Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes

Yang, Hsin Ling; Chen, Ssu Ching; Chang, Nai Wen; Chang, Jia‐Wei; Lee, Mei Ling; Tsai, Pei Chuan; Fu, Han; Kao, Wei Wan; Chiang, Hsiao Chi; Wang, Hsuan Hui; Hseu, You Cheng

doi:10.1016/j.fct.2006.04.006

Cited by 108 publications

(78 citation statements)

References 57 publications

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“…The modifications of the membrane proteins were determined by SDS-PAGE as described previously [60,61]. The membrane proteins were isolated by Membrane and Cytosol Protein Extraction Kit (Beyotime, Nantong, China).…”

Section: Analysis Of Membrane Proteinsmentioning

confidence: 99%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

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a b s t r a c tThe present study explored the apoptosis pathways in hydroxyl radicals ( • OH)-induced carp erythrocytes. Carp erythrocytes were treated with the caspase inhibitors in physiological carp saline (PCS) or Ca 2 þ -free PCS in the presence of 40 μM FeSO 4 /20 μM H 2 O 2 . The results showed that the generation of reactive oxygen species (ROS), the release of cytochrome c and DNA fragmentation were caspase-dependent, and Ca 2 þ was involved in calpain activation and phosphatidylserine (PS) exposure in • OH-induced carp erythrocytes. Moreover, the results suggested that caspases were involved in PS exposure, and Ca 2 þ was involved in DNA fragmentation in • OH-induced fish erythrocytes. These results demonstrated that there might be two apoptosis pathways in fish erythrocytes, one is the caspase and cytochrome c-dependent apoptosis that is similar to that in mammal nucleated cells, the other is the Ca 2 þ -involved apoptosis that was similar to that in mammal non-nucleated erythrocytes. So, fish erythrocytes may be used as a model for studying oxidative stress and apoptosis in mammal cells. Furthermore, the present study investigated the effects of glutamine (Gln)'s metabolites [alanine (Ala), citrulline (Cit), proline (Pro) and their combination (Ala 10 Pro 4 Cit 1 )] on the pathways of apoptosis in fish erythrocytes. The results displayed that Ala, Cit, Pro and Ala 10 Pro 4 Cit 1 effectively suppressed ROS generation, cytochrome c release, activation of caspase-3, caspase-8 and caspase-9 at the physiological concentrations, prevented Ca 2 þ influx, calpain activation, PS exposure, DNA fragmentation and the degradation of the cytoskeleton and oxidation of membrane and hemoglobin (Hb) and increased activity of anti-hydroxyl radical (AHR) in • OH-induced carp erythrocytes. Ala 10 Pro 4 Cit 1 produced a synergistic effect of inhibited oxidative stress and apoptosis in fish erythrocytes. These results demonstrated that Ala, Cit, Pro and their combination can protect mammal erythrocytes and nucleated cells against oxidative stress and apoptosis. The studies supported the use of Gln, Ala, Cit and Pro as oxidative stress and apoptosis inhibitors in mammal cells and the hypothesis that the inhibited effects of Gln on oxidative stress and apoptosis are at least partly dependent on that of its metabolites in mammalian.

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Section: Analysis Of Membrane Proteinsmentioning

confidence: 99%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

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“…The animals were sacrificed under anesthesia and blood was collected by heart puncture in heparinized tubes. Erythrocytes were isolated and stored according to the method described by Yuan et al (Yuan et al 2005) and Yang et al (Yang et al 2006). Briefly blood samples collected were centrifuged (1500×g, 10 min) at 4ºC, erythrocytes were separated from the plasma and buffy coat and were washed three times by centrifugation (1500×g, 5 min) in 10 volumes of 10 mM phosphate buffered saline (pH 7.4; PBS).…”

Section: Preparation Of Rat Erythrocytesmentioning

confidence: 99%

Antioxidant activity of flower, stem and leaf extracts of <i>Ferula gummosa</i> Boiss

