Proliferating Cell Nuclear Antigen in Normal and Regenerating Rat Livers

Tanno, Munehiko; Taguchi, Takahiko

doi:10.1006/exmp.1999.2281

Cited by 15 publications

(11 citation statements)

References 33 publications

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“…The above findings showed that PDRN treatment of hepatectomized rat accelerated liver regeneration through rapid cell proliferation. Our results are related to the previous reports of the effects of PDRN on other diseases (Tanno & Taguchi 1999;Rubegni et al 2001;Montesinos et al 2002;Arena et al 2012).…”

Section: Discussionsupporting

confidence: 91%

Polydeoxyribonucleotide administration promotes the expression of proliferating cell nuclear antigen during liver regeneration in rats

Yoon

Kim

Han

et al. 2015

Animal Cells and Systems

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Polydeoxyribonucleotide (PDRN) is an agonist of the A2A adenosine receptors. We evaluated the effects of PDRN on liver regeneration induced by partial hepatectomy (PH) in rats, focusing on cell proliferation. Proliferating cell nuclear antigen (PCNA) as an indicator of cell proliferation was targeted to determine whether PDRN treatment could promote proliferation of hepatic cells in regenerating liver. Male Sprague-Dawley rats were divided into three groups: normal group (n = 3) without PH (sham operated), control groups (n = 12) with saline injection after PH, and experimental groups (n = 12) with i.p. injection of 8 mg/kg of PDRN immediately after PH. Light microscopically, liver regeneration induced by PH involved proliferation of various hepatic cells including parenchymal cells and Kupffer cells. The disintegration (1 or 2 days after PH) and remodeling (4 days after PH) of hepatic plate and increase of sinusoids (6 days after PH) were sequentially observed. These regenerative processes occurred relatively earlier in the experimental groups. By western blotting, the expression of PCNA increased in the early stage, reached its peak at 1 and 2 days after PH, and decreased thereafter. By immunohistochemistry, the intensities of PCNA staining in the experimental groups were more obvious at various days after PH. By electron microscopy using immunogold labeling for PCNA, the gold particles were largely observed in heterochromatin and nucleolus in hepatic cells. The results of immunochemistry and western blotting for PCNA showed the similar pattern in most groups. These findings suggest that PDRN treatment to hepatectomized rat could accelerate liver regeneration through rapid cell proliferation.

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Section: Discussionsupporting

confidence: 91%

Polydeoxyribonucleotide administration promotes the expression of proliferating cell nuclear antigen during liver regeneration in rats

Yoon

Kim

Han

et al. 2015

Animal Cells and Systems

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“…Expression of this protein is a sign of S-phase of the cell cycle, and this protein can be used as a proliferation marker because it represents the proliferative fraction of cells [4]. Positive cells with PCNA antibodies are very few in normal liver; however, they are found to be increased at 24 and 48 h after resection [5].…”

Section: Introductionmentioning

confidence: 98%

The Effect of Amrinone on Liver Regeneration in Experimental Hepatic Resection Model1

Akçan

Küçük

et al. 2006

Journal of Surgical Research

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“…PCNA is known as a docking station that encircles DNA and coordinates multiple genetic functions during DNA replication and repair [17 -20]. But there is a substantial pool of nucleoplasmic PCNA that may work even without interacting with DNA [4,21,22]. The growth-related properties of PCNA, as well as its physicochemical properties (pI, mass, antigenicity) have been well conserved during evolution [23], suggesting that it plays a vital role in cellular metabolism.…”

Section: Introductionmentioning

confidence: 99%

Proliferating cell nuclear antigen: a proteomics view

Naryzhny

2008

Cell. Mol. Life Sci.

204

167

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Proliferating cell nuclear antigen (PCNA), a cell cycle marker protein, is well known as a DNA sliding clamp for DNA polymerase delta and as an essential component for eukaryotic chromosomal DNA replication and repair. Due to its mobility inside nuclei, PCNA is dynamically presented in a soluble or chromatin-associated form. The heterogeneity and specific modifications of PCNA may reflect its multiple functions and the presence of many binding partners in the cell. The recent proteomics approaches applied to characterizing PCNA interactions revealed multiple PCNA partners with a wide spectrum of activity and unveiled the possible existence of new PCNA functions. Since more than 100 PCNA-interacting proteins and several PCNA modifications have already been reported, a proteomics point of view seems exactly suitable to better understand the role of PCNA in cellular functions.

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Proliferating Cell Nuclear Antigen in Normal and Regenerating Rat Livers

Cited by 15 publications

References 33 publications

Polydeoxyribonucleotide administration promotes the expression of proliferating cell nuclear antigen during liver regeneration in rats

Polydeoxyribonucleotide administration promotes the expression of proliferating cell nuclear antigen during liver regeneration in rats

The Effect of Amrinone on Liver Regeneration in Experimental Hepatic Resection Model1

Proliferating cell nuclear antigen: a proteomics view

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