Proliferative activity, angiogenesis and nuclear morphometry in renal cell carcinoma

Abstract

Background: Prognostic parameters other than tumor stage and grade are essential for renal cell carcinoma (RCC) patients. This study was undertaken to determine the usefulness of cellular proliferation, angiogenesis and nuclear morphometry in predicting the biological aggressiveness of RCC.Methods: Surgical specimens of 70 patients with RCC were investigated by conventional histology, Ki-67 immunostaining and stereological assessment of angiogenesis and mean nuclear volume.Results: There was no difference in disease-specific survival with respect to sex, age and histopathological type (except sarcomatoid and other types). The survival was significantly lower and the chance of metastases was higher in the group with higher proliferative activity (P = 0.007). There was no relation between angiogenesis, mean nuclear volume, stage and survival. There was a significant relation between both Fuhrman and WHO grades, tumor stage and survival. Histopathological type, grade, angiogenesis and mean nuclear volume failed to predict recurrences and/or metastases. In multivariate analysis, only TNM stage and proliferative activity were found to be independent prognostic factors. Conclusions:In addition to tumor grade and stage, proliferative activity of a given RCC may have the potential to identify patients with an impaired prognosis.

In vivo assessment of the antiproliferative properties of interferon-alpha during immunotherapy: Ki-67 (MIB-1) in patients with metastatic renal cell carcinoma

“…In nephrectomy specimens of nonmetastatic RCC (Tannapfel et al, 1996;Aaltomaa et al, 1997;Kirkali et al, 2001;Rioux-Leclercq et al, 2001) and mRCC (Rioux-Leclercq et al, 2001), Ki-67 (MIB-1) labelling index (LI) has been demonstrated as an independent parameter of unfavourable prognosis.…”

Prognostic Factors in Renal Cell Carcinoma: An Evaluation of T-Stage, Histopathological Grade, p53, Ki-67, COX-2, and Her-2 Expressions

“…It is known that in addition to melanoma, RCC belongs to tumors with a low incidence of p53 mutations compared to, e.g. prostate and bladder cancer (Haitel et al 2000, Kirkali et al 2001, Rioux-Leclercq et al 2000. The low p53 mutation in different cancers (Olivier et al 2002) and the low immunohistochemical staining of RCC tissue blocks for the p53 protein in studies (Haitel et al 2000, Rioux-Leclercq et al 2000 suggest that mutations in p53 result in an accumulation of the p53 protein.…”

“…The incidence of p53-and Ki-67-positive expression in RCC tumors was low in RCC studies (Kankuri-Tammilehto et al 2010, Kirkali et al 2001, Haitel et al 2000, Rioux-Leclercq et al 2000. It is known that in addition to melanoma, RCC belongs to tumors with a low incidence of p53 mutations compared to, e.g.…”

Presence of Tumour-infiltrating FOXP3+Lymphocytes Correlates with Immature Tumour Angiogenesis in Renal Cell Carcinomas

“…For most of solid tumours, microvessel density (MVD) correlates with poor prognosis. However, MVD remains a controversial prognostic predictor for RCC (Kirkali et al, 2001;Sabo et al, 2001a;Suzuki et al, 2001;Dekel et al, 2002;Kinouchi et al, 2003;Yildiz et al, 2008;Qian et al, 2009), a significant contributory factor being that blood vessels in RCC may be regarded as being monolithic in structure and function, which might not accurately reflect the functional diversity based on microvessel differentiation and location. Mature blood vessels are usually covered by pericytes, and pericyte coverage is regarded as an indicator of vascular maturation (Gerhardt and Semb, 2008).…”