Propionibacterium from Liver and Lung of Pigs and Guinea Pigs. 1: Physiological Identification

Oomi, H.; Azuma, Ryozo; Suzuki, Shinichi; Watanabe, Tadao; Murakami, Satoshi

doi:10.1006/anae.2002.0413

Cited by 3 publications

(2 citation statements)

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“…John [10] suspected that the infective Ascaris larvae carried the intestinal bacteria from the intestine to the liver in the ascariasis pigs with the severe hepatic inflammatory lesions. However, these intestinal bacteria were also isolated from the site of normal hepatic parenchyma without milk-spotted lesions, which is characteristic to an ascariasis [12,13,15]. Furthermore immunohistochemical detection of antigens using polyclonal antibodies against non-transformed E. coli strain K12 C600 (DakoCytomation, Inc., Tokyo, Japan) did not show a relationship between the distributions of positive antigens and hepatic white spot lesions [12].…”

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Relationship between Bacterial Translocation and Isolation of Enteric Bacteria from Extraintestinal Organs in Slaughtered Pigs

Murakami

Kanazawa

Sugishima³

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Enteric bacteria, especially Escherichia coli, have been isolated from porcine livers affected with ascariasis. We hypothesized that reason for this bacterial isolation was due to bacterial translocation (BT). In order to clarify the association between ascariasis and isolation of Enterobacteriaceae in the livers,12 pigs with ascariasis (ascariasis group), 12 pigs without ascariasis (non-ascariasis A group) were used. Jejunum, ileum, cecum, colon, livers, mesenteric lymph nodes (MLN), and portal blood were obtained from these pigs. Furthermore, in order to confirm the presence of BT in pigs, the samples mentioned above as well as spleens were obtained from 11 pigs without ascariasis (non-ascariasis B group) and 6 specific pathogen free (SPF) pigs without ascariasis (non-ascariasis SPF group). In the first experiment, the Gram-negative bacteria were isolated from livers (66.7%), MLN (91.7%), and portal blood (55.6%) regardless the presence of ascariasis. In the second experiment, isolation rates of Gram-negative and -positive bacteria were 52.9 and 66.7% for livers, 52.9 and 80% for MLN, 11.8 and 26.7% for spleens, and 40 and 20% for portal blood of the pigs examined, respectively. E. coli and Staphylococcus were the predominant isolates from these pigs. A large number of antigens immunodetected by E. coli polyclonal antibody were found in the damaged cecal mucosa. These findings present evidence that BT is generally observed in slaughtered pigs regardless the presence of ascariasis. This has challenged our notion that extraintestinal organs of pigs are usually maintained as sterile.

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“…[12], Lactobacillus casei [13], Lactobacillus salivalius [13], Propionibacterium sp. [12], Propionibacterium acnes, Propionibacterium granulosum, Propionibacterium avidum [15], Enterococcus sp. or Streptococcus sp.…”

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confidence: 99%