Propanol – Sugar aqueous biphasic systems as a suitable platform for biomolecules extraction

Ebrahimi, Nosaibah; Sadeghi, Rahmat

doi:10.1016/j.chroma.2018.11.007

Cited by 30 publications

(18 citation statements)

References 57 publications

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“…The clouding (liquid–liquid demixing) phase diagrams for ternary systems of 1-butanol in aqueous amino acid/IL/poly(ethylene glycol) (PEG)/propanol solutions were determined by the visual turbidity titration method. The methods’ procedure and apparatus details have been explained in our previous works. ,, Here, to determine 1-butanol mass fraction corresponding to the clouding phenomenon at each temperature (283.1–353.1 K with an increase of 10 K), binary aqueous solutions of the investigated additives {amino acids (0.2, 0.4, and 0.6 mol·kg –1 ), ILs (0.2, 0.4, and 0.6 mol·kg –1 ), polymers (0.05, 0.10, and 0.15 w/w), and 1-/2-propanol (0.05, 0.10, and 0.15 w/w)} were first prepared and poured into a transparent double-walled glass cell. Then, pure 1-butanol was dripped on each binary limpid solution until a cloudy mixture was detected at each temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The previous studies have shown that infinite dilution activity coefficients of C1−C4 alcohols follows the trend 1-butanol> 2-butanol > 1-propanol > 2-propanol > ethanol > methanol, indicating the lower hydrophilicity of primary alcohols, compared to the secondary alcohols, for the same numbers of carbon atoms. 27,51,52 Moreover, it has been proved that more hydrophilic sugars are needed to solute out 2-propanol and form sugar + 2-propanol ABSs, as compared with that to form sugar + 1-propanol ABSs, confirming that 2-propanol is more hydrophilic than 1propanol. 27 Therefore, it can be said that because of its more hydrophilic nature, 2-propanol acts more successfully as a bridge between water and 1-butanol and more effectively reconciles 1butanol with water, as compared with 1-propanol.…”

Section: T H I S C O N T E N T Imentioning

confidence: 97%

“…27,51,52 Moreover, it has been proved that more hydrophilic sugars are needed to solute out 2-propanol and form sugar + 2-propanol ABSs, as compared with that to form sugar + 1-propanol ABSs, confirming that 2-propanol is more hydrophilic than 1propanol. 27 Therefore, it can be said that because of its more hydrophilic nature, 2-propanol acts more successfully as a bridge between water and 1-butanol and more effectively reconciles 1butanol with water, as compared with 1-propanol. The obtained results reveal that the hydrophobic interactions overcome the hydrophilic interactions and increase the biphasic region area at low temperatures.…”

Section: T H I S C O N T E N T Imentioning

confidence: 97%

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Soluting-In, Soluting-Out, and Washing-Out Effects of Various Additives on Aqueous 1-Butanol Solutions

2021

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This work addresses the liquid−liquid demixing (clouding) behavior of 1-butanol aqueous solutions in the presence of various additives, including amino acids, imidazolium-based ionic liquids (ILs), polymers, and 1-/2-propanol, in a wide temperature range (283.1−353.1 K) and at atmospheric pressure (845 hPa). The visual cloud point data revealed that the studied amino acids reduce the water solubility of 1-butanol and expand the immiscibility region (soluting-out effect). At the same amino acid's molality, the soluting-out aptitude follows the trend serine > glycine > alanine > proline > glutamine. However, the ILs and polymers [poly(ethylene glycol) (PEG)] act as cosolvents for 1butanol in aqueous media and broaden the miscibility region (soluting-in effect). An increase in the cation alkyl chain length of ILs and a decrease in PEG molar mass strengthen the soluting-in effect. The clouding phase behavior of aqueous 1-butanol + 1-/2propanol ternary systems at lower temperatures reveals the expansion of the immiscibility region due to the washing-out effect but at higher temperatures exhibits the soluting-in effect. An increase in the concentration of 1-/2-propanol decreases the extent of the washing-out effect. In all cases, the soluting-in/out effects increase with the increase in the concentration of additives. Soluting effects occurring in the studied systems have been quantified by correlating the experimental cloud point data with Setschenow's equation, and the clouding process in the systems studied has been carefully discussed from the thermodynamic point of view. From the gathered results, at higher temperatures, the relative contribution of enthalpy to clouding Gibbs free energy in the presence of soluting-in agent additives is mostly higher than that in the presence of soluting-out agents. However, at lower temperatures, the entropy increase is the driving force of clouding in most of the investigated systems.

