Protecting HaCaT cells from ionizing radiation using persimmon tannin-Aloe gel composite

Abstract

Context: Persimmon tannin (extract of Diospyros kaki L.f [Ebenaceae]) and Aloe gel (extract of Aloe vera (L.) Burm.f. [Asphodelaceae]) are known as anti-radiation agents. However, radiation resistance of the persimmon tannin-Aloe gel composite remains inconclusive. Objective: To investigate the capacity of the persimmon tannin-Aloe gel composite to protect against ionising radiation at the cellular level. Materials and methods: HaCaT (human epidermal keratinocytes) cells were pre-treated with PT-A-1 (the mass ratio of persimmon tannin and Aloe gel was 2:1) or the single component (persimmon tannin or Aloe gel) at various concentrations (0, 50, 100, 200, 400, 800 lg/mL. Control group: medium with no HaCaT cells), and then radiated with X-rays (radiation dose: 4, 8, 12, 16, and 20 Gy). Cell viability, cell apoptosis, and radiation-induced intracellular reactive oxygen species (ROS) generation were analysed by CCK-8, Hoechst 33258 staining/flow cytometry, and 2 0 ,7 0 -dichlorfluorescein diacetate (DCFH-DA) assay, respectively, for 12 or 24 h incubation after radiation. Results: The optimal radiation dose and post-radiation incubation period were determined to be 8 Gy and 12 h. CCK-8 activity detection showed that the cell activity was 77.85% (p < 0.05, IC 50 ¼ 55.67 lg/mL). The apoptotic rate was the lowest (4.32%) at 200 lg/mL of PT-A-1 towards HaCaT cells. ROS production was the most effectively suppressed by 200 lg/mL PT-A-1 towards HaCaT cells. Discussion and conclusions: The persimmon tannin-Aloe gel composite has good radioprotective effect, and which will facilitate its clinic application as a potential natural anti-radiation agent in future.

Activation of NRF2 by topical apocarotenoid treatment mitigates radiation-induced dermatitis

“…Next, the effects of NRF2 status on radiation-induced dermatitis in vitro were assessed using an siRNA-based knockdown approach and subsequent measurement of sensitization of skin keratinocytes (HaCaT cells) to IR-induced cell death. Numerous prior studies have used HaCaT skin keratinocytes as a valid cellular model to study cutaneous effects of ionizing radiation [ [42] , [43] , [44] , [45] , [46] , [47] ]. Specifically, at 24 h post radiation, cell confluence decreased approximately 20% in NRF2 knockdown HaCaT cells ( Fig.…”

WITHDRAWN: Effects of Persimmon Tannin-Aloe Vera Composite on Cytotoxic Activities, and Radioprotection Against X-rays Irradiated in HepG2 and L02 Cells

“…Cell culture was synthesized according to the glutaraldehyde crosslinking method described by our previous work with minor modi cations Frozen L02 and HepG2 cells were quickly thawed in warm water bath at 37°C, collected by centrifugation, and resuspended in fresh medium [16,17,18]. The thawed culture solution was aspirated (dropped into the thawed culture solution) into a 15 mL tube; the prepared fetal bovine serum content was 10%.…”

Topically applied fullerenols protect against radiation dermatitis by scavenging reactive oxygen species

“…Irradiation also caused excess intracellular ROS production in HaCaT cells and activated cleaved Caspase3, eventually leading to apoptosis [ 13 ]. Qian et al proved that 8 Gy of X-ray irradiation significantly increased ROS in HaCaT cells, eventually leading to apoptosis [ 46 ]. Lagadu et al found that 6 Gy of irradiation significantly increased the 8-OHdG levels in HFF-1 cells, leading to DNA damage [ 47 ].…”