Proportionality of ELF electric field-induced growth inhibition to induced membrane potential inZea mays andVicia faba roots

Brayman, Andrew A.; Megumi, Tsuneo; Miller, Morton W.

doi:10.1007/bf01210558

Cited by 12 publications

(3 citation statements)

References 16 publications

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“…The biophysical basis of the initial stages of sensory transduction depend greatly on the nature of the stimulus, but a subthreshold change in membrane potential resulting from a change in mean channel conductance mediates all forms of sensory transduction [10]. The effects of EMFs on membrane potential have been studied [11][12][13][14], but EMF-induced changes of less than about 1000 mV could not be detected because the methods of measurement were relatively insensitive. We developed a sensitive measurement method and studied the effect of magnetic fields on the cell resting membrane potential as a means of testing the sensory-transduction hypothesis.…”

Section: Research Articlementioning

confidence: 99%

Resting potential of excitable neuroblastoma cells in weak magnetic fields

Sonnier

Kolomytkin

Marino

2000

CMLS, Cell. Mol. Life Sci.

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The mechanism by which static and low-frequency magnetic fields are transduced into biological signals responsible for reported effects on brain electrical activity is not yet ascertained. To test the hypothesis that fields can cause a subthreshold change in the resting membrane potential of excitable cells, we measured changes in transmembrane current under voltage clamp produced in SH-SY5Y neuroblastoma cells, using the patch-clamp method in the whole-cell configuration. In separate experiments, cells were exposed to static fields of 1, 5, and 75 G, to time-varying fields of 1 and 5 G, and to combined static and time-varying fields tuned for resonance of Na+, K+, Ca2+, or H+. To increase sensitivity, measurements were made on cells connected by gap junctions. For each cell, the effect of the field was evaluated on the basis of 100 trials consisting of a 5-s exposure immediately followed by a 5-s control period. In each experiment, the field had no discernible effect on the transmembrane current in the vicinity of zero current (- 50 mV voltage clamp). The sensitivity of the measuring system was such that we would have detected a current corresponding to a change in membrane potential as small as 38 microV. Consequently, if sensitivity of mammalian cells to magnetic fields is mediated by subthreshold changes in membrane potential, as in sensory transduction of sound, light, and other stimuli, then the ion channels responsible for the putative changes are probably present only in specialized sensory neurons or neuroepithelial cells. A change in transmembrane potential in response to magnetic fields is not a general property of excitable cells in culture.

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Section: Research Articlementioning

confidence: 99%

Resting potential of excitable neuroblastoma cells in weak magnetic fields

Sonnier

Kolomytkin

Marino

2000

CMLS, Cell. Mol. Life Sci.

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“…Simulating internal signals, the electromagnetic radiation activates the available reserves and accelerates the adaptation and recovery processes aimed at eliminating damages [16].…”

Section: Research Results and Discussionmentioning

confidence: 99%

Study of the effects of ultra-low intensity electromagnetic fields on biological objects

Voloshyn

Kulish

Oliinyk

et al. 2021

TAPR

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The object of research is the efficiency of exposure to electromagnetic field (EMF) of ultra-low intensity on biological objects, which is formed by a generator of broadband radiation. The principle of action of the generator is based on formation of electromagnetic radiation induced by periodic pulsed gas discharge in coaxial system of electrodes, which is loaded on a dielectric rod antenna. The method of selection of signals and corresponding equipment, which energy characteristics of radiation correspond to the criterion of non-thermal influence on bioobjects, is developed for obtaining a comparative assessment of influence bioefficiency. The proposed new method for processing experimental data using statistical calculations that meet the requirements for the processing and interpretation of the results. The seeds of wheat and interaction of millimeter range electromagnetic oscillations with bone marrow cells of rats were used as biological objects for investigating the effect of millimeter range electromagnetic oscillations. A biosensory effect was obtained when exposed to broadband radiation of ultra-low intensity, compared to the control sample. A change in the properties of the seeds, in particular, heat resistance, is observed. According to the experimental data, seeds turn out to be less susceptible to heat as a result of their pretreatment with EMF. The biological response is observed to depend on the frequency and time of irradiation. Also, the dependence of the decrease in the number of dead cells on the time of EMF irradiation was experimentally proved. The equation of dependence of selective average proportion of dead cells in rat bone marrow on irradiation time was calculated. Biosensory effect of exposure to broadband ultra-low intensity EMF of the developed emitter was revealed. It was established and statistically proved that the minimum time with the maximum positive effect of exposure to electromagnetic radiation of millimeter range on bone marrow cells of rats is 30 minutes, compared with an unirradiated sample. The results make it possible to evaluate the positive effect of electromagnetic oscillations on biological objects and propose the results of studies for practical use in the development of medical systems.

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“…The V mi associated with the apparent threshold for growth inhibition was similar in Zea mais and Vicia faba roots (2.5 vs. 2.4 mV, respectively). At V mi above this threshold, Zea mais root growth rate declined by about 9% per mV and Vicia faba root growth declined by about 19% per mV induced potential [Brayman et al, 1990].…”

Section: Introductionmentioning

confidence: 99%

Modification of electrokinetic properties of nuclei in human buccal epithelial cells by electric fields

Shckorbatov

Shakhbazov

Rudenko

2001

Bioelectromagnetics

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The influence of an alternating (50 Hz) electric field (5--110 V/cm) on the state of human buccal epithelium cells was studied by the methods of intracellular microelectrophoresis, heterochromatin staining with orcein, and indigo carmine staining for viability and membrane integrity evaluations. Electric field exposure induced an increase in electrophoretic mobility of cell nuclei, decreased numbers of heterochromatin granules near the inner membrane of cell nucleus, and induced cell membrane damage; but cell viability was conserved. Nuclear and cell membrane properties varied with electric field strength and age of the donors. The data obtained are interpreted as evidence of electric field induced activation of the functional state of nuclei.

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Proportionality of ELF electric field-induced growth inhibition to induced membrane potential inZea mays andVicia faba roots

Cited by 12 publications

References 16 publications

Resting potential of excitable neuroblastoma cells in weak magnetic fields

Resting potential of excitable neuroblastoma cells in weak magnetic fields

Study of the effects of ultra-low intensity electromagnetic fields on biological objects

Modification of electrokinetic properties of nuclei in human buccal epithelial cells by electric fields

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