Prolyl 4-hydroxylase subunit alpha 3 presents a cancer promotive function in head and neck squamous cell carcinoma via regulating epithelial-mesenchymal transition

Wang, Tao; Wang, Yongxin; Dong, Yan-Qin; Yu, Yanli; Ma, Ke

doi:10.1016/j.archoralbio.2020.104711

Cited by 21 publications

(15 citation statements)

References 32 publications

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“…Previous research showed that P4HA3 was up-regulated in head and neck squamous cell carcinoma (HNSCC) tissue, and it was demonstrated to promote HNSCC cell proliferation, invasion, and migration in vitro ( Wang T. et al, 2020 ). A recent study showed that the de-regulation of P4HA3 was associated with increased metastasis and poor prognosis of GC ( Song et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Diagnostic and Prognostic Biomarkers for Gastric Cancer Based on Bioinformatic Analysis

Niu

Ren²,

et al. 2022

Front. Genet.

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Background: Gastric cancer (GC) is one of the most prevalent cancers all over the world. The molecular mechanisms of GC remain unclear and not well understood. GC cases are majorly diagnosed at the late stage, resulting in a poor prognosis. Advances in molecular biology techniques allow us to get a better understanding of precise molecular mechanisms and enable us to identify the key genes in the carcinogenesis and progression of GC.Methods: The present study used datasets from the GEO database to screen differentially expressed genes (DEGs) between GC and normal gastric tissues. GO and KEGG enrichments were utilized to analyze the function of DEGs. The STRING database and Cytoscape software were applied to generate protein–protein network and find hub genes. The expression levels of hub genes were evaluated using data from the TCGA database. Survival analysis was conducted to evaluate the prognostic value of hub genes. The GEPIA database was involved to correlate key gene expressions with the pathological stage. Also, ROC curves were constructed to assess the diagnostic value of key genes.Results: A total of 607 DEGs were identified using three GEO datasets. GO analysis showed that the DEGs were mainly enriched in extracellular structure and matrix organization, collagen fibril organization, extracellular matrix (ECM), and integrin binding. KEGG enrichment was mainly enriched in protein digestion and absorption, ECM-receptor interaction, and focal adhesion. Fifteen genes were identified as hub genes, one of which was excluded for no significant expression between tumor and normal tissues. COL1A1, COL5A2, P4HA3, and SPARC showed high values in prognosis and diagnosis of GC.Conclusion: We suggest COL1A1, COL5A2, P4HA3, and SPARC as biomarkers for the diagnosis and prognosis of GC.

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Section: Discussionmentioning

confidence: 99%

Identification of Potential Diagnostic and Prognostic Biomarkers for Gastric Cancer Based on Bioinformatic Analysis

Niu

Ren²,

et al. 2022

Front. Genet.

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“…Prolyl 4-hydroxylase subunit alpha 3 (P4HA3) has been known by people that it has a relationship with many kinds of human cancers. There have been several studies indicating that P4HA3 is highly expressed, as an oncogene, activated in tumors as well as promoting EMT [ 23 , 24 , 42 ]. Here, our team demonstrated, for the first time, that miR-1266-3p could inhibit EMT by suppressing the expression of P4HA3.…”

Section: Discussionmentioning

confidence: 99%

“…The previous research found that the expression levels of both P4HA3 mRNA and protein of gastric cancer tissues were obviously increased compared with nongastric cancer tissues [ 22 ]. Also, P4HA3 manifested a cancer promoter through the regulation of EMT in the head and neck squamous cell cancer [ 23 ]. Additional study has indicated that P4HA3 and COL6A6 jointly restrain pituitary adenoma progression through obstructing PI3K-Akt signaling [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

miR-1266-3p Suppresses Epithelial-Mesenchymal Transition in Colon Cancer by Targeting P4HA3

