Prolonged proliferation and delayed senescence of the adipose-derived stem cells grown on the electrospun composite nanofiber co-encapsulated with TiO2 nanoparticles and metformin-loaded mesoporous silica nanoparticles

Pourpirali, Raheleh; Mahmoudnezhad, Aydin; Oroojalian, Fatemeh; Zarghami, Nosratollah; Pilehvar, Younes

doi:10.1016/j.ijpharm.2021.120733

Cited by 42 publications

(14 citation statements)

References 51 publications

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“…However, MET@MSNs exhibited a larger size (average diameter: ~ 90.71 nm) and no significant mesopores on the surface. This result was similar with that of previous studies [ 22 , 26 ]. Furthermore, we determined the specific surface areas of the MSN and MET@MSNs using an N2 adsorption analyser, with the pore-size distribution and surface area analysed by Barrett–Joyner–Halenda and Brunauer–Emmett–Teller (BET) procedures, respectively (Fig.…”

Section: Resultssupporting

confidence: 94%

“…After synthesizing porous MET@MSNs nanospheres according to a previously described method [ 26 ], we evaluated the morphological characteristics of MSN and MET@MSNs using scanning electron microscopy (SEM) (Fig. 1 a, b).…”

Section: Resultsmentioning

confidence: 99%

“…MET@MSNs were synthesized as previously described [ 22 , 59 ]. Briefly, MSN (20 mg; XFNANO, Nanjing, China) and Met (60 mg; MACKLIN, Shanghai, China) were dispersed in ethanol and stirred at 37 °C for 24 h. The mixture was centrifuged at 10,000 rpm for 10 min, the supernatant was discarded, and the pellet was resuspended in ethanol and dried at 80 °C for ~ 8 h for further studies.…”

Section: Methodsmentioning

confidence: 99%

“…These advantages have made them applicable in various biomedical applications, including tumour treatment [ 22 ], drug delivery platform [ 23 ], anti-infective [ 24 ], biocatalysts [ 25 ]. The use of MSN-loaded metformin (MET@MSNs) is an effective drug-delivery method for the controlled release and topical application, with previous studies demonstrating that MET@MSNs exhibit a well-controlled release capacity and immunomodulatory properties for the treatment of tumours [ 22 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

et al. 2022

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Background The complicated hyperglycaemic and chronic inflammation of diabetic wounds in orthopaedic surgery leads to dysregulated immune cell function and potential infection risk. Immune interventions in diabetic wounds face a possible contradiction between simultaneous establishment of the pro-inflammatory microenvironment in response to potential bacterial invasion and the anti-inflammatory microenvironment required for tissue repair. To study this contradiction and accelerate diabetic-wound healing, we developed a photocurable methacryloxylated silk fibroin hydrogel (Sil-MA) system, co-encapsulated with metformin-loaded mesoporous silica microspheres (MET@MSNs) and silver nanoparticles (Ag NPs). Results The hydrogel system (M@M–Ag–Sil-MA) enhanced diabetic-wound healing via spatiotemporal immunomodulation. Sil-MA imparts a hydrogel system with rapid in situ Ultra-Violet-photocurable capability and allows preliminary controlled release of Ag NPs, which can inhibit bacterial aggregation and create a stable, sterile microenvironment. The results confirmed the involvement of Met in the immunomodulatory effects following spatiotemporal dual-controlled release via the mesoporous silica and Sil-MA. Hysteresis-released from Met shifts the M1 phenotype of macrophages in regions of diabetic trauma to an anti-inflammatory M2 phenotype. Simultaneously, the M@M–Ag–Sil-MA system inhibited the formation of neutrophil extracellular traps (NETs) and decreased the release of neutrophil elastase, myeloperoxidase, and NETs-induced pro-inflammatory factors. As a result of modulating the immune microenvironmental, the M@M–Ag–Sil-MA system promoted fibroblast migration and endothelial cell angiogenesis in vivo, with verification of enhanced diabetic-wound healing accompanied with the spatiotemporal immunoregulation of macrophages and NETs in a diabetic mouse model. Conclusions Our findings demonstrated that the M@M–Ag–Sil-MA hydrogel system resolved the immune contradiction in diabetic wounds through spatiotemporal immunomodulation of macrophages and NETs, suggesting its potential as a promising engineered nano-dressing for the treatment of diabetic wounds in orthopaedic surgery. Graphical Abstract

