Protected fat-protein response in the diet to fatty acid profile of rumen fluid

Abstract

Fat supplementation in the ration has a negative impact on rumen microbes and the biohydrogenation of fats. Meanwhile, the effectiveness of post-rumen nutrients may be decreased if high-protein diet is excessively broken down in the rumen. The protection of both fat and protein may help address fat-related issues in the rumen and enhance protein absorption in the intestine. The goal of this study was to examine the fatty acid composition of rumen fluid made with a blend of fat, protein, and Agromix® mineral premix that had been formaldehyde-protected. Two treatments were compared using an independent sample T-test (R1/control = basal diet, and R2 = basal diet with 4.4% DM replacement of protected fat-protein). All treatments were subjected to a 48-hour incubation using Tilley and Terry’s in vitro technique. The results indicated that the levels of SFA in R2 treatment showed a significant decrease (p<0.05), while the concentrations of MUFA and PUFA in R2 exhibited a more significant increase (p<0.05) compared to those in R1. It can be concluded that protected fat substitution can reduce biohydrogenation of fat in the rumen, thereby helping elevate the levels of absorbable unsaturated fatty acid levels in the intestine.