Properties of surface functionalized iron oxide nanoparticles (ferrofluid) conjugated antibody for lateral flow immunoassay application

Nor, Noorhashimah Mohamad; Razak, Khairunisak Abdul; Tan, Soo Choon; Noordin, Rahmah

doi:10.1016/j.jallcom.2012.05.053

Cited by 42 publications

(10 citation statements)

References 28 publications

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“…However, one of the major disadvantages of SPIONs is the agglomeration of the particles owing to their high surface energies [19] , [20] . To overcome this limitation, surface modifications of SPIONs with biocompatible materials are one of the reported strategies [21] , [22] , [23] , [24] , [25] . Chitosan (CS) is a natural biopolymer with comprehensive biological and chemical properties which has been used to coat the surface of SPIONs [26] , [27] .…”

Section: Introductionmentioning

confidence: 99%

Ultrasound assisted chitosan coated iron oxide nanoparticles: Influence of ultrasonic irradiation on the crystallinity, stability, toxicity and magnetization of the functionalized nanoparticles

Braim

Razak

Aziz

et al. 2022

Ultrasonics Sonochemistry

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Section: Introductionmentioning

confidence: 99%

Ultrasound assisted chitosan coated iron oxide nanoparticles: Influence of ultrasonic irradiation on the crystallinity, stability, toxicity and magnetization of the functionalized nanoparticles

Braim

Razak

Aziz

et al. 2022

Ultrasonics Sonochemistry

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“…The iron oxide nanoparticles (Fe 3 O 4 ) obtained were spherical. Other authors using similar methods also produced nanoparticles of the same morphology 24,25,30,31 . This morphology is related to the nucleation rate per unit area that is isotropic at the nanoparticle interface.…”

Section: Resultsmentioning

confidence: 99%

“…This morphology is related to the nucleation rate per unit area that is isotropic at the nanoparticle interface. Nucleation starts when the iron II concentration supersaturates and continues with the nucleus growth by the diffusion and absorption of solutes from the solution to the surface until the equilibrium concentration in the medium is reached 25,31 …”

Section: Resultsmentioning

confidence: 99%

Development of modified graphite electrodes with nanoparticles and their application in the quantification of sucrose

Santos

Ribeiro

Oliveira

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND Developing fast and reliable methods with little sample preparation has become increasingly important for process monitoring and control. Electrochemical methods stand out for their low cost and instrumental simplicity. Furthermore, electrodes with nanoparticles can improve the method's sensitivity and specificity. This study developed carbon paste electrodes modified with nanoparticles of copper and iron. In addition, cyclic voltammetry was performed using a low‐cost potentiostat designed for a Raspberry Pi single‐board computer. As a proof of concept, the constructed electrodes were tested with ferrocyanide and applied to quantify sucrose, and their performance was compared to that of the glassy carbon electrode. RESULTS The nanoparticles were successfully synthesized, as confirmed by morphology and physicochemical characterization. Based on the adjusted R2, the electrode made with the addition of copper oxide nanoparticles has a similar performance to the commercial glassy carbon electrode. All electrodes' root mean square error (RMSE) values were approximately 10−4 mol L−1. The lowest values for the inverse of the analytical sensitivity for sucrose were obtained for the unmodified carbon paste electrode (9.54 × 10−6 mol L−1) and copper oxide nanoparticles electrode (5.30 × 10−6 mol L−1). These electrodes also had the lowest limits of detection (10−5 mol L−1) and, for the limit of quantification, all electrodes had similar values (10−2 mol L−1). CONCLUSION In general, superior performance of carbon paste electrodes was observed concerning the commercial glassy carbon electrodes. Compared to previous studies, the developed electrodes showed good sensitivity and the low cost of the electrochemical system stands out as the main advantage. © 2022 Society of Chemical Industry (SCI).

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“…Therefore, electrostatic and steric repulsion need to be created between SPIONs to prevent agglomeration and produce a stable nanoparticle. It was also demonstrated that peptization prevents agglomeration, paving the way for the production of stabilized magnetic nanoparticles [ 60 ]. The antibody conjugation may stabilize the particles further, preventing aggregation and hence reducing the hydrodynamic size [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

Khodadust¹,

Ünal²,

Acar³

2022

Beilstein J. Nanotechnol.

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Polyethylenimine (PEI), which is frequently used for polyplex formation and effective gene transfection, is rarely recognized as a luminescent polymer. Therefore, it is usually tagged with an organic fluorophore to be optically tracked. Recently, we developed branched PEI (bPEI) superparamagnetic iron oxide nanoparticles (SPION@bPEI) with blue luminescence 1200 times stronger than that of bPEI without a traditional fluorophore, due to partial PEI oxidation during the synthesis. Here, we demonstrate in vitro dye-free optical imaging and successful gene transfection with luminescent SPION@bPEI, which was further modified for receptor-mediated delivery of the cargo selectively to cancer cell lines overexpressing the epidermal growth factor receptor (EGFR). Pro-apoptotic polyinosinic–polycytidylic acid sodium (PIC) was delivered to HeLa cells with SPION@bPEI and caused a dramatic reduction in the cell viability at otherwise non-toxic nanoparticle concentrations, proving that bPEI coating is still an effective component for the delivery of an anionic cargo. Besides, a strong intracellular optical signal supports the optically traceable nature of these nanoparticles. SPION@bPEI nanoparticles were further conjugated with Erbitux (Erb), which is an anti-EGFR antibody for targeting EGFR-overexpressing cancer cell lines. SPION@bPEI-Erb was used for the delivery of a GFP plasmid wherein the transfection was confirmed by the luminescence of the expressed gene within the transfected cells. Poor GFP expression in MCF7, a slightly better expression in HeLa, and a significant enhancement in the transfection of HCT116 cells proved a selective uptake and hence the targeting ability of Erb-tagged nanoparticles. Altogether, this study proves luminescent, cationic, and small SPION@bPEI nanoparticles as strong candidates for imaging and gene therapy.

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Properties of surface functionalized iron oxide nanoparticles (ferrofluid) conjugated antibody for lateral flow immunoassay application

Cited by 42 publications

References 28 publications

Ultrasound assisted chitosan coated iron oxide nanoparticles: Influence of ultrasonic irradiation on the crystallinity, stability, toxicity and magnetization of the functionalized nanoparticles

Ultrasound assisted chitosan coated iron oxide nanoparticles: Influence of ultrasonic irradiation on the crystallinity, stability, toxicity and magnetization of the functionalized nanoparticles

Development of modified graphite electrodes with nanoparticles and their application in the quantification of sucrose

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

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