Promoter Analysis for Three Types of EUL-Related Rice Lectins in Transgenic Arabidopsis

Atalah, Bassam Al; Fouquaert, Elke; Damme, Els J.M. Van

doi:10.1007/s11105-013-0611-2

Cited by 5 publications

(4 citation statements)

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“…Previous promoter analysis performed using the PLACE database (Al Atalah et al 2013 ) identified the presence of several TATA and CAAT boxes and identified three major classes of promoter elements, among which the light responsive elements, the ABA and GA responsive elements and the elements related to other (a)biotic stresses. There is some controversy about the significance of the TATA box for transcription initiation since analyses in A. thaliana (Molina and Grotewold 2005 ) and rice (Civan and Švec 2009 ) genomes revealed that only 29% of the Arabidopsis genes and 19% of the genes in O. sativa comprise a TATA box in their promoter region.…”

Section: Discussionmentioning

confidence: 99%

“…qRT-PCR experiments and microarray data revealed that transcript levels for some OsEULs are upregulated after infection of rice with Xanthomonas oryzae pv. oryzae (OsEULD1B, OsEULD2), Magnaporthe oryzae (OsEULD2) or root knot nematodes ( Meloidogyne graminicola ) (OsEULD2) (Al Atalah et al 2013 ; Kyndt et al 2012 ). In line with this, several WRKY transcription factors with binding sites identified in the OsEUL promoters are regulated by diverse biotic stresses.…”

Section: Discussionmentioning

confidence: 99%

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Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa)

Schutter

Tsaneva

Kulkarni

et al. 2017

Rice

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Background: Lectins, defined as 'Proteins that can recognize and bind specific carbohydrate structures', are widespread among all kingdoms of life and play an important role in various biological processes in the cell. Most plant lectins are involved in stress signaling and/or defense. The family of Euonymus-related lectins (EULs) represents a group of stress-related lectins composed of one or two EUL domains. The latter protein domain is unique in that it is ubiquitous in land plants, suggesting an important role for these proteins. Results: Despite the availability of multiple completely sequenced rice genomes, little is known on the occurrence of lectins in rice. We identified 329 putative lectin genes in the genome of Oryza sativa subsp. japonica belonging to nine out of 12 plant lectin families. In this paper, an in-depth molecular characterization of the EUL family of rice was performed. In addition, analyses of the promoter sequences and investigation of the transcript levels for these EUL genes enabled retrieval of important information related to the function and stress responsiveness of these lectins. Finally, a comparative analysis between rice cultivars and several monocot and dicot species revealed a high degree of sequence conservation within the EUL domain as well as in the domain organization of these lectins.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa)

Schutter

Tsaneva

Kulkarni

et al. 2017

Rice

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“…In rice, four types of EUL proteins have been identified (Fouquaert et al, 2009a;Fouquaert and Van Damme, 2012;Al Atalah et al, 2013). Two proteins with a single EUL domain preceded by an unrelated N-terminal sequence are classified as type S2 (OrysaEULS2) and type S3 (OrysaEULS3).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, OrysaEULD1A was regulated in the shoot tissues of rice seedlings infected with Magnaporthe oryzae and Xanthomonas oryzae (Li et al, 2006). Promoter activity analyses revealed that OrysaEULD1A might have a role in sensing and responding to external stresses as well as in the growth of the plant (Al Atalah et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a type D1A EUL-related lectin from rice expressed in Pichia pastoris

Atalah

Vanderschaeghe

Bloch

et al. 2013

Biological Chemistry

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OrysaEULD1A is one of the five EUL genes in rice (Oryza sativa) encoding a putative carbohydrate-binding protein belonging to the family of Euonymus related lectins (EUL). The OrysaEULD1A sequence comprises two highly similar EUL domains (91% sequence similarity and 72% sequence identity) separated by a 23 amino acid linker sequence and preceded by a 19 amino acid N-terminal sequence. In the present study, the full-length protein OrysaEULD1A as well as its individual domains OrysaEULD1A domain 1 and 2 were expressed in Pichia pastoris. After purification of the recombinant proteins, their carbohydrate-binding specificity was analyzed and compared. Interestingly, all recombinant lectins showed clear specificity towards galactosylated structures. Furthermore, all recombinant proteins agglutinated red blood cells, indicating that the full-length protein OrysaEULD1A and its domains are true lectins. These results taken together with data previously reported for single-domain EUL proteins indicate that although the amino acids--responsible for the formation of the carbohydrate-binding site--are identical for all EUL proteins in rice, these lectins show different carbohydrate specificities. This promiscuity of the carbohydrate-binding site can be attributed to gene divergence.

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Transcriptional behavior of EUL-related rice lectins toward important abiotic and biotic stresses

Atalah

Vleesschauwer

et al. 2014

Journal of Plant Physiology

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Promoter Analysis for Three Types of EUL-Related Rice Lectins in Transgenic Arabidopsis

Cited by 5 publications

References 49 publications

Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa)

Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa)

Characterization of a type D1A EUL-related lectin from rice expressed in Pichia pastoris

Transcriptional behavior of EUL-related rice lectins toward important abiotic and biotic stresses

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