Proof of Concept for Recombinant Cellular Controls in Quantitative Molecular Pathogen Detection

Rossmanith, Peter; Mester, Patrick; Frühwirth, Karin; Fuchs, Sabine; Wagner, Martin

doi:10.1128/aem.02601-10

Cited by 12 publications

(11 citation statements)

References 25 publications

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“…However, for A1298C polymorphism, assessment of publication bias was useless and unnecessary because the number of the included studies is limited. (22), pancreatic cancer (23), gastric cancer (24), breast cancer (25), and hepatocellular cancer (26). On the contrary, 677T allele seems to reduce risk for colorectal cancer (27) and prostate cancer (28).…”

Section: Bias Diagnosticsmentioning

confidence: 89%

Polymorphisms of MTHFR C677T and A1298C Association With Oral Carcinoma Risk: A Meta-Analysis

Zhuo

Ling

Zhou

et al. 2012

Cancer Investigation

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Section: Bias Diagnosticsmentioning

confidence: 89%

Polymorphisms of MTHFR C677T and A1298C Association With Oral Carcinoma Risk: A Meta-Analysis

Zhuo

Ling

Zhou

et al. 2012

Cancer Investigation

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“…Accumulation of free DNA on frequently used door handles. Besides the monitoring of microbes in our proof-of-concept study, the prfA internal amplification control (IAC) assay (29) was examined in parallel because the lyophilized internal amplification control was accidently distributed in one room more than 10 years ago. Startlingly, this synthetic oligonucleotide of 100 bp could still be detected on the door handle of this room and even accumulated over time on the stickers, demonstrating the stability of DNA and the ability of the new sticker system to detect it effectively ( Fig.…”

Section: Resultsmentioning

confidence: 99%

A Novel Method for Sampling and Long-Term Monitoring of Microbes That Uses Stickers of Plain Paper

Bobal

Witte

Mester

et al. 2019

Appl Environ Microbiol

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Detection of pathogens is crucial in food production areas. While it is well established, swabbing as a state-of-the-art sampling method offers several drawbacks with respect to yield, standardization, overall handling, and long-term monitoring. This led us to develop and evaluate a method that is easier to use at a lower cost and that should be at least as sensitive. After evaluating sundry promising materials, we tested text-marking paper stickers for their suitability to take up and release Listeria monocytogenes with their nonsticky paper side over a 14-day time period using quantitative PCR. The recovery rate was similar to that in previous studies using conventional swabs, and we also confirmed the feasibility of pooling besides resilience to cleansing and disinfection. In a proof-of-concept experiment that sampled several locations, such as door handles, the occurrences of L. monocytogenes and Escherichia coli were determined. The results suggest that the presented sticker system might offer a promising cost-effective alternative sampling system with improved handling characteristics. IMPORTANCE As a ubiquitous bacterium, Listeria monocytogenes has a propensity to enter food production areas inadvertently via fomites such as door handles and switches. While the bacterium might not be in direct contact with the food products, knowing the microbial status of the surroundings is essential for risk assessment. Our investigation into a novel quantitative PCR (qPCR)-based sampling system with the highest sensitivity and ability to monitor over long periods of time, yet based on paper, proved to be cost-effective and reasonably convenient to handle.

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“…Either well‐defined wild or specifically designed engineered micro‐organisms may suit this purpose (Rossmanith et al . ). However, further development of this type of entire‐process controls for individual pathogens is necessary.…”

Section: Controlling the Analysismentioning

confidence: 97%

A decade with nucleic acid-based microbiological methods in safety control of foods

Kuchta

Knutsson

Fiore

et al. 2014

Lett Appl Microbiol

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In the last decade, nucleic acid-based methods gradually started to replace or complement the culture-based methods and immunochemical assays in routine laboratories involved in food control. In particular, real-time polymerase chain reaction (PCR) was technically developed to the stage of good speed, sensitivity and reproducibility, at minimized risk of carry-over contamination. Basic advantages provided by nucleic acid-based methods are higher speed and added information, such as subspecies identification, information on the presence of genes important for virulence or antibiotic resistance. Nucleic acid-based methods are attractive also to detect important foodborne pathogens for which no classical counterparts are available, namely foodborne pathogenic viruses. This review briefly summarizes currently available or developing molecular technologies that may be candidates for involvement in microbiological molecular methods in the next decade. Potential of nonamplification as well as amplification methods is discussed, including fluorescent in situ hybridization, alternative PCR chemistries, alternative amplification technologies, digital PCR and nanotechnologies.

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Proof of Concept for Recombinant Cellular Controls in Quantitative Molecular Pathogen Detection

Cited by 12 publications

References 25 publications

Polymorphisms of MTHFR C677T and A1298C Association With Oral Carcinoma Risk: A Meta-Analysis

Polymorphisms of MTHFR C677T and A1298C Association With Oral Carcinoma Risk: A Meta-Analysis

A Novel Method for Sampling and Long-Term Monitoring of Microbes That Uses Stickers of Plain Paper

A decade with nucleic acid-based microbiological methods in safety control of foods

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