Prolonged Endochondral Bone Healing in Senescence is Shortened by Low-Intensity Pulsed Ultrasound in a Manner Dependent on COX-2

Naruse, Keiko; Sekiya, Hiroo; Harada, Yoshihumi; Iwabuchi, Sadahiro; Kozai, Yusuke; Kawamata, Ryota; Kashima, Isamu; Uchida, Kentaro; Urabe, Ken; Seto, Kannichi; Itoman, Moritoshi; Mikuni-Takagaki, Yuko

doi:10.1016/j.ultrasmedbio.2010.04.011

Cited by 41 publications

(30 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Possible signal molecules, which involved in the promoting effects of LIPUS in the present study, might be the followings: Prostaglandin E2, nitric oxide, vascular endothelial growth factors (VEGF) because enhancement of the production of these signal molecules by mechanical stress has been reported [22][23][24]. Although the precise mechanism of the present enhancement of bone regeneration is not clear, it is likely that LIPUS stimulated cellular migration, proliferation and differentiation which were controlled by these signal molecules [25,26]. It has been reported that LIPUS stimulated angiogenesis and the most effective time point of LIPUS application was at the second week when extensive angiogenesis generally occurs after the surgery [27,28].…”

Section: Figurementioning

confidence: 67%

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Chen¹,

Jia²,

Noritake³

et al. 2013

OJRM

View full text Add to dashboard Cite

Purpose: Low intensity pulsed ultrasound stimulation (LIPUS) has been clinically applied to promote bone fracture healing in the orthopedic field. Thus, it is likely that LIPUS also stimulates bone regeneration in bone defects in the cranial-maxillofacial area. However, this has not been clearly proved. Furthermore, optimal time point and period of the application after the surgery has not been reported. The purpose of the present study was to evaluate the effect of LIPUS on bone regeneration in the rat parietal bone defects especially focusing on time and period of the application. Materials and Methods: Eighteen Wistar rats (14 weeks old) were divided into 6 groups: 5 experimental groups and a control group. Bone defect of 5 mm diameter was prepared on each side of the parietal bone and customized gelatin membranes were placed over the bone defects. LIPUS (160 mW/cm 2 , 15 min/day) was applied to the defect area with an active transducer externally in the experimental groups according to the schedules of the applications: Group 1 (day 6 -12), group 2 (day 13 -19), group 3 (day 20 -26), group 4 (day 6 -19) and group 5 (day 6 -26). All the animals were sacrificed at 28 days. The defects were analyzed with micro CT and then histologically. Results: In Group 1, new bone formation was significantly promoted and the newly-formed bone was thick and matured compared to the one of the control group. In other experimental groups there were tendencies of stimulation of new bone formation; however, they were not statistically significant. Discussion and Conclusion: The present study demonstrated that amount of new bone formation in the bone defect depended on the time and period of LIPUS application. It has been suggested that application of LIPUS at an early healing period, the second week after the surgery, effectively accelerated new bone formation.

show abstract

Section: Figurementioning

confidence: 67%

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Chen¹,

Jia²,

Noritake³

et al. 2013

OJRM

View full text Add to dashboard Cite

show abstract

“…LIPUS can affect all phases of fracture healing including inflammatory, intramembranous ossification, chondrogenesis, endochondral ossification, and remodeling phase in a positive way. [1819202122]…”

Section: Discussionmentioning

confidence: 99%

Effect of Low-intensity pulsed ultrasound on postorthognathic surgery healing process

Tehranchi

Badiee

Younessian

et al. 2017

Ann Maxillofac Surg

View full text Add to dashboard Cite

“…19 Pulsed ultrasound also has been shown to increase COX-2 expression at mouse fracture sites, suggesting that extracorporeal mechanical stimulation of fracture healing may be mediated by enhanced COX-2 activity. 20 Cyclooxygenase-1 Few studies have specifically examined the effects of lost COX-1 activity on fracture healing. Preliminary experiments in COX-1-null mice showed that these mice developed normal fracture calluses suggesting that COX-1 is not critical for fracture healing.…”

Section: The Role Of Lipid Mediator Synthesis Enzymes In Fracture Heamentioning

confidence: 99%

Fracture healing and lipid mediators

O’Connor¹,

Manigrasso²,

Kim³

et al. 2014

BoneKEy Reports

View full text Add to dashboard Cite

Lipid mediators regulate bone regeneration during fracture healing. Prostaglandins and leukotrienes are well-known lipid mediators that regulate inflammation and are synthesized from the O-6 fatty acid, arachidonic acid. Cyclooxygenase (COX-1 or COX-2) and 5-lipoxygenase (5-LO) catalyze the initial enzymatic steps in the synthesis of prostaglandins and leukotrienes, respectively. Inhibition or genetic ablation of COX-2 activity impairs fracture healing in animal models. Genetic ablation of COX-1 does not affect the fracture callus strength in mice, suggesting that COX-2 activity is primarily responsible for regulating fracture healing. Inhibition of cyclooxygenase activity with nonsteroidal anti-inflammatory drugs (NSAIDs) is performed clinically to reduce heterotopic ossification, although clinical evidence that NSAID treatment impairs fracture healing remains controversial. In contrast, inhibition or genetic ablation of 5-LO activity accelerates fracture healing in animal models. Even though prostaglandins and leukotrienes regulate inflammation, loss of COX-2 or 5-LO activity appears to primarily affect chondrogenesis during fracture healing. Prostaglandin or prostaglandin analog treatment, prostaglandin-specific synthase inhibition and prostaglandin or leukotriene receptor antagonism also affect callus chondrogenesis. Unlike the O-6-derived lipid mediators, lipid mediators derived from O-3 fatty acids, such as resolvin E 1 (RvE1), have anti-inflammatory activity. In vivo, RvE1 can inhibit osteoclastogenesis and limit bone resorption. Although O-6 and O-3 lipid mediators have clear-cut effects on inflammation, the role of these lipid mediators in bone regeneration is more complex, with apparent effects on callus chondrogenesis and bone remodeling.

show abstract

Prolonged Endochondral Bone Healing in Senescence is Shortened by Low-Intensity Pulsed Ultrasound in a Manner Dependent on COX-2

Cited by 41 publications

References 36 publications

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Effects of low intensity pulsed ultrasound stimulation on bone regeneration in rat parietal bone defect model

Effect of Low-intensity pulsed ultrasound on postorthognathic surgery healing process

Fracture healing and lipid mediators

Contact Info

Product

Resources

About