Prostaglandin biosynthesis by rabbit renomedullary interstitial cells in tissue culture. Stimulation by angiotensin II, bradykinin, and arginine vasopressin.

Zusman, Randall M.; Keiser, Harry R.

doi:10.1172/jci108758

Cited by 335 publications

(125 citation statements)

References 43 publications

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“…In the medulla prostaglandins are also formed in the interstitial cells (Bohman, 1977;Zusman & Keiser, 1977) and two prostaglandin synthetases with differing pH optima have been isolated from medullary homogenates (Raz & Schwartzman, 1976). In the renal cortex prostaglandin synthesis is probably confined to arteriolar endothelial cells, although staining to cyclo-oxygenase antibodies has also been shown in the epithelial cells of Bowman's capsule in the rabbit and glomerular mesangial cells in the cow.…”

Section: Discussionmentioning

confidence: 99%

Prostaglandin synthesis and renal function in man.

Haylor¹

1980

The Journal of Physiology

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SUMMARY1. Experiments were performed to determine the changes in renal function which occur following prostaglandin synthetase inhibition in healthy conscious humans. It was hoped that such experiments could provide information on the mechanism by which renal prostaglandin synthesis influences urinary excretion.2. In water-diuretic male subjects (receiving a slow saline infusion) the renal excretion of sodium and water was reduced following i.v. acetylsalicylic acid (1 g) administration, while the effective renal plasma flow (p-aminohippurate clearance), and glomerular filtration rate (inulin clearance) remained unaltered. 3. In normally hydrated female subjects on an unrestricted diet, the mean urinary prostaglandin E output was 8-5 ng/hr. The renal excretion of sodium, water and urinary prostaglandin E were significantly reduced (P < 0.05) following oral acetylsalicylic acid (1 -2 g) administration.4. In normally hydrated female subjects on an unrestricted diet the renal excretion of sodium and water was reduced following oral paracetamol (1-5 g) administration.5. It is concluded that following renal prostaglandin synthetase inhibition in conscious humans, the excretion of sodium and water can be reduced without measurable changes in the glomerular filtration rate or effective renal plasma flow. It is suggested that in conscious healthy humans, the kidney may continually synthesize prostaglandin which might help to maintain sodium and water excretion by a direct action on the renal tubule without influencing renal blood flow. The relevance of this hypothesis to the intrarenal location of prostaglandin synthetase is discussed.

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Section: Discussionmentioning

confidence: 99%

Prostaglandin synthesis and renal function in man.

Haylor¹

1980

The Journal of Physiology

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“…It has been observed that perfusion of the renal medulla is sensitive to COX inhibition (110,113,119). Furthermore, renomedullary interstitial cells have receptors for ANG II and BK and release PGE 2 in response to these agents (21, 72,178,179,186). Recently, Qi et al (127) have shown that the COX-2 isoform is expressed in those cells and is responsible for this action.…”