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Section: Resultssupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

et al. 2022

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“…The %EE and %LC were measured using the following formulations (Mirhosseini et al, 2020;Pourpirali et al, 2021;Abtahi et al, 2022): EE(%) encapsulated drug mg Total used drugs mg × 100; LC(%) weight of encapsulated drugs weight of LBL − NPs × 100.…”

Section: Drug Content and Encapsulation Efficiencymentioning

confidence: 99%

RETRACTED: Targeted delivery of 5-fluorouracil, miR-532-3p, and si-KRAS to the colorectal tumor using layer-by-layer liposomes

Shahidi

Abazari

Dayati

et al. 2022

Front. Bioeng. Biotechnol.

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Co-delivery of siRNA or miRNA with chemotherapeutic drugs into tumor sites is an attractive synergetic strategy for treating colorectal cancer (CRC) due to their complementary mechanisms. In the current work, a liposome nanoparticle (Huang et al., Cancer Metastasis Rev., 2018, 37, 173–187) coated by cationic chitosan (CS) using a controlled layer-by-layer (LbL) process was designed to deliver simultaneous si-KRAS, miRNA-532-3p, and 5-Fluorouracil (5-FU) into CRC cells. The LbL NPs exhibited a spherical structure with an average size of 165.9 nm and effectively protected si-KRAS and miRNA-532-3p against degradation by serum and nucleases. Interestingly, the LbL NPs were successfully entered into cells and efficiently promoted cytotoxicity and suppressed cancer cell migration and invasion. In vivo, the LbL NPs reduced tumor growth in SW480-tumor-bearing mice models. In conclusion, these results suggested that the LbL NPs co-loaded with 5-FU and miR-532-3p/si-KRAS might provide a promising potential strategy for inhibiting the malignant phenotypes of CRC cells.

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Spatiotemporal On–Off Immunomodulatory Hydrogel Targeting NLRP3 Inflammasome for the Treatment of Biofilm‐Infected Diabetic Wounds

Zhou

Mei

Liu

et al. 2023

Adv Funct Materials

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Biofilm‐infected diabetic wounds (BIDWs) with hyperglycemia and bacterial colonization are characterized by disordered inflammation and abnormal activation of NLRP3 inflammasome, leading to sustained macrophage M1 polarization and neutrophil extracellular traps (NETs) formation. An immoderate anti‐inflammatory treatment that downregulates NLRP3 in turn promotes the persistence of biofilm infections and impairs the healing of BIDWs. Therefore, reconciling biofilm elimination and immune regulation holds the promise of curing BIDWs. Herein, a novel spatiotemporal on–off immunomodulatory therapy (SOIT) is proposed for treating BIDWs through biphasic regulation of NLRP3. Methacrylate gelatin hydrogels (Gel‐MA) incorporated with graphene oxide (GO) and metformin‐loaded mesoporous silicone nanospheres are synthesized and photo cross‐linked to construct a nanocomposite hydrogel (MGO@GM). First, GO nanosheets released from MGO@GM inhibit bacterial biofilm formation and disrupt mature biofilms under near‐infrared irradiation. Meanwhile, GO activates the NLRP3 to induce a macrophage‐associated proinflammatory response against biofilms. Afterward, with the subsequent degradation of MGO@GM, released metformin reduces local hyperglycemia, downregulates NLRP3, and inhibits NETs formation. Furthermore, repolarized M2 macrophages alleviate the inflammatory microenvironment and promote tissue regeneration. Briefly, this SOIT strategy regulates the NLRP3 and rescues impaired innate immunity to facilitate anti‐infection and tissue repair, which provides a new perspective for the future clinical treatment of BIDWs.

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Prolonged proliferation and delayed senescence of the adipose-derived stem cells grown on the electrospun composite nanofiber co-encapsulated with TiO2 nanoparticles and metformin-loaded mesoporous silica nanoparticles

Cited by 42 publications

References 51 publications

An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

RETRACTED: Targeted delivery of 5-fluorouracil, miR-532-3p, and si-KRAS to the colorectal tumor using layer-by-layer liposomes

Spatiotemporal On–Off Immunomodulatory Hydrogel Targeting NLRP3 Inflammasome for the Treatment of Biofilm‐Infected Diabetic Wounds

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