Promising alternatives for one-tier testing of Lyme borreliosis

Alasel, Mohammed; Keusgen, Michael

doi:10.1016/j.cca.2018.01.030

Cited by 7 publications

(8 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The direct test involves microscopic analysis, cultivation of Borrelia for 12 weeks in Barbour-Stoenner-Kelly medium and DNA sequences by PCR 11. The histopathological examination of the mitral valve obtained during the surgery from a patient with a confirmed diagnosis of Lyme endocarditis shows fibrin deposit and inflammatory infiltrate, and the presence of spirochetes 11. The indirect method is based on serological diagnosis, based on the detection of IgG and IgM antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…The indirect method is based on serological diagnosis, based on the detection of IgG and IgM antibodies. In humans, it takes 2–6 weeks after the vector bite for the body to produce such antibodies 11…”

Section: Discussionmentioning

confidence: 99%

“…The first test should be a sensitive ELISA for screening, followed by a western blot double-check. This two-tiered test has a specificity of 95%–100% and a sensitivity of >90% in the late stages of Lyme disease 11…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Lyme aortitis

et al. 2020

View full text Add to dashboard Cite

A 72-year-old man was admitted with complaints of sudden-onset oppressive precordial pain radiating to the back for 1 hour. He had hypotension, peripheral cyanosis and cold extremities. An initial assessment was done and acute coronary syndrome was excluded. After the patient was admitted, he developed fever and increased levels of inflammatory markers. Data obtained from CT angiography and transoesophageal echocardiogram revealed diffuse parietal thickening of the arch and the descending thoracic aorta, as well as dilatation of the aortic root and the proximal ascending aorta. In addition, the test for Borrelia burgdorferi was positive, and the patient was diagnosed with Lyme vasculitis of the thoracic aorta. He was treated with doxycycline for 3 weeks. Two months later, the patient exhibited a Stanford type A aortic dissection (clinically stable), which was treated by prosthesis replacement. The patient has remained asymptomatic for 1 year after the episode, performing his routine daily activities.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Lyme aortitis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Seroreactivity after successful treatment of Lyme borreliosis may persist for years [ 8 , 10 ]. Another diagnostic challenge is the possibility of false positive test results, possibly caused by cross-reactivity with other spirochaetal or bacterial antigens, as well as viral antigens, autoantigens [ 50 – 52 ]. Because using whole-cell lysates in ELISA is hindered by a greater possibility of cross-reactivity with other pathogenic bacteria, it is recommended that laboratories use at least a second generation ELISA test based on a recombinant antigen, which decreases the likelihood of cross-reactivity.…”

Section: Diagnostic Considerationsmentioning

confidence: 99%

Musculoskeletal manifestations of Lyme borreliosis – a review

Udziela¹,

Biesiada

Osiewicz

et al. 2020

Arch Med Sci

View full text Add to dashboard Cite

Lyme borreliosis (LB) is a zoonotic disease caused by Gram-negative bacteria Borrelia burgdorferi sensu lato. The majority of reported cases of LB originate in the northern hemisphere, mostly in the US and Europe. One of the typical manifestations of LB are musculoskeletal symptoms; they may appear in any of the three LB stages. The diagnosis is based on clinical manifestations and confirmed by serological tests. One course of antibiotic therapy is sufficient for LB to dissipate in most cases, although for some patients, the symptoms subside gradually even after completion of therapy. Patients who have been demonstrated to have specific antibodies but are symptomless should not be treated. In instances where the advised treatment proved to be unsuccessful, patients should be referred to rheumatologist for additional diagnostics. The goal of this review is to update physicians on current scientific knowledge on musculoskeletal manifestations of LB.

show abstract

“…Nucleic Acid Amplification Testing (NAAT), these methods remain inadequate due to the low concentration and transient presence of Bb in the blood. Culturing Bb is also not practical due the slow growth of the bacteria, as well as the need for specialized growth media [9][10][11] . Therefore, current testing methods work indirectly by detecting specific antibodies produced by the body's immune response to the infection.…”

Section: Introductionmentioning

confidence: 99%

Point-of-care serodiagnostic test for early-stage Lyme disease using a multiplexed paper-based immunoassay and machine learning

Joung

Ballard

et al. 2019

Preprint

View full text Add to dashboard Cite

Caused by the tick-borne spirochete, Borrelia burgdorferi, Lyme disease (LD) is the most common vector-borne infectious disease in North America and Europe. Though timely diagnosis and treatment are effective in preventing disease progression, current tests are insensitive in early-stage LD, with a sensitivity <50%. Additionally, the serological testing currently recommended by the US Center for Disease Control has high costs (>$400/test) and extended sample-to-answer timelines (>24 hours). To address these challenges, we created a cost-effective and rapid point-of-care (POC) test for early-stage LD that assays for antibodies specific to seven Borrelia antigens and a synthetic peptide in a paper-based multiplexed vertical flow assay (xVFA). We trained a deep learning-based diagnostic algorithm to select an optimal subset of antigen/peptide targets, and then blindly-tested our xVFA using human samples (N(+) = 42, N(-)= 54), achieving an area-under-the-curve (AUC), sensitivity, and specificity of 0.950, 90.5%, and 87.0% respectively, outperforming previous LD POC tests. With batch-specific standardization and threshold tuning, the specificity of our blind-testing performance improved to 96.3%, with an AUC and sensitivity of 0.963 and 85.7%, respectively.

show abstract

Promising alternatives for one-tier testing of Lyme borreliosis

Cited by 7 publications

References 54 publications

Lyme aortitis

Lyme aortitis

Musculoskeletal manifestations of Lyme borreliosis – a review

Point-of-care serodiagnostic test for early-stage Lyme disease using a multiplexed paper-based immunoassay and machine learning

Contact Info

Product

Resources

About