Propofol induces endoplasmic reticulum (ER) stress and apoptosis in lung cancer cell H460

Abstract

Propofol is one of the most commonly used intravenous anesthetic agents during cancer resection surgery. It can influence proliferation, motility, and invasiveness of cancer cells in vitro and in vivo. However, the role of the propofol in the lung cancer cells remains unclear. In this study, we demonstrated the effects of propofol on the proliferation and the apoptosis of lung cancer cell H460 by using colony formation assay and flow cytometry. Propofol also decreased tumor size and weight in established xenografted tumors. Furthermore, propofol-induced endoplasmic reticulum (ER) stress was determined by Western blot.

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells

“…Caspase-3 is a cysteine protease, which are pro-apoptotic proteins with prominent roles in ERS-induced cell death [42, 43]. ERS is well-characterized in human LC [44, 45]. Recent evidence has suggested that ERS can initiate internal pathways that may induce cellular death [27].…”

“…Calcium imbalance or aberrant protein folding in the ER result in ERS and induce cell destruction responses [47, 48]. Alterations in the ER molecular chaperone levels can suppress tumor growth in vivo [44]. Likewise, ERS-induced apoptosis is also highly dependent on the upregulation of CHOP [49].…”

Effects of propofol on colon cancer metastasis through STAT3/HOTAIR axis by activating WIF‐1 and suppressing Wnt pathway

“…Previous study revealed that propofol could inhibit cancer growth and invasion through Wnt/β‐catenin pathway . Through inducing endoplasmic reticulum stress, propofol increased cell apoptosis in lung cancer . Moreover, propofol could also suppress the pancreatic cancer proliferation and invasion via microRNA‐133a .…”

“…11 Through inducing endoplasmic reticulum stress, propofol increased cell apoptosis in lung cancer. 13 Moreover, propofol could also suppress the pancreatic cancer proliferation and invasion via microRNA-133a. 14 Besides, propofol has also been reported to inhibit the colon cancer invasion, via downregulation of MMP-2 and MMP-9.…”

“…10 Besides, recent studies even found that propofol also exerts antitumor activities in several types of cancers, such as breast cancer, lung cancer, pancreatic cancer, hepatocellular carcinoma, and colon cancer. [11][12][13][14][15] Although some of the results remain controversial, overall, propofol has been considered to possesses an antitumor role on the proliferation, invasion, and metastasis of tumor cells via regulating key RNAs and signaling pathways. 10 However, the underlying mechanisms about how propofol suppresses the development of colon cancer remain unclear.…”