Promoting osteoblasts responses in vitro and improving osteointegration in vivo through bioactive coating of nanosilicon nitride on polyetheretherketone

Abstract

Objective To enhance the bioactivity of polyetheretherketone (PEEK) while maintain its mechanical strengths. Methods Suspension coating and melt bonding. Results Silicon nitride (Si 3 N 4 , SN) coating lead to higher surface roughness, hydrophilicity and protein absorption; SN coating could slowly release Si ion into simulated body fluid (SBF), which caused weak alkaline of micro-environment owing to the slight dissolution of SN; SN coating resulted in the improvements of adhesion, proliferation, differentiation and gene expressions of MC3T3-E1 cells in vitro ; SN coating of PEEK with bioactive SN coating (CSNPK) obviously promoted bone regeneration and osseointegration in vivo . Conclusions CSNPK with SN coating as bone implant might be a promising candidate for orthopedic implants. The Translational Potential of this Article The silicon nitride-coated polyetheretherketone (CSNPK) prepared in this article could induce MC3T3-E1 cells adhesion, proliferation and differentiation in vitro ; it could also induce bone regeneration in bone defect in vivo , which indicate its good cytocompatibility and biocompatibility. If the raw materials are medical grade, and preparation process as well as production process of this article are further improved, it will have great translational potential.

A novel bioactive polyurethane with controlled degradation and L-Arg release used as strong adhesive tissue patch for hemostasis and promoting wound healing

“…To test and evaluate the biocompatibility of samples, we cultured further rat adipose stem cells on the G-DLPUs to observe the attachment and proliferation of cells [ [32] , [33] , [34] ]. All materials were treated in ultraviolet-sterilized biosafety cabinet before use.…”

Activity and Mechanism of Action of the Bioceramic Silicon Nitride as an Environmentally Friendly Alternative for the Control of the Grapevine Downy Mildew Pathogen Plasmopara viticola

“…In the attempt to find new alternatives that could be developed into effective, environmentally safe agrochemicals, we have investigated the ability of silicon nitride (Si 3 N 4 ), to knockdown P. viticola starting early in its infection cycle. The choice of this ceramic was based on its unique surface chemistry within an aqueous environment (Pezzotti, 2019) and its antibacterial (Pezzotti et al, 2018), antiviral (Pezzotti et al, 2020) and antifungal properties (Pezzotti, under review) while still being harmless to humans and widely used as a new therapeutic bioceramics in osteoarthropathy (Pezzotti et al, 2017;Dai et al, 2019;Calvert et al, 2020). Here we explore the possibility to consider Si 3 N 4 ) as a possible alternative for grapevine protection against P. viticola.…”

A microporous surface containing Si3N4/Ta microparticles of PEKK exhibits both antibacterial and osteogenic activity for inducing cellular response and improving osseointegration

“…The bacteria were generally negatively charged; thus, the interaction of negatively charged bacteria with positively charged –NH 3 + groups on SN could lead to disruption and lysis of the bacterial membrane [ 22 , 23 , 25 ]. Furthermore, the dissolution of SN could cause a weak alkaline microenvironment, which inhibited bacterial growth [ 25 , 28 , 37 ]. In this study, our results also demonstrated that both SNP and STP created a weak alkaline solution after soaking in SBF.…”