Proline synthesis and catabolism-related genes synergistically regulate proline accumulation in response to abiotic stresses in grapevines

Wei, Tong-Lu; Wang, Ze-Xian; He, Yu-Fan; Xue, Shuo; Zhang, Shuai-Qi; Liu, Hainan; Yu, Yi‐He; Guo, Da‐Long

doi:10.1016/j.scienta.2022.111373

Cited by 8 publications

(9 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pro activates floral shoot apical meristems for flower development and enhances the activity of various enzymes ( Deuschle et al., 2004 ; Kavi Kishor and Sreenivasulu, 2014 ; Kavi Kishor et al., 2015 ). Pro also controls the dynamics of numerous genes and significantly influences overall plant growth and development ( Szabados and Savouré, 2010 ; Hildebrandt et al., 2015 ; Shin et al., 2018 ; Wei et al., 2022 ). Pro is synthesized from Glu (cytosol and plastids) or ornithine (Orn) (mitochondria) via a two-step enzymatic reaction that shares the intermediate P5C ( Supplementary Figure 4 ) ( Szabados and Savouré, 2010 ; Verslues and Sharma, 2010 ; Wang et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Generally, P5CS, OAT, and PDH control the transcriptional regulation, while P5CR and P5CDH are involved in both transcriptional and post-transcriptional processes ( Giberti et al., 2014 ). In addition, in Pro metabolism, P5CS , P5CR , and OAT genes are related to biosynthesis, whereas P5CDH and PDH genes are associated with catabolism ( Wei et al., 2022 ). Pro biosynthesis is activated, and its catabolism is repressed under stress conditions.…”

Section: Discussionmentioning

confidence: 99%

“…When ideal conditions are reestablished, the opposite regulation is triggered ( Xue et al., 2009 ). Furthermore, the accumulation of Pro may be related to the degree of tolerance, and the positive correlation of Pro accumulation depends on the species and their adaptation to stress and normal physiological conditions ( Hien et al., 2003 ; Mattioli et al., 2009 ; Xue et al., 2009 ; Wei et al., 2022 ). However, to the best of our knowledge, there is still a lack of understanding of Pro metabolism, particularly in relation to flowering development and delay mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, high expression levels of the P5CS , P5CR , and PDH genes were observed during the development of meristematic tissues, including the shoot apex, root tips, and inflorescences, as well as during stress responses ( Rizzi et al., 2015 ). In particular, Wei et al. (2022) reported that P5CS2 , OAT , and PDH are highly expressed in grapevine flowers.…”

Section: Discussionmentioning

confidence: 99%

“…Metabolic modifications of Pro, such as osmolytes, ROS scavengers, and protein chaperones, can reduce cell damage ( Rizzi et al., 2015 ). Pro is also involved in various developmental processes such as growth, cell division, differentiation, flowering, and embryo development ( Szabados and Savouré, 2010 ; Kavi Kishor et al., 2015 ; Shin et al., 2016 ; Wei et al., 2022 ). In particular, Pro accumulation has been observed in tomato plants exposed to warm temperatures during flowering ( Schwacke et al., 1999 ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Physiological and metabolic analyses reveal the proline-mediated flowering delay mechanism in Prunus persica

Park,

Muthuramalingam,

Jeong

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Peaches are susceptible to various environmental stresses. Particularly in late spring, freezing temperatures can damage peaches and consequently, affect their productivity. Therefore, flowering delay is a prominent strategy for avoiding spring frost damage. Our previous study confirmed that treatment with 5% sodium alginate and 100 mM CaCl2 (5AG) to avoid frost damage during the blooming stage delays flowering. To reveal the flowering delay mechanism of peaches, this study systematically analyzed the modification of amino acid profiles in control and 5AG-treated peach plants at different day intervals. Our findings indicate that arginine (Arg), glutamate (Glu), and proline (Pro) levels differed between the control and 5AG-treated peach shoots throughout the phenological development of flower buds. Furthermore, two amino acids (Arg and Glu) are involved in the Pro pathway. Thus, using a computational metabolomics method, Pro biosynthesis and its characteristics, gene ontology, gene synteny, cis-regulatory elements, and gene organizations were examined to decipher the involvement of Pro metabolism in peach flowering delay. In addition, qRT-PCR analysis revealed the transcriptional regulation of Pro-related and flowering-responsive genes and their role in flowering delay. Overall, this pilot study provides new insights into the role of Pro in the flowering delay mechanisms in Prunus persica through 5AG treatment.

show abstract