Proliferation and differentiation into endothelial cells of human bone marrow mesenchymal stem cells (MSCs) on poly DL-lactic-co-glycolic acid (PLGA) films

Yue, Huimin; Zhang, Lei; Wang, Yunfang; Feng, Liang; Guan, Lina; Li, Shaoqing; Yan, Fang; Nan, Xue; Bai, Cixian; Lin, Feng; Yan, Yongnian; Pei, Xuetao

doi:10.1007/s11434-006-1328-5

Cited by 13 publications

(3 citation statements)

References 8 publications

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“…Stem cells are a population possessing (1) self-renewal capacity, (2) long-term viability and (3) multilineage potential. The multilineage potential of embryonic stem cells and adult stem cells from the bone marrow has been characterized extensively [3,4] . During embryonic development, stem cells from the blastocyst give rise to cell progenies that seem to become progressively restricted in their phenotypic potential to generate mature tissues [5] .…”

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confidence: 99%

Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture

et al. 2007

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To investigate the biological character of human adipose-derived adult stem cells (hADAS cells) when cultured in vitro and the relationship between hADAS cell's replication activity and the donor's age factor, and to assess the stem cells as a new source for tissue engineering. hADAS cells are isolated from human adipose tissue of different age groups (from adolescents to olds: <20 years old, 21-40 years old, 41-60 years old and >61 years old groups). The protein markers (CD29, CD34, CD44, CD45, CD49d, HLA-DR, CD106) of hADAS cells were detected by flow cytometry (FCM) to identify the stem cell, and the cell cycle was examined for P20 hADAS cells to evaluate the safety of the subculture in vitro. The generative activity of hADAS cells in different age groups was also examined by MTT method. The formula "TD = t x log2/logNt - logN0" was used to get the time doubling (TD) of the cells. The results showed that the cells kept heredity stabilization by chromosome analysis for at least 20 passages. The TD of these cells increased progressively by ageing, and the TD of the <20 years old group was lower than that of the >61 years old group (statistical analysis of variance (ANOVA), P=0.002, P<0.05). These findings suggested that a higher level of hADAS cells replication activity was found in the younger donators, and they represent novel and valuable seed cells for studies of tissue engineering.

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confidence: 99%

Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture

et al. 2007

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“…Since the first use of synthetic biodegradable sutures in the latter half of the 1960s, 1 biodegradable polymers prepared from poly(lactic acid) (PLA), poly(glycolic acid) (PGA), and other degradable poly(a-hydroxy acids) have been widely used in biomedical applications approved by the FDA. Poly (lactide-co-glycolide) acid (PLGA) based on PLA and PGA has been used for fabricating temporary prostheses, 2-5 threedimensional porous scaffolds and films, [6][7][8][9][10][11][12][13][14][15][16][17] controlled/sustained release drug delivery vehicles, [18][19][20][21][22][23][24][25][26] wound closure (surgical sutures and staples), [27][28][29] and implantable thera-peutic devices (orthopedic fixation devices and cardiovascular stents and grafts), [30][31][32][33][34][35][36] all of which have made many significant achievements in tissue engineering, regenerative medicine, gene therapy, controlled drug delivery, and bionanotechnology. 37 PLGA has its hydrolytically labile chemical bonds in its backbone and has been shown to primarily undergo bulk degradation in vivo via chemical hydrolysis of the hydrolytically unstable ester bonds into lactic acid and glycolic acid.…”

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confidence: 99%

The effect of fluid shear stress on the in vitro degradation of poly(lactide‐co‐glycolide) acid membranes

Chu

Zheng

Yao

et al. 2016

J Biomedical Materials Res

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Poly(lactide-co-glycolide) acid (PLGA) has been widely used as a biodegradable polymer material for coating stents or fabricating biodegradable stents. Its mechanism of degradation has been extensively investigated, especially with regard to how tensile and compressive loadings may affect the in vitro degradation of PLGA. Fluid shear stress is also one of the most important factors in the development of atherosclerosis and restenosis. But the effect of fluid shear stress on the degradation process is still unclear. The purpose of this study was to characterize the in vitro degradation of PLGA membranes that experienced different fluid shear stresses in 150 mL of deionized water at 37°C for 20 days. Particular emphasis was given to changes in the viscosity of the degradation solution, as well as the mechanical and morphological properties of the samples. The viscosity of the degradation solution with the mechanical loaded specimens was more severely affected than that of the control group. Increasing the fluid shear stress could accelerate the loss of the ultimate strength of PLGA membranes while it slowed down the change of the tensile elastic modulus in the early period. With regard to morphology, the surface roughness was more obviously reduced in the loaded groups. This indicated that the fluid shear stress could affect the in vitro degradation of PLGA membranes. Therefore, this study could help improve the design of PLGA membranes for biomedical applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2016.

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“…This process leads to generate poly(lactic acid) (PLA) and poly(glycolic acid) (PGA), and the final degradation products are CO 2 and H 2 O which can be excreted from the body by the tricarboxylic acid cycle safely. 2 Regard to its great biocompatibility and good processability, PLGA was approved by the FDA and widely used in biomedical applications such as degradable and absorbable sutures, 3,4 implants, [5][6][7][8][9][10][11] artificial skin graft and scaffolds, [12][13][14][15][16][17][18][19][20][21] and drug release systems. [22][23][24][25][26][27][28][29][30] Especially, as cardiovascular incidents are dramatically increasing, the applications of PLGA in heart patches 31 and polymeric biodegradable stents (BDSs) [32][33][34][35] have been drawn more and more attention.…”

Section: Introductionmentioning

confidence: 99%

Effects of different fluid shear stress patterns on the in vitro degradation of poly(lactide‐co‐glycolide) acid membranes

Chu

et al. 2016

J Biomedical Materials Res

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The applications of poly (lactide-co-glycolide) acid (PLGA) for coating or fabricating polymeric biodegradable stents (BDSs) have drawn more attention. The fluid shear stress has been proved to affect the in vitro degradation process of PLGA membranes. During the maintenance, BDSs could be suffered different patterns of fluid shear stress, but the effect of these different patterns on the whole degradation process is unclear. In this study, in vitro degradation of PLGA membranes was examined with steady, sinusoid, and squarewave fluid shear stress patterns in 150 mL deionized water at 37°C for 20 days, emphasizing on the changes in the viscosity of the degradation solution, mechanical, and morphological properties of the samples. The unsteady fluid shear stress with the same average magnitude as the steady one accelerate the in vitro degradation process of PLGA membranes in terms of maximum fluid shear stress and "window" of effectiveness. Maximum fluid shear stress accelerates the in vitro degradation of molecular fragments that diffused out in the solution while the "window" of effectiveness affects too in the early stage. Besides, maximum fluid shear stress and "window" of effectiveness accelerates the in vitro loss of tensile modulus and ultimate strength of the PLGA membranes while the maximum fluid shear stress plays the leading role in the decrease of tensile modulus at the early degradation stage. This study could help advance the degradation design of PLGA membranes under different fluid shear stress patterns for biomedical applications like stents and drug release systems. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 23-30, 2017.

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Proliferation and differentiation into endothelial cells of human bone marrow mesenchymal stem cells (MSCs) on poly DL-lactic-co-glycolic acid (PLGA) films

Cited by 13 publications

References 8 publications

Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture

Biological character of human adipose-derived adult stem cells and influence of donor age on cell replication in culture

The effect of fluid shear stress on the in vitro degradation of poly(lactide‐co‐glycolide) acid membranes

Effects of different fluid shear stress patterns on the in vitro degradation of poly(lactide‐co‐glycolide) acid membranes

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