Section: Medullary Po2 and Perfusion Of The Medullamentioning

confidence: 99%

Physiology of the renal medullary microcirculation

Pallone

Zhang

Rhinehart

2003

American Journal of Physiology-Renal Physiology

187

193

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Pallone, Thomas L., Zhong Zhang, and Kristie Rhinehart. Physiology of the renal medullary microcirculation. Am J Physiol Renal Physiol 284: F253-F266, 2003; 10.1152/ajprenal.00304.2002.-Perfusion of the renal medulla plays an important role in salt and water balance. Pericytes are smooth muscle-like cells that impart contractile function to descending vasa recta (DVR), the arteriolar segments that supply the medulla with blood flow. DVR contraction by ANG II is mediated by depolarization resulting from an increase in plasma membrane Cl Ϫ conductance that secondarily gates voltage-activated Ca 2ϩ entry. In this respect, DVR may differ from other parts of the efferent microcirculation of the kidney. Elevation of extracellular K ϩ constricts DVR to a lesser degree than ANG II or endothelin-1, implying that other events, in addition to membrane depolarization, are needed to maximize vasoconstriction. DVR endothelial cytoplasmic Ca 2ϩ is increased by bradykinin, a response that is inhibited by ANG II. ANG II inhibition of endothelial Ca 2ϩ signaling might serve to regulate the site of origin of vasodilatory paracrine agents generated in the vicinity of outer medullary vascular bundles. In the hydropenic kidney, DVR plasma equilibrates with the interstitium both by diffusion and through water efflux across aquaporin-1. That process is predicted to optimize urinary concentration by lowering blood flow to the inner medulla. To optimize urea trapping, DVR endothelia express the UT-B facilitated urea transporter. These and other features show that vasa recta have physiological mechanisms specific to their role in the renal medulla. vasa recta; perfusion; hypertension; oxygenation; urinary concentration; patch clamp; calcium; fura 2 THE MICROCIRCULATION OF THE kidney is regionally specialized. In the cortex, afferent and efferent arterioles govern the driving forces that promote glomerular filtration. A dense peritubular capillary plexus arising from efferent arterioles surrounds the proximal and distal convoluted tubules to accommodate enormous reabsorption of glomerular filtrate. In contrast, vasa recta serve needs specific to the medulla. Through the counterflow arrangement of descending (DVR) and ascending vasa recta (AVR), countercurrent exchange traps NaCl and urea deposited to the interstitium by collecting ducts and the loops of Henle. This is vital to maintain corticomedullary osmotic gradients but conflicts with the need to supply nutrient blood flow to medullary tissue. Metabolic substrates that enter the medulla in DVR blood diffuse to the AVR to be shunted back to the cortex. To deal with the threat of medullary hypoxia resulting from this process, the kidney has evolved a capacity to exert subtle control over regional perfusion of the outer and inner medulla. The details are far from clear, but much experimental evidence points to the complex interactions of many autocoids and paracrine agents to modulate vasomotor tone at various sites along the microvascular circuit. The goal of this review is to summarize ...

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“…Both AVP and ANG II stimulate renal synthesis of prostaglandins (PG) in vivo and in vitro (7)(8)(9). Rat glomerular epithelial and mesangial cells in culture pro- ' Abbreviations used in this paper: ANG II, angiotensin II; AVP, arginine vasopressin; d(CH2)5VDAVP, [1-(,-mer-capto-#0-cyclopentamethylene propionic acid)-4-valine, 8-D-arginine] vasopressin; dDAVP, 1-desamino-8-D-arginine vasopressin; DMSO, dimethyl sulfoxide; GFR, glomerular filtration rate; Kf, ultrafiltration coefficient; 6kPGFI., 6-keto-prostaglandin Fla; PG, prostaglandin(s), with the corresponding letter (E, F) for each series; SNGFR, single nephron GFR; TXB2, thromboxane B2.…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin synthesis by rat glomerular mesangial cells in culture. Effects of angiotensin II and arginine vasopressin.

Scharschmidt¹,

Dünn²

1983

J. Clin. Invest.

215

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A B S T R A C T Arginine vasopressin (AVP) and angiotensin II (ANG II) reduce the glomerular filtration rate and ultrafiltration coefficient. Vasodilatory prostaglandins (PG) antagonize these effects. AVP and ANG II also cause mesangial cell contraction. Therefore, possible PG stimulation by these peptides and two vasopressin analogues was studied in cultured rat glomerular mesangial cells. The effect of altered calcium availability on PG production was also studied.Glomeruli from 75-100-g Sprague-Dawley rats were cultured in supplemented nutrient media for 28 d and experiments were performed on the first passage. Mesangial cell morphology was confirmed by electron microscopy. Cells produced PGE2 > PGF2a > 6-ketoPGFia > thromboxane B2 when incubated with the divalent cation ionophore, A23187, or arachidonic acid (C20:4). ANG II and AVP selectively stimulated PGE2 at threshold concentrations of 10 nM ANG II and 100 pM of AVP. The effects of the antidiuretic analogue 1-desamino-8-D-arginine vasopressin (dDAVP) and the antipressor analogue [1-(,-

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Prostaglandin biosynthesis by rabbit renomedullary interstitial cells in tissue culture. Stimulation by angiotensin II, bradykinin, and arginine vasopressin.

Cited by 335 publications

References 43 publications

Prostaglandin synthesis and renal function in man.

Prostaglandin synthesis and renal function in man.

Physiology of the renal medullary microcirculation

Prostaglandin synthesis by rat glomerular mesangial cells in culture. Effects of angiotensin II and arginine vasopressin.

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