Zhou

Huang

Shao

et al. 2022

Analytical Cellular Pathology

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Numerous studies have been conducted to demonstrate that miRNA is strongly related to colon cancer progression. Nevertheless, there are few studies regarding the function for miR-1266-3p in colon cancer, and the molecular mechanism remains poorly know. Our study was designed to examine the level of miR-1266-3p expression among the colon cancer tissue and cell and to study the role and regulatory mechanism for miR-1266-3p among colon cancer’s malignant biologic behavior. First, we found that miR-1266-3p expression was distinctly lower in colonic carcinoma tissues and cells than in nontumor ones, and the prognosis of low miR-1266-3p patients was distinctly worse than that of high miR-1266-3p patients. Second, we predicted that the target gene of miR-1266-3p was prolyl 4-hydroxylase subunit alpha 3 (P4HA3) through bioinformatics, and the targeting relationship between the two was verified by a dual luciferase assay report. Furthermore, miR-1266-3p inhibited the growth and metastasis of colon cancer in vitro as well as in vivo, and this effect could be alleviated by overexpressing P4HA3. Even more importantly, our study demonstrated that miR-1266-3p inhibited epithelial-mesenchymal transition (EMT) by targeting P4HA3. In conclusion, miR-1266-3p could inhibit growth, metastasis, and EMT in colon cancer by targeting P4HA3. Our discoveries might offer a novel target for colon cancer diagnosis and treatment.

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“…Unlike other amino acids, it shows smaller biological variation in CSF than in serum [29]. Post-translational modification of hydroxyproline is catalysed by the prolyl subunit of 4-hydroxylase alpha-3 (P4HA3), a key enzyme in collagen synthesis [30]. Increased accumulation of intracellular hydroxyproline and reduced procollagen synthesis in skin fibroblasts were found in patients harbouring cerebral aneurysms [31].…”

Section: Discussionmentioning

confidence: 99%

Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage

Bobeff

Bukowiecka‐Matusiak

Stawiski

et al. 2022

JCM

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Aneurysmal subarachnoid haemorrhages (aSAH) account for 5% of strokes and continues to place a great burden on patients and their families. Cerebral vasospasm (CVS) is one of the main causes of death after aSAH, and is usually diagnosed between day 3 and 14 after bleeding. Its pathogenesis remains poorly understood. To verify whether plasma concentration of amino acids have prognostic value in predicting CVS, we analysed data from 35 patients after aSAH (median age 55 years, IQR 39–62; 20 females, 57.1%), and 37 healthy volunteers (median age 50 years, IQR 38–56; 19 females, 51.4%). Fasting peripheral blood samples were collected on postoperative day one and seven. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis was performed. The results showed that plasma from patients after aSAH featured a distinctive amino acids concentration which was presented in both principal component analysis and direct comparison. No significant differences were noted between postoperative day one and seven. A total of 18 patients from the study group (51.4%) developed CVS. Hydroxyproline (AUC = 0.7042, 95%CI 0.5259–0.8826, p = 0.0248) and phenylalanine (AUC = 0.6944, 95%CI 0.5119–0.877, p = 0.0368) presented significant CVS prediction potential. Combining the Hunt-Hess Scale and plasma levels of hydroxyproline and phenylalanine provided the model with the best predictive performance and the lowest leave-one-out cross-validation of performance error. Our results suggest that plasma amino acids may improve sensitivity and specificity of Hunt-Hess scale in predicting CVS.

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Prolyl 4-hydroxylase subunit alpha 3 presents a cancer promotive function in head and neck squamous cell carcinoma via regulating epithelial-mesenchymal transition

Cited by 21 publications

References 32 publications

Identification of Potential Diagnostic and Prognostic Biomarkers for Gastric Cancer Based on Bioinformatic Analysis

Identification of Potential Diagnostic and Prognostic Biomarkers for Gastric Cancer Based on Bioinformatic Analysis

miR-1266-3p Suppresses Epithelial-Mesenchymal Transition in Colon Cancer by Targeting P4HA3

Plasma Amino Acids May Improve Prediction Accuracy of Cerebral Vasospasm after Aneurysmal Subarachnoid Haemorrhage

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