Propamidine decreas mitochondrial complex III activity of Botrytis cinerea

Wu, Fuyong; Jin, Weibo; Feng, Juntao; Chen, Anliang; Ma, Zhiqing; Zhang, Xing

doi:10.5483/bmbrep.2010.43.9.614

Cited by 7 publications

(8 citation statements)

References 22 publications

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“…It was presumed that compound SCU2028 might lead to the rupture of the cell wall or cell membrane, nutrient leakage, and death . At the same time, the abnormal mitochondrial expression indicated that compound SCU2028 had some effects on the fungus’ respiration or energy synthesis. , …”

Section: Resultsmentioning

confidence: 99%

“…20 At the same time, the abnormal mitochondrial expression indicated that compound SCU2028 had some effects on the fungus' respiration or energy synthesis. 21,22 Effects on the MMP. Fluorescent dye rhodamine 123 can directly distribute in the mitochondrial matrix according to the intensity of the MMP, and its fluorescence intensity can reflect the change of the MMP.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Mechanism of Action of Novel Pyrazole Carboxamide Containing a Diarylamine Scaffold against Rhizoctonia solani

Zhao

Yang

Deng

et al. 2020

J. Agric. Food Chem.

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In the last few decades, Rhizoctonia solani causing rice sheath blight has resulted in a lot of economic losses in the world. Therefore, many novel pyrazole carboxamide fungicides have been intensively researched and employed to fight against it. In this regard, in recent years, our group reported a novel pyrazole carboxamide containing a diarylamine scaffold with good antifungal activity against rice sheath blight in the pot test and field trial. Following this project, the antifungal mechanism of action of the pyrazole carboxamide has been elucidated in this work. The antifungal result showed that compound SCU2028, N-[2-[(3chlorophenyl)amino]-phenyl]-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide, was equivalent to the commercial fungicide thifluzamide and its EC 50 value was 0.022 mg/L against R. solani. Also, the observation results by scanning electron microscopy and transmission electron microscopy showed that it could destroy the fungus' cell walls or membranes and result in the leakage of contents and increase of the number of mitochondria and abnormal morphology. Meanwhile, the result on the mitochondrial membrane potential (MMP) showed that it could decrease R. solani's MMP. Furthermore, the results by label-free quantitative proteomic analysis showed that 1153 proteins were found after R. solani was treated with compound SCU2028, including 212 proteins in the control group and 257 proteins in the treatment group. A total of 142 differential proteins were obtained, of which 92 proteins were upregulated and 50 proteins were downregulated. The differentially expressed proteins affected a series of physiological and biochemical pathways in the mitochondria, endoplasmic reticulum, ribosome, and other related GO and KEGG pathways. In particular, the inhibition of the respiratory chain caused by the TCA cycle and oxidative phosphorylation KEGG pathway indicated that complex II (succinate dehydrogenase) and complex IV (cytochrome oxidase) might be compound SCU2028's main action targets. In addition, multiple experiments of qRT-PCR, enzyme activity detection, and molecular docking confirmed complex II and complex IV as targets. It could be seen that these findings provided a theoretical support for further research and development of the pyrazole carboxamide fungicides.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Mechanism of Action of Novel Pyrazole Carboxamide Containing a Diarylamine Scaffold against Rhizoctonia solani

Zhao

Yang

Deng

et al. 2020

J. Agric. Food Chem.

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“…40 Because of its ability to disrupt mitochondrial membranes, 1 can be used as an antimicrobial agent too. 41 Despite such wide applicability, the use of diamidines for PRMT inhibition has not been reported previously except for stilbamidine. We studied a number of diamidine compounds for PRMT inhibition, and several showed clear selectivity for PRMT1.…”

Section: Introductionmentioning

confidence: 99%

Diamidine Compounds for Selective Inhibition of Protein Arginine Methyltransferase 1

et al. 2014

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Protein arginine methylation is a posttranslational modification critical for a variety of biological processes. Misregulation of protein arginine methyltransferases (PRMTs) has been linked to many pathological conditions. Most current PRMT inhibitors display limited specificity and selectivity, indiscriminately targeting many methyltransferase enzymes that use S-adenosyl-l-methionine as a cofactor. Here we report diamidine compounds for specific inhibition of PRMT1, the primary type I enzyme. Docking, molecular dynamics, and MM/PBSA analysis together with biochemical assays were conducted to understand the binding modes of these inhibitors and the molecular basis of selective inhibition for PRMT1. Our data suggest that 2,5-bis(4-amidinophenyl)furan (1, furamidine, DB75), one leading inhibitor, targets the enzyme active site and is primarily competitive with the substrate and noncompetitive toward the cofactor. Furthermore, cellular studies revealed that 1 is cell membrane permeable and effectively inhibits intracellular PRMT1 activity and blocks cell proliferation in leukemia cell lines with different genetic lesions.

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“…For single immunoflorescence labeling, the sections were stained with rabbit anti-collagen IV (1：80, chemicon, USA) overnight at 4℃ and stained again with secondary antibody Alexa 488 donkey anti-rabbit IgG (1：400, Invitrogen, USA) at room temperature for 1 hour (27). The sections were mounted by aqueous mounting medium (DAKO, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Ultrathin sections (approximately 60-70 nm thick) were prepared by ultramicrotome and were stained with uranyl acetate in 30% ethanol for 10 minutes and 0.03% lead citrate in 0.01 N natrium hydroxide for 10 minutes. The sections were washed in tap water and were allowed to dry at room temperate (28). They were evaluated by transmission electron microscope (H-7600 operated at 80 kW, Hitachi Co., Japan) and photographed.…”

Section: Methodsmentioning

confidence: 99%

Accelerating repaired basement membrane after bevacizumab treatment on alkali-burned mouse cornea

Lee

Lee²,

Lee³

et al. 2013

BMB Reports

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To understand the corneal regeneration induced by bevacizumab, we investigated the structure changes of stroma and basement membrane regeneration. A Stick soaked in 0.5 N NaOH onto the mouse cornea and 2.5 mg/ml of bevacizumab was delivered into an alkali-burned cornea (2 μl) by subconjunctival injections at 1 hour and 4 days after injury. At 7 days after injury, basement membrane regeneration was observed by transmission electron microscope. Uneven and thin epithelial basement membrane, light density of hemidesmosomes, and edematous collagen fibril bundles are shown in the alkali-burned cornea. Injured epithelial basement membrane and hemidesmosomes and edematous collagen fibril bundles resulting from alkali-burned mouse cornea was repaired by bevacizumab treatment. This study demonstrates that bevacizumab can play an important role in wound healing in the cornea by accelerating the reestablishment of basement membrane integrity that leads to barriers for scar formation. [BMB Reports 2013; 46(4): 195-200]

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Propamidine decreas mitochondrial complex III activity of Botrytis cinerea

Cited by 7 publications

References 22 publications

Mechanism of Action of Novel Pyrazole Carboxamide Containing a Diarylamine Scaffold against Rhizoctonia solani

Mechanism of Action of Novel Pyrazole Carboxamide Containing a Diarylamine Scaffold against Rhizoctonia solani

Diamidine Compounds for Selective Inhibition of Protein Arginine Methyltransferase 1

Accelerating repaired basement membrane after bevacizumab treatment on alkali-burned mouse cornea

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