Propionate Converting Anaerobic Microbial Communities Enriched from Distinct Biogeochemical Zones of Aarhus Bay, Denmark under Sulfidogenic and Methanogenic Conditions

Ozuolmez, Derya; Stams, Alfons Johannes Maria; Plugge, Caroline M.

doi:10.3390/microorganisms8030394

Cited by 11 publications

(4 citation statements)

References 60 publications

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“…Data analyses were performed using ChromQuest (Thermo Scientific, Waltham, MA, USA) and Chromeleon software (Thermo Scientific, Waltham, MA). Sulfate and sulfide were quantified as described in [24].…”

Section: Methodsmentioning

confidence: 99%

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Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark

et al. 2020

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The conventional perception that the zone of sulfate reduction and methanogenesis are separated in high- and low-sulfate-containing marine sediments has recently been changed by studies demonstrating their co-occurrence in sediments. The presence of methanogens was linked to the presence of substrates that are not used by sulfate reducers. In the current study, we hypothesized that both groups can co-exist, consuming common substrates (H2 and/or acetate) in sediments. We enriched butyrate-degrading communities in sediment slurries originating from the sulfate, sulfate–methane transition, and methane zone of Aarhus Bay, Denmark. Sulfate was added at different concentrations (0, 3, 20 mM), and the slurries were incubated at 10 °C and 25 °C. During butyrate conversion, sulfate reduction and methanogenesis occurred simultaneously. The syntrophic butyrate degrader Syntrophomonas was enriched both in sulfate-amended and in sulfate-free slurries, indicating the occurrence of syntrophic conversions at both conditions. Archaeal community analysis revealed a dominance of Methanomicrobiaceae. The acetoclastic Methanosaetaceae reached high relative abundance in the absence of sulfate, while presence of acetoclastic Methanosarcinaceae was independent of the sulfate concentration, temperature, and the initial zone of the sediment. This study shows that there is no vertical separation of sulfate reducers, syntrophs, and methanogens in the sediment and that they all participate in the conversion of butyrate.

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Section: Methodsmentioning

confidence: 99%

“…Pyrosequencing data was analyzed as described in [24]. The relative amount of reads of every operational taxonomic unit (OTU) to the total amount of reads per sample was quantified, and the average relative amount of reads per representative OTU of each slurry sample was calculated.…”

Section: Analysis and Interpretation Of The Pyrosequencing Datamentioning

confidence: 99%

Butyrate Conversion by Sulfate-Reducing and Methanogenic Communities from Anoxic Sediments of Aarhus Bay, Denmark

et al. 2020

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“…In our study, the abundance of Desulfovibrio increased and propionate metabolism decreased in the active smoking group. Sulfate reducing bacteria including the Desulfovibrio genera are able to grow on propionate ( Sedano-Nunez et al., 2018 ; Ozuolmez et al., 2020 ) and sulfate reduction then proceeds with propionate (but not butyrate) as the electron donor ( Chen et al., 2017a ; He et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Active Smoking Induces Aberrations in Digestive Tract Microbiota of Rats

Wang

et al. 2021

Front. Cell. Infect. Microbiol.

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Cigarette smoking could have certain effects on gut microbiota. Some pioneering studies have investigated effects of active smoking on the microbiome in local segments of the digestive tract, while active smoking-induced microbiome alterations in the whole digestive tract have not been fully investigated. Here, we developed a rat model of active smoking and characterized the effects of active smoking on the microbiota within multiple regions along the digestive tract. Blood glucose and some metabolic factors levels, the microbial diversity and composition, relative abundances of taxa, bacterial network correlations and predictive functional profiles were compared between the control group and active smoking group. We found that active smoking induced hyperglycemia and significant reductions in serum insulin and leptin levels. Active smoking induced region-specific shifts in microbiota structure, composition, network correlation and metabolism function along the digestive tract. Our results demonstrated that active smoking resulted in a reduced abundance of some potentially beneficial genera (i.e. Clostridium, Turicibacter) and increased abundance of potentially harmful genera (i.e. Desulfovibrio, Bilophila). Functional prediction suggested that amino acid, lipid, propanoate metabolism function could be impaired and antioxidant activity may be triggered. Active smoking may be an overlooked risk to health through its potential effects on the digestive tract microbiota, which is involved in the cause and severity of an array of chronic diseases.

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“…This Special Issue gathers seven articles on anaerobes with roles in several biogeochemical cycles. One article involves the inorganic carbon cycle [ 4 ], two describe the relationship between predominant physiological types of prokaryotes in marine sediments and propionate and butyrate degradation through sulphate reduction, fermentation, and methanogenesis in marine sediments [ 5 , 6 ], two involve the discovery of novel biodiversity involved in the sulphur cycle [ 7 , 8 ], and two focus on the role of iron in anaerobic methane oxidation [ 9 ] and in the facilitation of anaerobic long chain fatty acid degradation [ 10 ].…”

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confidence: 99%