Propyl gallate and butylated hydroxytoluene influence the accumulation of saturated fatty acids, omega-3 fatty acid and carotenoids in thraustochytrids

Singh, Deepti; Mathur, Anshu S.; Tuli, Deepak K.; Puri, Munish; Barrow, Colin J.

doi:10.1016/j.jff.2015.03.022

Cited by 25 publications

(12 citation statements)

References 27 publications

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“…The presence of salt in seawater cultured C. vulgaris (NIOT-74) biomass can interfere with the carotenoid yield as was noticed in the lower carotenoid yield from biomass without any wash. Conversely, the biomass subjected to 0.5 % HCl treatment showed the maximum carotenoid yield substantiating the efficiency of dilute acids to remove the salts adhered to cell wall and facilitate optimal solvent penetration [ 39 ]. Similar to the present study, Singh et al [ 41 ], have also demonstrated the efficiency of dilute (0.5 %) HCl treatment for enhanced carotenoid yield from Thraustochytrium sp. [ 41 ].…”

Section: Resultssupporting

confidence: 89%

“…Similar to the present study, Singh et al [ 41 ], have also demonstrated the efficiency of dilute (0.5 %) HCl treatment for enhanced carotenoid yield from Thraustochytrium sp. [ 41 ]. Nevertheless, higher acid concentration (6 N) resulted in lower carotenoid yield, this might have been due to degradation of carotenoids reported at higher acid concentration [ 43 ].…”

Section: Resultssupporting

confidence: 89%

“…Nonetheless, when the acid concentration was increased from 2% to 6 N the lutein content declined drastically to 0.35 mg/g. This lower lutein content noticed at high acid concentrations could be attributed to the degradation effect brought forth by strong acids as was evidenced for another xanthophyll carotenoid astaxanthin by Singh et al, [ 41 ]. Furthermore, the biomass treated with 0.5 M ammonium bicarbonate gave a moderately high lutein content (8.33 ± 0.54 mg/g).…”

Section: Resultsmentioning

confidence: 70%

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A critical look into different salt removal treatments for the production of high value pigments and fatty acids from marine microalgae Chlorella vulgaris (NIOT-74)

Leema

Jothy

Peter

et al. 2021

Biotechnology Reports

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Highlights Seawater cultivation of marine microalgae for nutraceutical production is hampered by the presence of salt. Dilute hydrochloric acid (0.5 %) pre-wash developed as effective salt removal approach to augment total carotenoid and lutein content (1.8 fold). Salt removal treatments also impacted the fatty acid profile and degree of saturation. Impact of salt removal treatment was visualized using SEM.

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 70%

See 1 more Smart Citation

A critical look into different salt removal treatments for the production of high value pigments and fatty acids from marine microalgae Chlorella vulgaris (NIOT-74)

Leema

Jothy

Peter

et al. 2021

Biotechnology Reports

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“…If the omega-3 fatty acids could be separated from other fatty acids in the Thraustochytrium sp. AMCQS5-5 extract, then these fatty acids, particularly DHA (docosahexaenoic acid), could be used as nutritional products and offset the cost of biofuel production in this strain [ 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

Comparison of Cell Disruption Methods for Improving Lipid Extraction from Thraustochytrid Strains

et al. 2015

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Lipid extraction is an integral part of biodiesel production, as it facilitates the release of fatty acids from algal cells. To utilise thraustochytrids as a potential source for lipid production. We evaluated the extraction efficiency of various solvents and solvent combinations for lipid extraction from Schizochytrium sp. S31 and Thraustochytrium sp. AMCQS5-5. The maximum lipid extraction yield was 22% using a chloroform:methanol ratio of 2:1. We compared various cell disruption methods to improve lipid extraction yields, including grinding with liquid nitrogen, bead vortexing, osmotic shock, water bath, sonication and shake mill. The highest lipid extraction yields were obtained using osmotic shock and 48.7% from Schizochytrium sp. S31 and 29.1% from Thraustochytrium sp. AMCQS5-5. Saturated and monounsaturated fatty acid contents were more than 60% in Schizochytrium sp. S31 which suggests their suitability for biodiesel production.

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“…was increased from 26.5 to 38.3 g/L, which accompanied with the lower ROS levels and higher antioxidant capacity [ 17 ]. Supplementing butylated hydroxytoluene or propyl into the media led to the increase of lipid and biomass levels in the thraustochytrids, with propyl gallate being the more effective of the two antioxidants [ 76 ]. Therefore, the combination of antioxidants and stress-based strategies appears to be a practical cultivation mode to improve the growth of microalgae.…”

Section: Manipulation Of Stresses By Different Cultivation Modesmentioning

confidence: 99%

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Sun

Ren

Zhao

et al. 2018

Biotechnol Biofuels

347

129

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Microalgae have drawn great attention as promising sustainable source of lipids and carotenoids. Their lipid and carotenoids accumulation machinery can be trigged by the stress conditions such as nutrient limitation or exposure to the damaging physical factors. However, stressful conditions often adversely affect microalgal growth and cause oxidative damage to the cells, which can eventually reduce the yield of the desired products. To overcome these limitations, two-stage cultivation strategies and supplementation of growth-promoting agents have traditionally been utilized, but developing new highly adapted strains is theoretically the simplest strategy. In addition to genetic engineering, adaptive laboratory evolution (ALE) is frequently used to develop beneficial phenotypes in industrial microorganisms during long-term selection under specific stress conditions. In recent years, many studies have gradually introduced ALE as a powerful tool to improve the biological properties of microalgae, especially for improving the production of lipid and carotenoids. In this review, strategies for the manipulation of stress in microalgal lipids and carotenoids production are summarized and discussed. Furthermore, this review summarizes the overall state of ALE technology, including available selection pressures, methods, and their applications in microalgae for the improved production of lipids and carotenoids.

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Propyl gallate and butylated hydroxytoluene influence the accumulation of saturated fatty acids, omega-3 fatty acid and carotenoids in thraustochytrids

Cited by 25 publications

References 27 publications

A critical look into different salt removal treatments for the production of high value pigments and fatty acids from marine microalgae Chlorella vulgaris (NIOT-74)

A critical look into different salt removal treatments for the production of high value pigments and fatty acids from marine microalgae Chlorella vulgaris (NIOT-74)

Comparison of Cell Disruption Methods for Improving Lipid Extraction from Thraustochytrid Strains

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

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