Proliferative activity is the most significant predictor of recurrence in noninvasive papillary urothelial neoplasms of low malignant potential and grade 1 papillary carcinomas of the bladder

Pich, Achille; Chiusa, Luigi; Formiconi, Andrea; Galliano, Diego; Bortolin, Paola; Comino, Alberto; Navone, Roberto

doi:10.1002/cncr.10733

Cited by 61 publications

(40 citation statements)

References 44 publications

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“…In cases on non-invasive HG UC and LG UC, the Ki-67 positive rate (index) helps to discriminate between their high grade versus low grade malignant potential based on the estimated number of dysplastic tumour cells. Before the acceptance of the WHO/ISUP classifi cation, some authors described the Ki-67 stain as helpful in recognizing the difference between G1 and G2 papillary carcinomas (13) and pointed out that the Ki-67 was a better marker than the p53 stain (52). The Ki-67 stain represents a useful diagnostic tool, especially in cases, when small tumour fragments only are at disposal.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical characterization of urothelial carcinoma

Rajčáni¹,

Kajo²,

Adamkov³

et al. 2013

BLL

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From the archive of BB Biocyt company, 32 urinary bladder carcinomas (urothelium carcinomas, UC) and 7 cases of chronic cystitis were selected and examined in semiserial sections for the following antigens: 1) cell proliferation marker Ki-67 (expressed in the nuclei), 2) cell cycle regulator p16/INK4a polypeptide (expressed in the cytoplasm and nuclei), 3) urothelium marker p63 (expressed in the nuclei), 4) cytokeratin 7 (CK7). 5) cytokeratin 20 (CK20) and 6) high molecular weight cytokeratin (HMWCK). Invasive urothelium carcinomas showing a high grade dysplasia (invasive HG UC) comprised over the half (20 out of 32) of the investigated tumours. Microinvasion to lamina propria (seen in three HG papillary carcinomas) was regarded as an early infi ltration even when the position of muscular layer could not be determined. Classical invasion across the urinary bladder wall and/or to surrounding tissues was found in 17 cases of low-differentiated HG UCs. The rest (9 out of 32 neoplasms) were either non-invasive papillary carcinomas of high (non-invasive HG UC, 5 cases) or low malignant potential (noninvasive LG UC, 4 cases). Finally, 3 cases were papillary urothelium neoplasms of low malignant potential (PUNLMP). HMWCK was present in all invasive tumours, whereas the frequency of other urothelium markers ranged from 65 to 88 %. Nevertheless, at least two markers were expressed in each invasive tumour. Staining for Ki-67 antigen was positive in over 50 % of the nuclei of HG UCs, while in the LG UCs, the frequency of positive Ki-67 staining did not exceed 25 %. In PUNLMP, the positive rate of Ki-67 stained dysplastic cells was below 10 %. The staining for p16 antigen did not correlate with the degree of dysplasia within urothelium tumours. For routine diagnostic, we recommend to combine the Ki-67 staining with detection of HMWCK. In cases of chronic cystitis, which developed urothelial hyperplasia and/or squamous metaplasia, the presence of p63 antigen was a relevant marker confi rming the urothelial origin of the altered transitional cells (Tab. 6, Fig. 4, Ref. 69)

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical characterization of urothelial carcinoma

Rajčáni¹,

Kajo²,

Adamkov³

et al. 2013

BLL

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“…Su valor como factor predictivo en el cáncer vesical está todavía también por determinar. Los estudios recientes mayoritariamente consideran que la expresión inmunohistoquímica de esta proteína no presenta un valor significativo ni en el análisis univariante ni en el multivariante 1, [30][31][32][33][34] . En tumores pT1G3, Wolf HK y cols.…”

Section: Inmunohistoquímica De Bcl-2unclassified

Valor pronóstico de progresión de las moléculas reguladoras del ciclo celular en tumores vesicales T1G3

et al. 2005

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RESUMEN VALOR PRONÓSTICO DE PROGRESIÓN DE LAS MOLÉCULAS REGULADORAS DEL CICLOCELULAR EN TUMORES VESICALES T1G3 Introducción y Objetivos: El tumor vesical T1G3 constituye el grupo de tumores superficiales más agresivo. Nuevos factores pronósticos en el campo de la citogenética y la biología molecular han sido analizados, con resultados a menudo contradictorios, siendo escasos los trabajos específicos en tumores T1G3. Nuestro objetivo es determinar si en este grupo de tumores los marcadores inmunohistoquímicos presentan valor predictivo de progresión clínicamente útil, y por tanto con validez para indicar una actitud terapéutica precoz más idónea.Material y Métodos: Estudio retrospectivo de una serie de 83 pacientes afectos de tumor vesical T1G3, sobre los que analizamos un total de 14 variables; entre los nuevos factores predictivos: la determinación inmunohistoquímica de las proteínas reguladoras del ciclo celular: p53, p21 y bcl-2, así como la proteína Ki-67 como marcador de proliferación celular. Mediante análisis de regresión logística establecemos las variables pronósticas independientes para progresión tumoral.Resultados: El punto de corte establecido para Ki67 y p53 fue el 40% de células inmunomarcadas, 20% para p21 y 10% para Bcl-2. El análisis univariante puso de manifiesto diferentes tasas de progresión y tiempos libres de progresión en función de la inmunotinción de Ki67 y p53; sin embargo, la regresión logística demostró que solo la inmunohistoquímica de p53 presentaba valor predictivo independiente.Conclusiones: La determinación inmunohistoquímica de p53 presenta valor predictivo de progresión clínicamente útil en tumores vesicales T1G3, de manera que su determinación puede constituir un factor fundamental en las estrategias de tratamiento de estos tumores.Palabras clave: T1G3. Inmunohistoquímica. p53. ABSTRACT PROGNOSTIC VALUE FOR PROGRESSION OF THE REGULATING PROTEINS OF THE CELLULAR CYCLE IN PT1G3BLADDER TUMOURS Introduction and objective: Bladder tumor T1G3 constitutes the group of superficial tumors more aggressive. New prognostic factors in the field of the cytogenetics and molecular biology have been analyzed, with often contradictory results, being little the specific works in tumors T1G3. Our objective is to determine if in this group of tumors the immunohistochemical markers present predictive value of clinically useful progression, and therefore with validity to indicate more suitable a precocious therapeutic attitude Material and methods: Retrospective study of a series of 83 patients affected of bladder tumor T1G3, on which we analyzed a total of 14 variables; between the new predictive factors: the immunohistochemical determination of regulating proteins of the cellular cycle: p53, p21 and bcl-2, as well as the Ki-67 protein like marker of cellular proliferation. By means of logistic regression analisys we establish the independent prognostic variables for tumorlike progression.Results: The cut point established for Ki67 and p53 was 40% of inmmunomarked cells, 20% for p21 and 10% for Bc...

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“…Ki-67 is a nuclear antigen expressed in the G1, S, and G2, but not the G0, phases of the cell cycle. Tumor recurrence and progression are significantly associated with the Ki-67 index in non-muscle invasive bladder cancers [14][15][16].…”

Section: Introductionmentioning

confidence: 99%