Proliferation, migration, and expression of oral‐mucosal‐healing‐related genes by oral fibroblasts receiving low‐level laser therapy after inflammatory cytokines challenge

Basso, Fernanda Gonçalves; Soares, Diana Gabriela; Pansani, Taísa Nogueira; Cardoso, Laís Medeiros; Scheffel, Débora Lopes Salles; Costa, Carlos Alberto de Souza; Hebling, Josimeri

doi:10.1002/lsm.22553

Cited by 65 publications

(33 citation statements)

References 30 publications

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“…The selection of the pore size was based on previous literature studies which used this type of transwell system to evaluate fibroblast migration [14, 15]. It was also considered the characteristics of the fibroblast cell type, such as their size (10–15 um).…”

Section: Methodsmentioning

confidence: 99%

Metallic nanoparticles reduce the migration of human fibroblasts in vitro

et al. 2017

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Nanoparticles have extremely wide applications in the medical and biological fields. They are being used in biosensors, local drug delivery, diagnostics, and medical therapy. However, the potential effects of nanoparticles on target cell and tissue function, apart from cytotoxicity, are not completely understood. Thus, the aim of this study was to investigate the in vitro effects of silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) on human fibroblasts with respect to their interaction with the extracellular matrix and in cell migration. Immunofluorescence analysis revealed that treatment with AgNPs or AuNPs decreased collagen and laminin production at all the concentrations tested (0.1, 1, and 10 μg/mL). Furthermore, cytofluorometric analysis showed that treatment with AgNPs reduced the percentage of cells expressing the collagen receptor very late antigen 2, α2β1 integrin (VLA-2) and the laminin receptor very late antigen 6, α6β1 integrin (VLA-6). In contrast, AuNP treatment increased and decreased the percentages of VLA-2-positive and VLA-6-positive cells, respectively, as compared to the findings for the controls. Analysis of cytoskeletal reorganization showed that treatment with both types of nanoparticles increased the formation of stress fibres and number of cell protrusions and impaired cell polarity. Fibroblasts exposed to different concentrations of AuNPs and AgNPs showed reduced migration through transwell chambers in the functional chemotaxis assay. These results demonstrated that metal nanoparticles may influence fibroblast function by negatively modulating the deposition of extracellular matrix molecules (ECM) and altering the expression of ECM receptors, cytoskeletal reorganization, and cell migration.Electronic supplementary materialThe online version of this article (doi:10.1186/s11671-017-1982-3) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Metallic nanoparticles reduce the migration of human fibroblasts in vitro

et al. 2017

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“…It has been demonstrated by in vitro studies that phototherapy (PT) using laser light modulates cellular functions, such as cell migration and proliferation, even when these cells were exposed to inflammatory cytokines , indicating that this therapy may have biostimulatory effects from the activation of different cell pathways (MAPK/ERK) that induces cellular proliferation . A recent clinical trial also demonstrated the downregulation of several inflammatory mediators as CXCL8, IL‐8 and nitric oxide in saliva after PBM on patients scheduled for hematopoietic stem cell transplantation and planned treatment consisting of a high‐dose chemotherapy myeloablative conditioning regimen compared to control group .…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation in the Metabolism of Lipopolysaccharides‐exposed Epithelial Cells and Gingival Fibroblasts

Pansani

Basso

Soares

et al. 2018

Photochem & Photobiology

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This study assessed the effects of photobiomodulation (PBM) to cells previously exposed to lipopolysaccharides (LPS). Human gingival fibroblasts (HGF) and epithelial cells (HaCaT) were seeded in wells of 24-well plates containing complete culture medium (DMEM). After 24 h, the DMEM was replaced by serum-free DMEM, and cells were exposed to LPS of Escherichia coli (E. coli) (10 μg mL ) for 24, 48, and 72 h. The cells were subjected to specific parameters of phototherapy (PT) (LASERTable-InGaAsP-780 ± 3 nm, 25 mW, 3 J cm ). Cell proliferation (alamarBlue ), viability (Trypan Blue) and synthesis of CCL2 (ELISA) were evaluated. Data were statistically analyzed by the Kruskal-Wallis and Mann-Whitney test (α = 5%). Proliferation and viability of both cell lines decreased after LPS treatment at 48 and 72 h. Enhanced synthesis of CCL2 by gingival fibroblasts occurred at 24 h, while epithelial cells increased synthesis of this chemokine at 48 and 72 h. PBM enhanced cell proliferation and viability in a time-dependent manner for both cell lines exposed or not to LPS, while synthesis of CCL2 by cells exposed to PT decreased over time. PBM caused biomodulatory effects on gingival fibroblasts and epithelial cells previously treated with LPS. These effects may decrease tissue inflammatory response and accelerate wound healing of oral mucosal tissue.

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“…Recently, Góralczyk et al [17] have demonstrated that endothelial cells irradiated at 830 nm secrete less inflammatory mediators, such as TNF-α, and present enhanced cell proliferation when cultured under hyperglycemia conditions. Confirming these results, Basso et al [18] have also shown that PBMT can counteract the negative effects of high concentrations of inflammatory cytokines (TNF-a, IL-1β, IL-6, and IL-8) in gingival fibroblast cell cultures by stimulating their migration and proliferation. These PBMT effects are directly related to the wound-healing process and might contribute to accelerate wound closure processes [18].…”

Section: Pbmt Effect On Different Types Of Cellsmentioning

confidence: 63%

“…Confirming these results, Basso et al [18] have also shown that PBMT can counteract the negative effects of high concentrations of inflammatory cytokines (TNF-a, IL-1β, IL-6, and IL-8) in gingival fibroblast cell cultures by stimulating their migration and proliferation. These PBMT effects are directly related to the wound-healing process and might contribute to accelerate wound closure processes [18].…”

Section: Pbmt Effect On Different Types Of Cellsmentioning

confidence: 63%

Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature

et al. 2017

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This review aims to present and discuss the published data on the application of photobiomodulation therapy (PBMT) on cultured cells, including dentoalveolar-derived mesenchymal stem cells (ddMSCs). The knowledge of the effects of PBMT on ddMSCs is of importance for future application in tissue engineering approaches aiming to regenerate missing dental tissues, among others. The tissue engineering, which is based on the triad stem cells, growth factors, and scaffolds, presents shortcomings that could be circumvented by the effects of the PBMT. Thus, our research group has proposed the PBMT as a fourth element of tissue engineering. This proposition is based on the published data on the effects of PBMT on different cell types, including ddMSCs. Future perspectives on PBMT and ddMSCs will be presented showing translational studies of tissue regeneration induced by PBMT. The experience of our research group supported by other studies of the literature shows the beneficial effects of PBMT. Moreover, this review will show that PBMT on ddMSCs and tissue regeneration is still a huge field for future investigations not only in vitro but also in vivo.

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Proliferation, migration, and expression of oral‐mucosal‐healing‐related genes by oral fibroblasts receiving low‐level laser therapy after inflammatory cytokines challenge

Abstract: LLLT can counteract the negative effects of high concentrations of inflammatory cytokines, especially IL-6 and IL-8 on gingival fibroblast functions directly related to the wound-healing process. Lasers Surg. Med. 48:1006-1014, 2016. © 2016 Wiley Periodicals, Inc.

Cited by 65 publications

References 30 publications

Metallic nanoparticles reduce the migration of human fibroblasts in vitro

Metallic nanoparticles reduce the migration of human fibroblasts in vitro

Photobiomodulation in the Metabolism of Lipopolysaccharides‐exposed Epithelial Cells and Gingival Fibroblasts

Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature

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