Proline and functioning of the antioxidant system in Thellungiella salsuginea plants and cultured cells subjected to oxidative stress

Soshinkova, T. N.; Radyukina, N. L.; Korolkova, D. V.; Носов, А. В.

doi:10.1134/s1021443713010093

Cited by 53 publications

(27 citation statements)

References 21 publications

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“…Proline is accumulated preferentially in leaves in order to maintain chlorophyll level and cell turgor to protect photosynthetic activity under salt stress (Silva-Ortega et al 2008). Proline has also a potential role in scavenging ROS products (Soshinkova et al 2013). The accumulation of proline in plants under stress is caused either by the induction of expression of proline biosynthesis genes ( P5CS, P5CR ) or by the repression of the genes of its degradation pathway (PDH silencing) (Marco et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Effect of salt stress on ion concentration, proline content, antioxidant enzyme activities and gene expression in tomato cultivars

Gharsallah¹,

Fakhfakh²,

Grubb³

et al. 2015

AoB PLANTS

209

113

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Understanding plant response to salinity, one of the major abiotic stresses, provides insights into the improvement of tomato salt tolerance. This work focuses on the responses of tomato cultivars to salt stress. Genotypes, representative of content and enzyme activities. QPCR analysis of WRKY, ERF, LeNHX and HKT genes was also performed. A high K+, Ca2+ and proline accumulation as well as a decrease in Na+ concentration mediated salt tolerance. Concomitant with a pattern of high antioxidant enzyme activities, tolerant genotypes also displayed differential patterns of gene expression.

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Section: Discussionmentioning

confidence: 99%

Effect of salt stress on ion concentration, proline content, antioxidant enzyme activities and gene expression in tomato cultivars

Gharsallah¹,

Fakhfakh²,

Grubb³

et al. 2015

AoB PLANTS

209

113

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“…Plant organelle contain important enzymes at high concentration which split H 2 O 2 into H 2 O and O 2 (Henzler and Steudle ). Accumulated H 2 O 2 in the cell was further reduced by the activity of catalase and ascorbate peroxidase system (Willekens et al , Soshinkova et al ). Plants synthesize NO in different enzymatic and non‐enzymatic pathways (Wodala et al ).…”

Section: Discussionmentioning

confidence: 99%

“…3B,C) because higher amount of acetate group of CM-H 2 DCFDA was cleaved by esterases and its thiol-reactive chloromethyl group reacts with glutathione and other thiols (Bradley et al 2014 (Henzler and Steudle 2000). Accumulated H 2 O 2 in the cell was further reduced by the activity of catalase and ascorbate peroxidase system (Willekens et al 1997, Soshinkova et al 2013. Plants synthesize NO in different enzymatic and non-enzymatic pathways (Wodala et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Sterol glycosyltransferases required for adaptation of Withania somnifera at high temperature

Singh

Tiwari

Singh

et al. 2017

Physiologia Plantarum

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Heat is a major environmental stress factor that confines growth, productivity, and metabolism of plants. Plants respond to such unfavorable conditions through changes in their physiological, biochemical and developmental processes. Withania somnifera, an important medicinal plant, grows in hot and dry conditions, however, molecular mechanisms related to such adaptive properties are not known. Here, we elucidated that members of the sterol glycosyltransferases (SGT) gene family play important roles in the survival of W. somnifera under adverse conditions through maintaining the integrity of the membrane. SGTs are enzymes involved in sterol modifications and participate in metabolic flexibility during stress. Silencing of WsSGT members, for instance WsSGTL1, WsSGTL2 and WsSGTL4, was inimical for important physiological parameters, such as electron transport rate, photochemical quantum yield, acceptor side limitation, non-photochemical quenching (NPQ), Fv/Fm and net photosynthetic rate, whereas stomatal conductance, transpiration rate and dark respiration rates (Rds) were increased. Decreased NPQ and increased Rds helped to generate significant amount of ROS in the Wsamisgt lines. After heat stress, H O , lipid peroxidation and nitric oxide production increased in the Wsamisgt lines due to high ROS generation. The expression of HSPs in Wsamisgt lines might be involved in regulation of physiological processes during stress. We have also observed increased proline accumulation which might be involved in restricting water loss in the Wsamisgt lines. Taken together, our observations revealed that SGTL enzyme activity is required to maintain the internal damages of the cell against high temperature by maintaining the sterol vs sterol glycosides ratio in the membranes of W. somnifera.

