Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira

Borghezan, Marcelo; Moraes, Liziane Kadine Antunes de; Moreira, Fábio Mosso; Silva, Aparecido Lima da

doi:10.1590/s0100-204x2003000700001

Cited by 11 publications

(17 citation statements)

References 13 publications

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“…When comparing MS [22] and Roubelakis' formulations in micropropagation of 15 vine cultivars, Roubelakis-Angelakis and Zivanovitc [13] noted better development and greater formation of primary roots per culture with materials cultivated in Roubelakis' environment. Results were similar to those found by Borghezan et al [23], who obtained, after 60 days of in vitro vine rootstocks cultivation, the greatest value of root length from 9.0 cm to 10.5 cm.…”

Section: Resultssupporting

confidence: 92%

“…Values were higher than those related by Borghezan et al [23] with SO4 and Paulsen 1103 vine rootstocks, which after 60 days of in vitro cultivation in DSD1 formula showed 34.8 mg and 35.6 mg dry mass accumulation, respectively.…”

Section: Resultscontrasting

confidence: 72%

“…Similarly, Borghezan et al [23] observed that acclimatized plants from VR043-43, VR039-16 and Paulsen 1103 vine rootstocks showed lower chlorophyll content in relation to in vitro plants; authors have highlighted those plants are in an adjustment process to new environment conditions. After 60 days of acclimatization, the accumulation of dry biomass was bigger for Poloskei Muskotaly plants cultivated in vitro in Roubelakis, ZL and C2D formulas in relation to DSD1 and Galzy.…”

Section: Resultsmentioning

confidence: 91%

“…Although not the best formula for root dry mass variable, Poloskei Muskotaly cultivated with DSD1 is between the limits of 2.7 mg and 7.6 mg of dry root as defined by Borghezan et al [23] who evaluated six vine rootstocks in DSD1 formula.…”

Section: Resultsmentioning

confidence: 99%

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<i>In Vitro</i> Propagation of Grapevine Cultivars with Potential for South of Brazil

Bettoni¹,

Costa²,

Gardin³

et al. 2015

AJPS

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The micropropation is an important biotechnological tool for obtaining and maintaining mother vine plants with high quality plant health. The objective was to evaluate the establishment and multiplication in vitro and ex vitro acclimatization of grape genotypes with potential for Southern Brazil. Vine nodal segments were cultured in five culture medium formulations without adding growth regulators. It was evaluated the number of leaves and roots, length of roots and shoots, replication rate, relative chlorophyll index, percentage of regenerated and rooted plants, dry biomass of shoot, root and total plants grown in vitro and after acclimatization. In vitro propagation of IAC 571-6 rootstock and cv. Poloskei Muskotaly through nodal segments provided high rates of regeneration and rooting. High survival rates were obtained in the acclimatization of IAC 571-6 and Pölöskei Muskotaly. Considering all the variables, the culture medium Roubelakis showed the best growth rates and development for shoots and roots, and in vitro multiplication rate for IAC 571-6 and Poloskei Muskotaly grape varieties.

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Section: Resultssupporting

confidence: 92%

Section: Resultscontrasting

confidence: 72%

Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

<i>In Vitro</i> Propagation of Grapevine Cultivars with Potential for South of Brazil

Bettoni¹,

Costa²,

Gardin³

et al. 2015

AJPS

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“…Faisal and Anis (2009), working with Rauvolfia tetraphylla found increases in chlorophylls a and b in young leaves developed after acclimatization. Borghezan et al (2003), studying some genotypes of grapevine (Vitis spp. ), observed in two of them a higher total chlorophylls content in plantlets from in vitro tissues than from acclimatized plantlets.…”

Section: Discussionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

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Biostimulants and indolebutyric acid improve rooting of wood cuttings from different grapevine rootstocks

Brighenti

Freitas

Malohlava

et al. 2023

Ciência Téc. Vitiv.

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The rooting of rootstocks is considered a critical point in the production of high-quality grapevine planting material. To maximize the cutting rhizogenesis process, plant hormones, such as auxins, are used for the development of adventitious roots. In addition, some groups of biostimulant have as their main role the development of root system, ensuring good growth of the plant aerial part. However, research using algae extract-based biostimulants in vegetative propagation of vine rootstocks is scarce. In this sense, the objective of this work was to evaluate the use of the biostimulant based on algae extract and indolebutyric acid (IBA), in the development of the root system and vegetative growth of grapevine rootstocks. The rootstocks evaluated were ‘3309 C’, ‘101-14 Mgt’, ‘Paulsen 1103’, ‘99 R’, ‘110 R’, ‘Kober 5BB’, ‘SO4’, ‘420A Mgt’, ‘Solferino’, ‘Gravesac’, ‘IAC 572’, ‘IAC 766’, ‘IAC 313’, ‘Harmony’, ‘Freedom’, ‘Salt Creek’ and ‘VR 043- 43’. The experimental design was completely randomized with four replications, arranged in a factorial scheme, involving 17 rootstocks associated with four treatments to stimulate rooting (IBA, algae extract, IBA + algae extract and control). The use of indolebutyric acid and algae extract-based biostimulant proved to be efficient in rooting woody cuttings from different vine rootstocks. The use of IBA, algae extract-based biostimulant and IBA combined with algae extract resulted in higher rates of rooting and development than the control. The rootstocks presenting the highest rooting rates and vegetative development were ‘IAC 572’ and ‘Freedom’. ‘Gravesac’, ‘101-14 Mgt’ and ‘99 R’, showing high rates of rooting and root development, but low vegetative development. The rootstocks exhibiting the lowest rooting rates and vegetative development were ‘Salt Creek’, ‘VR 043-43’ and ‘110 R’.

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Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira

Cited by 11 publications

References 13 publications

<i>In Vitro</i> Propagation of Grapevine Cultivars with Potential for South of Brazil

<i>In Vitro</i> Propagation of Grapevine Cultivars with Potential for South of Brazil

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Biostimulants and indolebutyric acid improve rooting of wood cuttings from different grapevine rootstocks

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Propagação in vitro e avaliação de parâmetros morfofisiológicos de porta-enxertos de videira

Cited by 11 publications

References 13 publications

&lt;i&gt;In Vitro&lt;/i&gt; Propagation of Grapevine Cultivars with Potential for South of Brazil

&lt;i&gt;In Vitro&lt;/i&gt; Propagation of Grapevine Cultivars with Potential for South of Brazil

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Biostimulants and indolebutyric acid improve rooting of wood cuttings from different grapevine rootstocks

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<i>In Vitro</i> Propagation of Grapevine Cultivars with Potential for South of Brazil

<i>In Vitro</i> Propagation of Grapevine Cultivars with Potential for South of Brazil