Proliferative potential in benign mixed salivary gland tumors, using Ki67 marker in relation to different clinicopathological parameters

Abstract

7Histologically a wide variety of structures may be seen. These varied tissues are Background and objective: Pleomorphic adenoma is the most common benign tumor of the salivary gland. It shows a remarkable degree of morphological diversity. Immunohistochemical Ki67 expression is used to predict the proliferative potential of the growth. The aim of this study was to evaluate the expression of the proliferation marker Ki-67 in benign mixed salivary gland tumors. Methods: Fifty five paraffin embedded tissue blocks were included in the study. After histopathological reassessment of hematoxylin and eosin stained section for each block, an immunohistochemical staining was performed using anti-Ki67 protein.Results: From total number of 55 cases, 58.1% of patients were in group aged ≤ 40 years. The male to female ratio was 1.037:1. The parotid gland was the most affected (56.37%) gland. The mean duration of lesions before treatment was 17.96 months and the mean size of tumors was 3.76 cm. Histologically, cell-rich subtype constituted 54.54%, followed by stroma-rich (34.55%), and only 10.91% of cases were classic. Ki67 expression was positive in 18.18% predominantly in epithelial variant with a weak score in nine cases (16.36%). Six of positive cases were in parotid gland and two cases in the submandibular gland. Only one case was in minor salivary gland. Moderate Ki67 expression score was seen in one epithelial variant case (14.28 %) associated with parotid gland. Conclusion: Immunohistochemical expression of Ki-67 in pleomorphic adenoma was characterized by low proliferative rate and predominantly seen in epithelial tumor variants.

CD3 and CD20 Expressions and Infiltrating Patterns in Salivary Gland Tumors

“…The demographic data of the selected sample do not represent the regional population. However, the age incidences of the total sample showed peak values in the third and fifth decades; nonetheless, benign tumors (PAs) with smaller sizes were recorded more frequently among patients who were younger in age in comparison with the records of patients with malignant tumors; a similar result was found in other clinicopathological records [27][28][29]. This result could be related to the early recognition of such tumors as periauricular swelling.…”

“…The available clinicopathological data (sex, age, site, tumor size, and lymph node status) were obtained from patients' reports. The patients were divided into three age groups: (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40), (41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60), (61-80). The diagnosis was confirmed by a newly prepared H&Estained tissue section, and CD117 immunostaining positivity was applied to verify the diagnosis of AdCC.…”

Mitotic Index and Immunohistochemical Expression of Ki-67 in Pleomorphic Adenoma of Salivary Glands

“…The majority of studies reported Ki-67 expression is negative to weak positive and some studies reported moderate to strong positive. 11,12,16,17,18,19,20,21,25 The results of these studies indicate that biological behavior of PAs is different in cases which recur and transform to malignancy and also show high expression of Ki-67. During routine histopathological examination of PAs when mitotic figures are observed more, then Ki-67 expression may be suggested to observe its early transformation to malignancy (CaExPAs).…”

“…Literature revealed role of Ki-67 in Pleomorphic adenoma and its variable expression. 9,10,11,12 The biological behavior of PA is variable in few cases due to its transformation into CaExPA. Its diagnosis is usually made by conventional histopathological examination and potential malignant transformation is determined by counting mitotic activity however role of immunohistochemistry i.e.…”