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“…Also, in our study, enhanced antioxidant defense enzymes in spermidine and proline treated peppers decreased H 2 O 2 and MDA content. Proline and spermidine are reactive oxygen scavengers, as Hossain and Fujita (2010) and Soshinkova et al (2013) reported, with these protective properties against oxidative damage, the lipid peroxidation levels decrease.…”

Section: Discussionmentioning

confidence: 99%

“…The studies conducted demonstrated that spermidine (spd) is participating in the regulation of plant responses to diverse abiotic stresses like drought or osmotic stress, salinity, heat and chilling through direct binding to membrane phospholipids, osmotic adjustment, scavenging of free radicals and maintaining a cation-anion balance (Kusano et al, 2008). In vitro studies have shown that proline is a reactive oxygen scavenger and therefore its protective property may have an antioxidant effect (Soshinkova et al, 2013). For this reason, the exogenous polyamine and proline applications are an effective approach which may increase the tolerance to the stress and the product yield.…”

Section: Introductionmentioning

confidence: 99%

Pretreatment with spermidine and proline reverses inhibitory effects of Phytophthora capsici stress in pepper

Koç

Arıcı

İşlek

2016

Zemdirbyste-Agriculture

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Phytophthora blight (Phytophthora capsici Leon.), which is one of the most important disease factors incurring great economic losses, causes root crown rot. When pepper seedlings were pretreated with 0.1 mM spermidine (spd), 1 mM spd, 0.1 mM spd + 1 mM proline, 0.1 mM spd + 10 mM proline, 1 mM spd + 1 mM proline and 1 mM spd + 10 mM proline and then exposed to P. capsici, they improved their tolerance to subsequent P. capsici-induced oxidative damage. It was detected that cultivar 'Sirena RZ F1' was resistant to P. capsici and it was determined that cultivar 'Sera Demre-8' exhibited sensitive reaction. P. capsici stress caused an increase in total phenolic, malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) levels, as well as peroxidase (POX) and polyphenol oxidase (PPO) activities in leaves of pepper seedlings. Application of exogenous spermidine and spermidine + proline further increased PPO and POX activity, total phenolic amount. On the other hand, these applications in two cultivars and all days decreased the disease severity, necrosis length and MDA and H 2 O 2 amounts. These data show that antioxidants, especially polyphenol oxidase, peroxidase and total phenolic appear to protect pepper seedlings against stress-related damage. Moreover, spermidine and spermidine + proline have the potential to scavenge directly free radicals and promote the activity of antioxidant enzymes in pepper seedlings under P. capsici stress. In addition, it was also identified that especially 0.1 mM spd, 1 mM spd and 0.1 mM spd + 1 mM proline applications decreased the colony growth of P. capsici and the amount of zoospores. Therefore, we suggest that spermidine and spermidine + proline could play a protective role in protecting the pepper seedlings from P. capsici stress.

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Proline and functioning of the antioxidant system in Thellungiella salsuginea plants and cultured cells subjected to oxidative stress

Cited by 53 publications

References 21 publications

Effect of salt stress on ion concentration, proline content, antioxidant enzyme activities and gene expression in tomato cultivars

Effect of salt stress on ion concentration, proline content, antioxidant enzyme activities and gene expression in tomato cultivars

Sterol glycosyltransferases required for adaptation of Withania somnifera at high temperature

Pretreatment with spermidine and proline reverses inhibitory effects of Phytophthora capsici stress in pepper

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