Prospective comparison of two methods of screening for diabetic retinopathy by nonmydriatic fundus camera

Romero-Aroca, Pedro; Sagarra-Alamo, Ramon; Basora-Gallisa, Josep; Basora-Gallisa, Teresa; Baget-Bernaldiz, Marc; Bautista-Pérez, Ángel

doi:10.2147/opth.s14521

Cited by 29 publications

(15 citation statements)

References 18 publications

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“…Diabetic retinopathy is the most common microvascular complication of diabetes (Antonetti et al, 2012), and after two decades of diabetes, almost all T1D patients will have some degree of retinopathy, as will more than 80% of insulin-treated T2D patients and 50% of those not requiring insulin (Klein et al, 1989;Romero-Aroca et al, 2010).…”

Section: The Global Significance Of Diabetic Retinopathymentioning

confidence: 99%

The progress in understanding and treatment of diabetic retinopathy

Stitt

Curtis

Chen

et al. 2016

Progress in Retinal and Eye Research

875

657

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Section: The Global Significance Of Diabetic Retinopathymentioning

confidence: 99%

The progress in understanding and treatment of diabetic retinopathy

Stitt

Curtis

Chen

et al. 2016

Progress in Retinal and Eye Research

875

657

View full text Add to dashboard Cite

“…At present, the number of patients with diabetes has reached 90 million in China 2 . After 20 years of diabetes, nearly all patients with type I diabetes, 80% of patients with type 2 diabetes requiring insulin treatment and 50% of those not requiring insulin treatment exhibit some degree of DR 3 .…”

mentioning

confidence: 99%

DJ-1 protects retinal pericytes against high glucose-induced oxidative stress through the Nrf2 signaling pathway

Wang

Zhao

Chen

2020

Sci Rep

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Oxidative stress has been associated with the etipathogenesis of Diabetic retinopathy (DR). Studies have shown that DJ-1 plays an important role in regulating the reactive oxygen species (ROS) production and resistance to oxidative stress-induced apoptosis. This study aimed to investigate whether DJ-1 upregulates oxidative stress and prevents damage to retinal capillary pericytes by increasing antioxidant capacity through the Nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway. Nrf2 is a redox-sensitive transcription factor that encode antioxidant enzymes and phase II metabolic enzymes, activation of Nrf2 functions is one of the critical defensive mechanisms against oxidative stress in many tissues. Our results showed after DJ-1 overexpression, apoptosis of rat retinal pericytes (RRPs) decreased, the ratio of B-cell lymphoma-2 (Bcl-2) to BCL2-Associated X Protein (BAX) increased, the production of ROS decreased, and the protein expression and activity of manganese superoxide dismutase (MnSOD, also called SOD2) and catalase (CAT) increased. DJ-1 overexpression activated Nrf2 expression, however, after Nrf2 silencing, apoptosis of RRPs increased, the ratio of Bcl-2 to BAX decreased, the production of ROS increased, the protein expression of MnSOD and CAT decreased, and the expression of heme oxygenase-1 (HO-1), NADP(H) quinone oxidoreductase (NQO1), glutamate-cysteine ligase catalytic subunit (GCLC) and modifier subunit (GCLM) decreased. These data suggest that enhancement of the Nrf2 pathway is a potential protective strategy for the treatment of DR. Therefore, DJ-1 may prevent high glucose-induced oxidative stress and RRPs apoptosis through the Nrf2 signaling pathway, thereby preventing the early onset and progression of DR. Quantification of ROS. Cellular ROS production was evaluated by measuring the oxidative conversion of cell-permeable dichlorodihydrofluorescein diacetate (DCFH-DA; Beyotime Institute of Biotechnology) to green fluorescent 2' ,7'-dichlorofluorescein (DCF;). DCFH-DA was dissolved in dimethyl sulfoxide to a final concentration of 5 μM. RRPs (1 × 10 6 cells/ml) were incubated with 2 mM DCFH-DA for 20 min under standard conditions, washed three times in PBS and resuspended. The RRPs were assessed with a flow cytometer (FACScan; BD Biosciences) equipped with FACSComp software (Quadra 650) at excitation/emission wavelengths of 488/525 nm.

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“…Currently, there is a lack of evidence on screening specifically for DME; however, detection of DME is important because this condition is the leading cause of blindness in patients with type 2 diabetes [97]. One study from a DME screening program in Hong Kong demonstrated a high false positive rate (86.6%) and a low positive predictive value (13.4%) for screening of DME with mydriatic FP, if macular thickness was used to define the presence of macular edema [98].…”

Section: Screening For Dmementioning

confidence: 99%

Fundamental principles of an effective diabetic retinopathy screening program

et al. 2020

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Background Diabetic retinopathy (DR) is the leading cause of blindness among working-age adults worldwide. Early detection and treatment are necessary to forestall vision loss from DR. Methods A working group of ophthalmic and diabetes experts was established to develop a consensus on the key principles of an effective DR screening program. Recommendations are based on analysis of a structured literature review. Results The recommendations for implementing an effective DR screening program are: (1) Examination methods must be suitable for the screening region, and DR classification/grading systems must be systematic and uniformly applied. Twofield retinal imaging is sufficient for DR screening and is preferable to seven-field imaging, and referable DR should be well defined and reliably identifiable by qualified screening staff; (2) in many countries/regions, screening can and should take place outside the ophthalmology clinic; (3) screening staff should be accredited and show evidence of ongoing training; (4) screening programs should adhere to relevant national quality assurance standards; (5) studies that use uniform definitions of risk to determine optimum risk-based screening intervals are required; (6) technology infrastructure should be in place to ensure that high-quality images can be stored securely to protect patient information; (7) although screening for diabetic macular edema (DME) in conjunction with DR evaluations may have merit, there is currently insufficient evidence to support implementation of programs solely for DME screening. Conclusion Use of these recommendations may yield more effective DR screening programs that reduce the risk of vision loss worldwide.

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Prospective comparison of two methods of screening for diabetic retinopathy by nonmydriatic fundus camera

Cited by 29 publications

References 18 publications

The progress in understanding and treatment of diabetic retinopathy

The progress in understanding and treatment of diabetic retinopathy

DJ-1 protects retinal pericytes against high glucose-induced oxidative stress through the Nrf2 signaling pathway

Fundamental principles of an effective diabetic retinopathy screening program

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