Proline Accumulates in Response to Higher Temperatures during Dehardening in Peach Shoot Tissues

Shin, Hyunsuk; Oh, Sewon; Kim, Keumsun; Kim, Daeil

doi:10.2503/hortj.mi-088

Cited by 9 publications

(8 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A significant increase in Pro content when cold hardened, eco-dormant shoots were exposed to HT1 (Fig. 2) further confirms our previous results (Shin et al 2016). It is noteworthy that Pro content remained unchanged when HT1 plants were transferred to CT but it further increased when CT plants were exposed to warm temperatures, i.e., HT2.…”

Section: Gene Abbreviationsupporting

confidence: 88%

“…Interestingly, during warm-dehardening, the expression of P5CS decreased while that of P5CR and OAT increased. Based on our results we had hypothesized that GSA and P5C production and Pro bio-synthesis during dehardening occurred via Orn pathway in peach shoot tissues (Shin et al 2016).…”

Section: Introductionmentioning

confidence: 74%

“…Moreover, not much is known about the fate of Pro during warminduced dehardening, especially in woody perennials. We have previously observed that Pro content of shoots from 11 peach cultivars, including 'Daewol' peach, increased when naturally cold-hardened plants were dehardened by an exposure to warmer temperatures (Shin et al 2016). This finding prompted the question as to whether this enhancement is a response to exposing cold acclimated plants to sudden warm temperatures (i.e., high temperature stress) or related to plant's preparation for growth resumption in eco-dormant shoots.…”

Section: Discussionmentioning

confidence: 99%

“…Total RNA extraction and quantitative PCR were performed as described in Shin et al (2016). Relative gene expression was calculated using gene expression for BT samples for study 1 and 2 taken as 1.…”

Section: Rna Extraction and Relative Gene Expression Analysismentioning

confidence: 99%

“…In a previous study, we have shown that Pro accumulated in the shoots of peach (Prunus persica) cultivars when naturally (out-door) cold-hardened plants were dehardened either in the field (spring-de-acclimation) or artificially [controlled exposure to 23.2/18.5°C (D/N)] (Shin et al 2016). Interestingly, during warm-dehardening, the expression of P5CS decreased while that of P5CR and OAT increased.…”

Section: Introductionmentioning

confidence: 96%

See 4 more Smart Citations

Proline accumulation in response to high temperature in winter-acclimated shoots ofPrunus persica: a response associated with growth resumption or heat stress?

Shin¹,

Oh²,

Arora³

et al. 2016

Can. J. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Our previous study revealed that proline (Pro) accumulated in peach trees when cold-acclimated shoots were exposed to higher temperatures. In this study, we determined whether such Pro accumulation is indeed a high temperature-stress response or metabolic preparation for growth resumption. Two studies were conducted to address this question using 'Daewol' peach trees. In study 1 (February), Pro content of eco-dormant shoots increased when cold-acclimated plants were exposed to two heat treatments (HTs) but remained unchanged during the intervening cold treatment (CT). Concurrently, ornithine-delta-aminotransferase (OAT) and Δ 1-pyrroline-5carboxylate reductase (P5CR) genes were up-and down-regulated after two HTs and the CT, respectively. Study 2, conducted with growing trees (May), showed that Pro did not change and expression of P5CR and OAT was downregulated when trees were subjected to either HT or CT; HT and CT used here constituted 'stress' treatments as revealed by the decline in chlorophyll fluorescence. Taken together, these results suggest that Pro accumulation (study 1) may be associated with the preparation for growth resumption and not 'stress'. Furthermore, results from parallel gene expression studies suggest that the ornithine, rather than glutamate, pathway may be the primary Pro biosynthesis route both during growth resumption and high temperature stress in 'Daewol' peach.

show abstract

Section: Gene Abbreviationsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Rna Extraction and Relative Gene Expression Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 3 more Smart Citations