Protection promoted by pGP3 or pGP4 against Chlamydia muridarum is mediated by CD4+ cells in C57BL/6N mice

Mosolygó, Tímea; Szabó, Ágnes; Balogh, Emese; Faludi, Ildiko; Virók, Dezső; Endrész, Valéria; Samu, Antal; Krenács, Tibor; Burián, Katalin

doi:10.1016/j.vaccine.2014.07.039

Cited by 14 publications

(12 citation statements)

References 27 publications

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“…These data suggest that the specific-CPSIT_p7 antibodies exert no protective effect against C. psittaci infection. Similar results were observed in previous experiments [16,26]. These results demonstrated that CPSIT_p7-immunized mice elicited a powerful Th1 immune response, which suggests that the specific-CPSIT_p7 Fig.…”

Section: Discussionsupporting

confidence: 91%

“…Immunization with the pORF5 DNA vaccine induced protective immunity against genital C. trachomatis infection in a murine model [27]. Recombinant Pgp3 protein-immunized mice exhibited a significantly lower C. muridarum burden [16]. These results suggest that the role of Pgp3 of C. psittaci is similar to C. trachomatis and C. muridarum.…”

Section: Discussionmentioning

confidence: 80%

“…Notably, human antibodies recognize Pgp3 as an important immunodominant antigen in C. trachomatis infection [15]. Immunization with Pgp3 protein induces protective immunity against C. trachomatis infection in a mouse model [16]. However, the role of CPSIT_p7 (Pgp3) in C. psittaci is not clear.…”

Section: Introductionmentioning

confidence: 99%

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Immunization with Chlamydia psittaci plasmid-encoded protein CPSIT_p7 induces partial protective immunity against chlamydia lung infection in mice

Tan

Yang

et al. 2018

Immunol Res

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The present study evaluated the immune-protective efficacy of the Chlamydia psittaci (C. psittaci) plasmid protein CPSIT_p7 and analyzed the potential mechanisms of this protection. The current study used recombinant CPSIT_p7 protein with Freund's complete adjuvant and Freund's incomplete adjuvant to vaccinate BALB/c mice. Adjuvants alone or PBS formulated with the same adjuvants was used as negative controls. Mice were intranasally challenged with 10 inclusion-forming units (IFU) of C. psittaci. We found that CPSIT_p7 vaccination significantly decreased the mouse lung chlamydial load, interferon-γ (IFN-γ) level, and pathological injury. This protection correlated well with specific humoral and cellular immune responses against C. psittaci. In vitro or in vivo neutralization of C. psittaci with sera harvested from immunized mice did not reduce the number of recoverable C. psittaci in the infected lungs, but CD4 spleen cells collected from CPSIT_p7-immunized mice significantly decreased the chlamydial load via adoptive transfer to native mice. These results reveal that the protection conferred by CPSIT_p7 is dependent on CD4 T cells.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 80%

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Immunization with Chlamydia psittaci plasmid-encoded protein CPSIT_p7 induces partial protective immunity against chlamydia lung infection in mice

Tan

Yang

et al. 2018

Immunol Res

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“…Due to the immunodominance in individuals infected with C. trachomatis [65, 66], pGP3 has been evaluated as a biomarker for monitoring the C. trachomatis exposure in humans [67, 68]. Furthermore, immunization of mice with pgp3 gene or pGP3 protein has been shown to induce protective immunity [69–71]. However, assessing the role of the plasmid-encoded pGP3 in chlamydial pathogenesis was made possible only after the first report on the successful transformation of C. trachomatis L2 [72], which has permitted the detailed characterization of the plasmid-encoded ORFs in cell culture [17, 73, 74] and animal models [21] and encouraged the development of various genetic engineering tools for investigating chlamydial biology and pathogenesis [51, 75–78].…”

Section: Pgp3 Is a Key Virulence Factor Encoded By The Plasmidmentioning

confidence: 99%

Chlamydial Plasmid-Dependent Pathogenicity

Zhong

2017

Trends in Microbiology

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Most Chlamydia species carry a 7.5kb plasmid encoding 8 open reading frames conventionally called plasmid glycoproteins 1–8 or pGP1–8. Although the plasmid is not critical for chlamydial growth in vitro, its role in chlamydial pathogenesis is clearly demonstrated in the genital tracts of mice infected with Chlamydia muridarum, a model for investigating the human pathogen Chlamydia trachomatis. Plasmid-free C. trachomatis is also attenuated in both the mouse genital tract and nonhuman primate ocular tissue. Deficiency in pGP3 alone, which is regulated by pGP4, largely reproduced the in vivo but not in vitro phenotypes of the plasmid-free organisms, suggesting that pGP3 is a key in vivo virulence factor. The positive and negative regulations of some chromosomal genes by pGP4 and pGP5 respectively may allow the plasmid to promote chlamydial adaptation to varied animal tissue environments. The focus of this review is to summarize the progress on the pathogenic functions of the plasmid-encoded open reading frames, which may motivate further investigation of the molecular mechanisms of chlamydial pathogenicity and development of medical utility of the chlamydial plasmid system.

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“…One half of the homogenized lung sample derived from individual mice was centrifuged (10 min, 400× g) (Sorvall ® RT6000B, DuPont, Wilmington, DE, USA), after which, the serial dilutions of the supernatants were inoculated into McCoy cell monolayers and centrifuged (1 h, 800× g). After 48 h of culturing, the cells were fixed and visualized, and the inclusions were enumerated as described previously [21].…”

Section: Culturing Of C Pneumoniae From Murine Lungsmentioning

confidence: 99%

Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs

et al. 2021

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Ambroxol (Ax) is used as a mucolytics in the treatment of respiratory tract infections. Ax, at a general dose for humans, does not alter Chlamydia pneumoniae growth in mice. Therefore, we aimed to investigate the potential anti-chlamydial effect of Ax at a concentration four timed higher than that used in human medicine. Mice were infected with C. pneumoniae and 5-mg/kg Ax was administered orally. The number of recoverable C. pneumoniae inclusion-forming units (IFUs) in Ax-treated mice was significantly lower than that in untreated mice. mRNA expression levels of several cytokines, including interleukin 12 (IL-12), IL-23, IL-17F, interferon gamma (IFN-γ), and surfactant protein (SP)-A, increased in infected mice treated with Ax. The IFN-γ protein expression levels were also significantly higher in infected and Ax-treated mice. Furthermore, the in vitro results suggested that the ERK 1/2 activity was decreased, which is essential for the C. pneumoniae replication. SP-A and SP-D treatments significantly decreased the number of viable C. pneumoniae IFUs and significantly increased the attachment of C. pneumoniae to macrophage cells. Based on our results, a dose of 5 mg/kg of Ax exhibited an anti-chlamydial effect in mice, probably an immunomodulating effect, and may be used as supporting drug in respiratory infections caused by C. pneumoniae.

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Protection promoted by pGP3 or pGP4 against Chlamydia muridarum is mediated by CD4+ cells in C57BL/6N mice

Cited by 14 publications

References 27 publications

Immunization with Chlamydia psittaci plasmid-encoded protein CPSIT_p7 induces partial protective immunity against chlamydia lung infection in mice

Immunization with Chlamydia psittaci plasmid-encoded protein CPSIT_p7 induces partial protective immunity against chlamydia lung infection in mice

Chlamydial Plasmid-Dependent Pathogenicity

Ambroxol Treatment Suppresses the Proliferation of Chlamydia pneumoniae in Murine Lungs

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