrogated LC-MS data, 42 distinct proteins were identified, and 13 of these proteins were unique with 1, 8, and 4 found in Le-, Lg-, and (Le + Lg) EOs treated B. cinerea, respectively, and none in control. Overall, 72% of identified proteins were localized within cellular anatomical entity, and 28% in protein-complexes. Proteins involved in translation initiation, antioxidant activity, protein macromolecule adaptor activity and microtubule motor activity were only identified in the Lg and (Le + Lg) EOs treated B. cinerea mycelia, which was consistent with their APX activities.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Enzymatic and proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against Botrytis cinerea (causative pathogen of gray mold)

et al. 2023

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“…Prolidase is a member of the MMP family. It is capable of degrading imidopeptides and imidotripeptides with C‐terminal proline and hydroxyproline from ECM collagen 18 . Vural et al 12 found that placental prolidase enzyme activity was higher among women with early pregnancy loss than among women without early pregnancy loss.…”

Section: Discussionmentioning

confidence: 99%

“…Scatter plot of prolidase and total oxidant status (TOS) levels in the patient and control groups F I G U R E 3 Scatter plot of vitamin E and total oxidant status (TOS) levels in the patient and control groups and hydroxyproline from ECM collagen. 18 Vural et al 12 found that placental prolidase enzyme activity was higher among women with early pregnancy loss than among women without early pregnancy loss. Also, Soydinc et al 19 demonstrated that vaginal fluid levels of prolidase were significantly higher in a group of women with preterm prelabor rupture of membranes than in a control group.…”

Section: Variablesmentioning

confidence: 99%

Role of prolidase activity and oxidative stress biomarkers in unexplained infertility

Isbilen

Kulaksızoğlu

Kirmizioglu

et al. 2021

Intl J Gynecology & Obste

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Objective: Our aim was to explore the significance of serum prolidase enzyme activity and oxidative stress in women with unexplained infertility (UEI). Methods:In this case-control study (n = 160; 86 cases; 74 controls) prolidase enzyme activity and total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), and vitamin E were measured in plasma using enzyme-linked immunosorbent assays.Results: Prolidase enzyme activity and TAS levels were particularly higher in the patient group (P = 0.013, P = 0.001, respectively). Decreased OSI levels were detected in the patient group (P = 0.001). There was a positive relationship of prolidase with vitamin E in both patient and control groups (r = 0.892, P = 0.001, and r = 0.659, P = 0.001, respectively). A positive, but weak, relationship was identified between prolidase activity and TOS levels and also between vitamin E and TOS levels in the UEI group (r = 0.265, P = 0.049, and r = 0.288, P = 0.014, respectively). No association was found between prolidase and TOS levels or between vitamin E and TOS levels in the control group (r = 0.0097, P = 0.527, and r = 0.085, P = 0.610, respectively). Conclusion:Our results showed an association between serum prolidase activity and oxidative stress in UEI patients. Further studies including greater groups are required to show the role of reactive oxygen species in UEI.

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“…Prolidase enzyme is a member of the matrix metalloproteinase family and is present in many tissues including erythrocytes, leukocytes, fibroblasts, and plasma as well as brain, intestinal mucosa, liver, kidney, uterus, thymus, and heart (Zanaboni et al , 1994; Wilk et al , 2021; Abdullah et al , 2022). Prolidase enzyme in the brain takes part in the regulation of proline levels, which is considered a neurotransmitter in the central nervous system (Bolu et al , 2021).…”

Section: Introductionmentioning

confidence: 99%

Assessing the relationship between antipsychotic drug use and prolidase enzyme activity and oxidative stress in schizophrenia patients: a case-control study

Yazla

Çetin

Kayadibi

2023

International Clinical Psychopharmacology

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Background The relationship between proline, its association with oxidative stress, and its connection to schizophrenia is a subject that has not been sufficiently investigated. Objective The aim of this study is to evaluate the possible effects of atypical and combined (typical and atypical) antipsychotic use on serum prolidase enzyme activity (SPEA) and serum oxidative stress parameters, and to assess the relationship between SPEA and oxidative stress in patients with schizophrenia. Methods A total of 57 patients with schizophrenia, of which 34 were using atypical (AAPG) and 23 were using combined (typical and atypical) (CAPG) antipsychotic therapy, and 28 healthy volunteers (control group) were included in this case-control study. Results SPEA levels of AAPG and CAPG were significantly lower than that of control group (P = 0.003). The oxidative stress index (OSI) value of AAPG was significantly higher than the other two groups (P = 0.001). SPEA (<1860 U/l) and OSI (≥0.54) could discriminate schizophrenia patients with antipsychotic therapy from control groups (P = 0.001 and P = 0.007, respectively). Lower SPEA levels were associated with antipsychotic use (P = 0.007). Conclusion The SPEA values of patients with schizophrenia on antipsychotics were significantly lower compared to controls. OSI values were significantly higher in atypical antipsychotic recipients compared to those on combined antipsychotics and healthy controls.

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Prolidase – A protein with many faces

Cited by 21 publications

References 121 publications

Enzymatic and proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against Botrytis cinerea (causative pathogen of gray mold)

Enzymatic and proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against Botrytis cinerea (causative pathogen of gray mold)

Role of prolidase activity and oxidative stress biomarkers in unexplained infertility

Assessing the relationship between antipsychotic drug use and prolidase enzyme activity and oxidative stress in schizophrenia patients: a case-control study

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