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Section: Methodsmentioning

confidence: 99%

Section: T H I S C O N T E N T Imentioning

confidence: 97%

Section: T H I S C O N T E N T Imentioning

confidence: 97%

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Soluting-In, Soluting-Out, and Washing-Out Effects of Various Additives on Aqueous 1-Butanol Solutions

2021

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“…The trend was in agreement with the sugaring-out ability and hydrophilicity of the sugars investigated. It has been reported that the ability of these sugars in sugaring-out ionic liquids (Ferreira et al 2016), polymers (Sadeghi et al 2016), and alcohols (Ebrahimi and Sadeghi 2018) for the formation of ABS increased in the order of glucose < sucrose < maltose. As shown in Fig.…”

Section: Binodal Curves Of Sugaring-out Assisted Absmentioning

confidence: 99%

Efficacy of alcohol/sugar aqueous biphasic system on partition of bovine serum albumin

Chow

Sahlini

et al. 2021

Bioresour. Bioprocess.

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The efficacy of alcohol/sugar aqueous biphasic (ABS) system on protein extraction was investigated. A model protein, bovine serum albumin (BSA), was adopted to evaluate the effects of types and concentration of phase-forming components, protein concentration, and system pH on the protein partition efficiency. The 1-propanol/maltose ABS exhibited an overall better partition efficiency of BSA to the alcohol-rich top phase. A maximum partition coefficient (K) of 20.01 ± 0.05 and recovery yield (Y) of 95.42% ± 0.01% of BSA were achieved with 35% (w/w) 1-propanol/22% (w/w) maltose ABS at pH 5.0 for 10% (w/w) BSA load. The K and Y of BSA in 1-propanol/maltose ABS was slightly improved with the addition of 3% (w/w) of ionic liquid, 1-butyl-3-methylimidazolium bromide ([Bmim]Br) as the adjuvant that could provide protein stabilizing effect. The Fourier Transform Infrared Spectrum (FTIR) analysis revealed that the protein structure remained unaltered upon the separation process.

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“…Sugars possess several hydroxyl groups with dual donor/acceptor characteristics that can participate in hydrogen bonding, thereby exerting a sugaring-out effect (also known as soluting-out effect) inherently (de Brito Cardoso et al 2013). Rising attention has been given to the application of carbohydrates as a sugaring-out agent to form polymer/sugar ABS (Sadeghi et al 2016), acetonitrile/sugar ABS (de Brito Cardoso et al 2013), ionic liquid (IL)/sugar ABS (Quental et al 2018) and alcohol/sugar ABS (Ebrahimi and Sadeghi 2018). The viscosity of alcohol is lower when compared to polymers and ILs, allowing more rapid phase separation and e cient mass transfer of biomolecules from one phase to another (Wang et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Alcohol/sugar Aqueous Biphasic System on Partition of Bovine Serum Albumin

Chow

Sahlini

et al. 2020

Preprint

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A green bio-separation alternative can be performed with a non-toxic and biodegradable aqueous biphasic system (ABS) composed of short-chain aliphatic alcohol-based top phase (1-propanol and 2-propanol) and carbohydrate-based bottom phase (glucose, sucrose, and maltose). A model protein, bovine serum albumin (BSA) was adopted to determine the effects of types and concentration of phase-forming components; protein concentration; and system pH on the protein partition efficiency in the ABS. Results showed that the 1-propanol/maltose ABS gave an overall better partition efficiency of BSA to the alcohol-rich top phase compared to the 1-propanol/sucrose ABS, 1-propanol/glucose ABS, and 2-propanol/sugar ABS attributed to the lower hydrophilicity of 1-propanol and the stronger sugaring-out effect exerted by the maltose. A maximum partition coefficient (K) of 20.01 ± 0.05 and recovery yield (Y) of 95.42% ± 0.01 of BSA were obtained with the 35% (w/w) 1-propanol/22% (w/w) maltose ABS at pH 5.0 which contained 10% (w/w) BSA. The K and Y of BSA in 1-propanol/maltose ABS was further enhanced with the addition of 3% (w/w) of ionic liquids, 1-butyl-3-methylimidazolium bromide ([Bmim]Br) as the adjuvants which provides the protein stabilizing effect. The Fourier Transform Infrared Spectrum (FTIR) analysis revealed that the protein structure remained unaltered upon the separation process.

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Propanol – Sugar aqueous biphasic systems as a suitable platform for biomolecules extraction

Cited by 30 publications

References 57 publications

Soluting-In, Soluting-Out, and Washing-Out Effects of Various Additives on Aqueous 1-Butanol Solutions

Soluting-In, Soluting-Out, and Washing-Out Effects of Various Additives on Aqueous 1-Butanol Solutions

Efficacy of alcohol/sugar aqueous biphasic system on partition of bovine serum albumin

Efficacy of Alcohol/sugar Aqueous Biphasic System on Partition of Bovine Serum Albumin

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