Promoting osteoblast proliferation on polymer bone substitutes with bone-like structure by combining hydroxyapatite and bioactive glass

Zhao, Baisong; Xu, Haiping; Gao, Ying; Xu, Jia‐Zhuang; Yin, Hua-Mo; Xu, Ling; Li, Zhong‐Ming

doi:10.1016/j.msec.2018.11.006

Cited by 21 publications

(12 citation statements)

References 54 publications

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“…Alternatively, 2 Â 10 5 /ml cells were seeded in a 96-well plate (100 μl per well), 24-well plate (500 μl per well) or 6-well plate (2 ml) and cultured for another 24 h. Rat kindlin-1 coding sequence (GenBank accession: NM_001106515) was amplified using the following primers: forward 5 0 -CGCAAATGGG CGGTAGGCGT G-3 0 and reverse 5 0 -CATAGCGTAA AAGGAGCAAC A-3 0 . Cells were incubated with AAVkindlin-1 for 12 h to overexpress kindlin-1 as previously described (Zhao et al, 2019). Following media replacement, the cells were cultured for another 48 h and total protein or RNA was extracted and used for different assays.…”

Section: Regulation Of Kindlin-1 Expression By Shrna and Aav Transfec...mentioning

confidence: 99%

Wnt10a/β‐catenin signalling is involved in kindlin‐1‐mediated astrocyte activation in a chronic construction injury rat model

Zhao

Pan

2021

Eur J of Neuroscience

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The activation of spinal astrocytes and release of neuroinflammatory mediators are important events in neuropathic pain (NP) pathogenesis. In this study, we investigated the role of Wnt10a/β-catenin signalling in kindlin-1-mediated astrocyte activation using a chronic constriction injury (CCI) NP rat model. Using kindlin-1 overexpression and knockdown plasmids, we assessed hyperalgesia, changes in spinal astrocyte activation and the release of inflammatory mediators in a NP rat model. We also performed coimmunoprecipitation, Western blotting and real-time polymerase chain reaction (PCR) to characterize the underlying mechanisms of kindlin-1 in astrocyte cultures in vitro. Kindlin-1 was significantly upregulated in CCI rats and promoted hyperalgesia. Moreover, we observed increased kindlin-1, Wnt10a and glial fibrillary acidic protein (GFAP; biomarker for astroglial injury) levels and the release of inflammatory mediators in NP rats (p < 0.05). Inhibiting GFAP in vitro led to decreased kindlin-1 levels, prevented astrocyte activation, decreased Wnt10a level and the release of inflammatory mediators (p < 0.05). Coimmunoprecipitation showed that kindlin-1 can interact with Wnt10a. We showed that kindlin-1-mediated astrocyte activation was associated with Wnt10a/β-catenin signalling and the downstream release of inflammatory mediators in a CCI NP rat model. Our findings provide novel insights into the molecular mechanisms of kindlin-1-mediated astrocyte activation after CCI.

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Section: Regulation Of Kindlin-1 Expression By Shrna and Aav Transfec...mentioning

confidence: 99%

Wnt10a/β‐catenin signalling is involved in kindlin‐1‐mediated astrocyte activation in a chronic construction injury rat model

Zhao

Pan

2021

Eur J of Neuroscience

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“…Previous research demonstrated that nHA crystals with diameter of 40−100 nm were beneficial for promoting proliferation and differentiation. 24,25 Furthermore, BG could support osteogenesis via formation of the carbonate-substituted HA-like layer, which was analogous to the mineral component of bone. 26 It implies that the combination of nHA and BG is suitable for the construction of porous scaffolds.…”

Section: ■ Experimental Proceduresmentioning

confidence: 99%

Accelerating Bone Healing by Decorating BMP-2 on Porous Composite Scaffolds

Yin

Luo

et al. 2019

ACS Appl. Bio Mater.

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Although artificial polymeric scaffolds act as vital characters in bone repair, their application is limited due to their inferior bioactivity. Herein, osteoinductive poly(ε-caprolactone) (PCL) composite scaffolds were prepared by synchronously enlisting bioactive nanohydroxyapatite (nHA), bioglass (BG), and bone morphogenetic protein-2 (BMP-2), which was bound up with polydopamine (pDA). It was found that pDA deposition not only significantly enhanced hydrophilicity and cell affinity of composite scaffolds but also endowed steady immobilization of BMP-2 with long-term yet sustained release. Compared to pure PCL and PCL/nHA/BG (PHB) scaffolds, the designed PHB-pDA-BMP-2 scaffolds significantly induced the differentiation of bone marrow stromal cells toward an osteogenic lineage. Meanwhile, in vivo examinations revealed the prominent osteogenic capability of PHB-pDA-BMP-2 scaffolds, which enabled complete bone healing of calvarial bone defects in rabbits by 12 weeks. This finding indicates that the developed porous composite scaffolds hold great potential for bone regeneration.

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“…The immediate repair of bone disorders has been an ancestral clinical need, which has required the use of considerable resources in the field of medicine. Although many and various solutions have been successfully implemented, both internal and external treatment of bone defects remains a scientific challenge, as materials with adequate mechanical performance and favourable biological properties are required simultaneously [5]. However, with the current development of engineering-grade polymeric materials and the possibility of developing custom components by AM, new and versatile applications have been revealed for the biomechanical field in general, and orthopaedic in particular [6].…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, it has been shown that the imitation of the structural characteristics of human biological bone using substitute orthopaedic polymers has advantages for its implantation [5]. The anatomical complexity, details of the support structure, and variations in the densities, among other peculiarities, are characteristics that can be solved with the reproduction of a model by AM.…”

Section: Introductionmentioning

confidence: 99%

Mechanical Performance of 3D-Printed Biocompatible Polycarbonate for Biomechanical Applications

2021

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Additive manufacturing has experienced remarkable growth in recent years due to the customisation, precision, and cost savings compared to conventional manufacturing techniques. In parallel, materials with great potential have been developed, such as PC-ISO polycarbonate, which has biocompatibility certifications for use in the biomedical industry. However, many of these synthetic materials are not capable of meeting the mechanical stresses to which the biological structure of the human body is naturally subjected. In this study, an exhaustive characterisation of the PC-ISO was carried out, including an investigation on the influence of the printing parameters by fused filament fabrication on its mechanical behaviour. It was found that the effect of the combination of the printing parameters does not have a notable impact on the mass, cost, and manufacturing time of the specimens; however, it is relevant when determining the tensile, bending, shear, impact, and fatigue strengths. The best combinations for its application in biomechanics are proposed, and the need to combine PC-ISO with other materials to achieve the necessary strengths for functioning as a bone scaffold is demonstrated.

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Promoting osteoblast proliferation on polymer bone substitutes with bone-like structure by combining hydroxyapatite and bioactive glass

Cited by 21 publications

References 54 publications

Wnt10a/β‐catenin signalling is involved in kindlin‐1‐mediated astrocyte activation in a chronic construction injury rat model

Wnt10a/β‐catenin signalling is involved in kindlin‐1‐mediated astrocyte activation in a chronic construction injury rat model

Accelerating Bone Healing by Decorating BMP-2 on Porous Composite Scaffolds

Mechanical Performance of 3D-Printed Biocompatible Polycarbonate for Biomechanical Applications

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