Ebrahimzadeh¹,

Nabavi²,

Nabavi³

et al. 2010

Grasas y Aceites

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The Antioxidant and antihemolytic activities of hydroalcoholic extracts of the flowers, stems and leaves of the Ferula gummosa Boiss were investigated employing different in vitro assay systems. Leaf extract showed better activity in DPPH radical scavenging. In addition it showed better activity in nitric oxide and H₂O₂ scavenging and Fe²⁺ chelating activity than the other parts. The extracts exhibited good antioxidant activity in linoleic acid system test but were not comparable with vitamin C (p< 0.001). The extracts showed weak reducing power activity. The F. gummosa stem extract showed better antihemolytic activity against H²O² induced hemolysis. Among the extracts, the flowers had higher phenolic and flavonoid contents. This plant is very promising for further biochemical experiments.

La actividad antihemolítica y antioxidante de extractos hidroalcohólicos de flores, tallos y hojas de Ferula gummosa Boiss fueron investigados empleando diferentes ensayos in vitro. Los extractos de hojas mostraron una mejor actividad captadora de radicales libres. Además, mostraron una mejor actividad captadora de óxido nítrico y H₂O₂ y actividad quelatante de Fe²⁺ que las otras partes. Los extractos exhibieron una buena actividad antioxidante en el ensayo con ácido linoleico pero no comparable con la vitamina C (p< 0.001). Los extractos mostraron una débil actividad de poder reductor. Los extractos de tallos de F. gummosa mostraron una mejor actividad antihemolítica contra la hemolisis inducida con H₂O₂. Entre los extractos, las flores tienen los más altos contenidos de fenoles y flavonoides. Esta planta es muy prometedora para futuros experimentos bioquímicos

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“…The importance to verify the potential of V. cauliflora extracts to minimize the lipid peroxidation is primordially because this event is one of the main consequences of oxidative damage, it has been involved in cell aging, suggested as a general mechanism for cell injury and death, in the last instance, it has been associated with a variety of pathological events (Hseu et al, 2002;Yang et al, 2006). In our study, flower extract was more efficient in inhibiting lipid peroxidation than stem bark extract and presented an IC 50 value almost three times lower (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Stem bark and flower extracts ofVismia caulifloraare highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes

Ribeiro

Berto

Ribeiro

et al. 2015

Pharmaceutical Biology

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(2015) Stem bark and flower extracts of Vismiacauliflora are highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes, Pharmaceutical Biology, 53:11, 1691-1698, DOI: 10.3109/13880209.2014 Previous research on V. cauliflora extracts suggests its potential to neutralize cellular oxidative damages related to the production of reactive oxygen and nitrogen species.Objective: To determine the activity of stem bark and flower extracts of V. cauliflora on the modulation of oxidative burst in human neutrophils, as well as its potential to inhibit oxidative damage in human erythrocytes. Materials and methods:The modulation of neutrophil's oxidative burst by the ethanolic extracts (0.3-1000 mg/mL) was determined by the oxidation of specific probes by reactive species. Additionally, the potential of these extracts to inhibit oxidative damage in human erythrocytes was evaluated by monitoring its biomarkers of oxidative stress. Results: Vismia cauliflora extracts presented remarkable capacity to prevent the oxidative burst in activated human neutrophils (IC 50 515 mg/mL). However, the maximum percentage of inhibition achieved against hydrogen peroxide was 45%. Concerning the oxidative damage in human erythrocytes, the extracts were able to minimize the tert-butyl hydroperoxide-induced hemoglobin oxidation and lipid peroxidation in a very low concentration range (2.7-18 mg/mL). Furthermore, only stem bark extract (100 mg/mL) was able to inhibit the depletion of glutathione (13%). Discussion and conclusion: These results reinforce the therapeutic potential of stem bark and flower extracts of V. cauliflora to heal topical skin disease, namely in the treatment of neutrophil-related dermatosis and skin conditions related to oxidative stress, including skin aging.

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Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes

Cited by 108 publications

References 57 publications

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Antioxidant activity of flower, stem and leaf extracts of <i>Ferula gummosa</i> Boiss

Stem bark and flower extracts ofVismia caulifloraare highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes

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