Prophylactic effects of caprine serum factor (CSF‐I) in mice infected with Salmonella typhimurium

Abstract

Administration of caprine serum fraction-immunomodulator (CSF-I) resulted in an enhanced survival rate in female Swiss Webster mice infected with approximately 5,000 colony-forming units of Salmonella typhimurium. The prophylactic benefit of CSF-I resulted in a significant reduction in mortality of treated mice vs. control mice. Dependent upon the inoculum's titer, 80-90% of the control group was lost due to salmonellosis by 8 days postchallenge. A single 0.1 ml (3 mg) subcutaneous dose of CSF-I given 1 day before challenge improved the survival by 70-80% and dramatically reduced the presence of S. typhimurium in splenic tissue. No prophylactic benefits were observed if CSF-I was given 4 days in advance of the pathogenic challenge. The prophylactic benefits of CSF-I developed in a dose-dependent manner. CSF-I enhances the ability of the host to withstand and survive the challenge of an infectious agent. Drug Dev. Res. 54:45-51, 2001.

Serum Derived Transfer Factor Stimulates the Innate Immune System to Improve Survival Traits in High Risk Pathogen Scenarios

“…The serum derived TF herein is a research preparation and not commercially available but is likely to be found in a USDA approved immunostimulant identified as PulmoClear TM (Colorado Serum Company, Denver, CO). Previous research demonstrated that it provided similar benefits to Salmonella ‐infected mice [Willeford et al, ]. PulmoClear is derived from caprine serum but possesses high molecular weight proteins (e.g., IgG and albumin) that could initiate a site reaction or produce delayed hypersensitivity reactions upon recurrent use.…”

Reducing Mortality in Salmonella enterica Serovar Typhimurium-Infected Mice with a Tripeptidic Serum Fraction

“…Four-week-old, female Swiss Webster mice were acclimated for 2 weeks as described previously (10). Mice comprising the control and treated populations were injected intraperitoneally with 0.1 ml of Salmonella enterica serovar Typhimurium ATCC 14028 (ϳ5.00 ϫ 10 3 bacteria per mouse) on day 0.…”

An innate immune regulatory factor (IIRF) prevents tumorogenesis in a murine melanoma challenge model

“…Female Swiss Webster mice (4 weeks of age) were purchased from Charles Rivers Laboratories (Wilmington, MA) for use in the salmonellosis study conducted on NK-depleted mice. The care and housing practices used were described previously [Willeford et al, 2001]. Female B6C3F1 mice (6 to 10 weeks of age) were obtained through the National Cancer Institute's animal program for use in the melanoma challenge study conducted at LSU.…”