Protection against tuberculosis by Bacillus Calmette-Guérin (BCG) vaccination: A historical perspective

Setiabudiawan, Todia Pediatama; Reurink, Remi K.; Hill, Philip C.; Netea, Mihai G.; Crevel, Reinout van; Koeken, Valerie A C M

doi:10.1016/j.medj.2021.11.006

Cited by 12 publications

(5 citation statements)

References 116 publications

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“…The observation that metabolites show less association with IFN-γ production in response to M. tuberculosis is also interesting for tuberculosis vaccine development, as this suggests that the metabolome might not be a major factor influencing the variable protection provided by BCG against tuberculosis. However, our lack of understanding of correlates of protection in the context of vaccination against TB makes it difficult to evaluate these results in the context of tuberculosis vaccination [30].…”

Section: Plos Biologymentioning

confidence: 99%

Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination

Koeken

Mourits

et al. 2022

PLoS Biol

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The antituberculosis vaccine Bacillus Calmette–Guérin (BCG) induces nonspecific protection against heterologous infections, at least partly through induction of innate immune memory (trained immunity). The amplitude of the response to BCG is variable, but the factors that influence this response are poorly understood. Metabolites, either released by cells or absorbed from the gut, are known to influence immune responses, but whether they impact BCG responses is not known. We vaccinated 325 healthy individuals with BCG, and collected blood before, 2 weeks and 3 months after vaccination, to assess the influence of circulating metabolites on the immune responses induced by BCG. Circulating metabolite concentrations after BCG vaccination were found to have a more pronounced impact on trained immunity responses, such as the increase in IL-1β and TNF-α production upon Staphylococcus aureus stimulation, than on specific adaptive immune memory, assessed as IFN-γ production in response to Mycobacterium tuberculosis. Circulating metabolites at baseline were able to predict trained immunity responses at 3 months after vaccination and enrichment analysis based on the metabolites positively associated with trained immunity revealed enrichment of the tricarboxylic acid (TCA) cycle and glutamine metabolism, both of which were previously found to be important for trained immunity. Several new metabolic pathways that influence trained immunity were identified, among which taurine metabolism associated with BCG-induced trained immunity, a finding validated in functional experiments. In conclusion, circulating metabolites are important factors influencing BCG-induced trained immunity in humans. Modulation of metabolic pathways may be a novel strategy to improve vaccine and trained immunity responses.

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Section: Plos Biologymentioning

confidence: 99%

Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination

Koeken

Mourits

et al. 2022

PLoS Biol

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“…The only preventive control of TB has relied on the BCG vaccine for more than 100 years since its first administration to humans in 1921 [1] . Previous studies have reported that BCG could protect children from severe TB and miliary TB [2] .…”

Section: Introductionmentioning

confidence: 99%

Importance of adjuvant selection in tuberculosis vaccine development: Exploring basic mechanisms and clinical implications

Gyu Choi,

Woong Kwon,

Jae Shin

2023

Vaccine: X

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“…Developing countries account for over 80% of tuberculosis (TB) cases [1]. The existing Bacille Calmette-Guerin (BCG) vaccine offers protection against severe progressive tuberculosis in children, but its efficacy in preventing the predominant adolescent form of tuberculosis, particularly pulmonary tuberculosis, which is the contagious variant of the disease, is inconsistent [2]. In addition, the co-occurrence of tuberculosis and IOP Publishing doi:10.1088/1755-1315/1271/1/012091 2 HIV/AIDS, together with the growing prevalence of multi-drug resistance (MDR) strains, significantly exacerbates the issue and impedes existing control measures.…”

Section: Introductionmentioning

confidence: 99%

The cloning of ESAT-6, EspC and Rv2029c genes obtained from an Indonesian tuberculosis-infected patient

Hidayah,

Pambudi,

Widayanti

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Early secreted antigenic target of 6-kDa protein (ESAT-6), a protein related to tuberculosis virulence (EspC) and latent antigen Rv2029c are important biomarkers for Mycobacterium tuberculosis prevention. Recent advances in subunit protein vaccines offer promising solutions to the inadequacies of existing BCG vaccines. In this study, these antigens were cloned into pET plasmid series to express the genes using the Escherichia coli expression system. Polymerase Chain Reaction was used to amplify the target genes from genome DNA isolated from Indonesia tuberculosis-infected patient’s sputum. A 309 bp of ESAT-6 fragment and a 337 bp of EspC fragment were inserted into pET-21b. Meanwhile, the 1038 bp of Rv2029c fragment was inserted into pET-32b. All constructs were transformed into Escherichia coli TOP10 and screened on Luria Bertani agar media containing ampicillin. The transformants were subjected to colony PCR using T7 universal primers followed by plasmid isolation to confirm the recombination. All genes were successfully cloned into the pET plasmid series. These results serve as a basis for further studies to express the recombinant protein as a booster vaccine.

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Protection against tuberculosis by Bacillus Calmette-Guérin (BCG) vaccination: A historical perspective

Cited by 12 publications

References 116 publications

Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination

Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination

Importance of adjuvant selection in tuberculosis vaccine development: Exploring basic mechanisms and clinical implications

The cloning of ESAT-6, EspC and Rv2029c genes obtained from an Indonesian tuberculosis-infected patient

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