Promising results with 306 single blastocyst transfers

Single blastocyst transfer after ICSI from ejaculate spermatozoa, percutaneous epididymal sperm aspiration (PESA) or testicular sperm extraction (TESE)

“…However, if the theory that the selection of embryo is more adequate with blastocysts, than with day 2-3 embryos is true, a real difference between the two culture methods would be particularly apparent when only one embryo is transferred. Previously we [20], and others [21] have shown promising results with single blastocyst transfer. There is, however, only one randomised study that compared single day 2-3 and blastocyst transfer.…”

“…When preimplantation diagnostics is in clinical routine blastocysts in additionalso be more suitable to analyse than day 2-3 embryos. The evidence that blastocyst transfer yields a better outcome than day 2-3 transfer is conflicting [5,20]. However, if the theory that the selection of embryo is more adequate with blastocysts, than with day 2-3 embryos is true, a real difference between the two culture methods would be particularly apparent when only one embryo is transferred.…”

In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system

“…Blastocyst development is regulated by the expression of specific gene families that encode for cell polarity, cell junctional, cytoskeletal, ion transporter, and water channel gene products (10), and the degree of expansion of the human blastocyst is positively associated with pregnancy and implantation rates (11,12). Together, these observations suggest that culture in Global medium is more favorable for transcriptional activity and functional potential of the embryo compared with culture in ECM/Multiblast.…”

Top quality embryos at day 2: a prerequisite for single blastocyst transfer? An observational cohort study in women under 36

“…Extending culture to day 5/6 has been advocated to identify developmentally competent embryos for transfer or cryopreservation [4,5]. This approach should make possible the transfer of a single blastocyst, thereby maintaining high pregnancy rates while controlling the incidence of multiple gestations [6][7][8]. The integration of Values are means ± SD TQE top quality embryo blastocyst culture for all patients might virtually eliminate the incidence of high-order multiple pregnancies due to decrease in the number of embryos transferred [24].…”

“…The use of sequential media has demonstrated that extended embryo culture to the blastocyst stage enhances the likelihood of pregnancy [3][4][5]. Some studies have recently reported high pregnancy rates after single blastocyst transfer (SBT) on day 5 in selected couples [6][7][8].…”