Properties of Vasoactive Intestinal Polypeptide Receptors in the Anterior Pituitary of Female Chickens.

Gonzales, Sylvia Mae T.; Hawashima, Mitsuo; Kamayoshi, Michiharu; Tanaka, Katsuhide; Ichinoe, Kenji

doi:10.1262/jrd.40.213

Cited by 3 publications

(6 citation statements)

References 34 publications

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“…The binding site was of a single class. The value of K , is almost the same as that reported on the chicken pineal (23), hypothalamus (16), and pituitary (15); the rat brain (24); and the cat renal outer medulla (25). However, a different value was reported on the chicken thymus and bursa of fabricius (26), the guinea pig brain (27); the rat brain (28), pineal (29), blood vessels (30), and uterus (31); and the feline kidney (25).…”

Section: Discussionsupporting

confidence: 83%

“…cVIP (Applied Biosystem, San Francisco, CA) was labeled with NA-'251 (Amersham International, Buckinghamshire, United Kingdom) using the iodogen method (15,16) and chromatographed on a 1 x 100 cm column of Sephadex G-25 (superfine type; Pharmacia LKB, Uppsala, Sweden) eluted with 0.1 M acetic acid containing 0.1% BSA. Two clearly separated radioactive peaks prior to free 1251 peak were obtained.…”

Section: Methodsmentioning

confidence: 99%

“…The specific binding of '251-cVIP was measured by the use of the method of Gonzales et al (15,16) with modifications. The diluent used was TE buffer.…”

Section: Methodsmentioning

confidence: 99%

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A Vasoactive Intestinal Peptide Binding Component in Hen Granulosa Cells

Kawashima

Takahashi

Yasuoka

et al. 1995

Experimental Biology and Medicine

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In radioligand assays, the vasoactive intestinal peptide (VIP) binding component in the membrane fraction of granulosa cells of the ovary of the hen was shown to possess characteristic properties of a receptor, such as reversible binding, binding specificity, high-affinity, and limited capacity. The binding site was of a single class. The binding affinity was higher in the largest (F1) and the second largest follicle (F2) than in the third largest follicle (F3), and the binding capacity was greater in F2 and F3 than in F1. During the ovulatory cycle, changes in affinity and capacity were observed only in F1 shortly before ovulation. The results suggest the presence of VIP receptor in the hen granulosa cells and its binding is assumed to be related to the follicular growth and ovulation.

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Section: Discussionsupporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

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A Vasoactive Intestinal Peptide Binding Component in Hen Granulosa Cells

Kawashima

Takahashi

Yasuoka

et al. 1995

Experimental Biology and Medicine

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“…It has been suggested that a single VIP receptor is expressed and functions in nonmammalian species [31]. In birds, VIP receptors are present on the surface membranes of the anterior pituitary [32][33][34], the hypothalamus [35], the small intestine, and the granulosa cells [36]. VIP stimulation increases levels of intracellular cAMP in chicken pituitary cells [37] and in the hypothalamus [38].…”

Section: Introductionmentioning

confidence: 99%

Expression of Vasoactive Intestinal Peptide Receptor Messenger RNA in the Hypothalamus and Pituitary Throughout the Turkey Reproductive Cycle1

Chaiseha

Youngren

Halawani

2004

Biology of Reproduction

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Vasoactive intestinal peptide (VIP) has been implicated in the regulation of avian reproductive activity and appears to act at the level of the hypothalamus and pituitary. This in situ hybridization histochemistry study describes the distribution of VIP receptor mRNA expression in the hypothalamus and the pituitary of reproductively active (laying) and quiescent (nonphotostimulated, incubating, and photorefractory) female turkeys and characterizes the differences observed in VIP receptor gene expression. VIP receptor mRNA, while expressed throughout the hypothalamus, was specifically expressed in areas known to contain GnRH-I neurons in the chicken, i.e., the lateral septum, medial preoptic area, anterior hypothalamus, and paraventricular nucleus. Significant differences in VIP receptor mRNA expression between different reproductive states was observed only within the infundibular nuclear complex. VIP receptor mRNA was markedly less in nonphotostimulated and photorefractory hens as compared with laying and incubating hens. The most dense VIP receptor mRNA was found in the anterior pituitary, where it was 2.4- and 3.0-fold greater in laying and incubating hens, respectively, as compared with that in nonphotostimulated ones. Hens that stopped incubating and became photorefractory displayed pituitary VIP receptor mRNA levels similar to those of nonphotostimulated birds. The changes in pituitary VIP receptor mRNA expression were positively correlated with known changes in pituitary prolactin (PRL) mRNA expression and PRL content and release. These findings indicate that the variations in PRL secretion observed across the turkey reproductive cycle are, in part, regulated by changes in VIP receptors at the pituitary level.

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“…Vasoactive intestinal peptide (VIP), a brain-gut peptide, is known to stimulate the release of prolactin from the pituitary gland of the turkey hen both in vivo and in vitro (EL HALAWANI et al, 1990) and from the pituitary gland of the hen in vivo (GONZALES et al, 1994a). We have found the presence of VIP receptor in the membrane fraction of the pituitary gland of the hen (GONZALES et al, 1994a) and the amount of specific binding of the VIP receptor in the cephalic lobe of the anterior pituitary is greater in laying hens than in roosters (GONZALES et al, 1994b).…”

Section: Introductionmentioning

confidence: 99%